Agrobacterium-mediated transformation of Javanica rice

Dong, Jiaqi; Teng, Weimin; Buchholz, Wallace G.; Hall, Timothy C.

doi:10.1007/bf00564204

Cited by 124 publications

(60 citation statements)

References 29 publications

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“…In the present study, genotypic differences in callus induction, subculture, and plant regeneration of Javanica rice cultivars were examined using C-modified medium (Rachmawati et al 2004) and MS2 medium (Dong et al 1996) As results, significant difference was found in callus induction rate and quality of callus induced among the five genotypes of Javanica rice (Table 2). Although a considerable variation was observed, all genotypes of rice can induce callus on both media.…”

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confidence: 83%

Studies on callus induction, plant regeneration and transformation of Javanica rice cultivars

Rachmawati

Anzai

2006

Plant Biotechnology

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The purpose of this study was to develop a reproducible efficient procedure for the transformation of Javanica rice cultivars from Indonesia. Five rice cultivars cultivated in Indonesia now were examined for their capacity on the callus growth, plant regeneration and transformation. Their potential was affected by genotype and medium. Regarding the quality of callus, type I calli produced higher plant regeneration frequency than type II calli. So type I calli were inoculated with A. tumefaciens harboring binary plasmid pAFT14, which had a hygromycin resistance (hpt) gene and a gus gene. In this study, we examined two media for two steps in the tissue culture process for transformation, i.e. callus inducing and plant regeneration. The results show that C-modified medium was the suitable media for callus induction in the most Javanica rice cultivars. Agrobacterium-mediated transformation system has been extended to five Javanica rice cultivars. Among them, Rojolele consistently gave the best performance.Key words: Callus, Javanica rice, transformation.Plant Biotechnology 23, 521-524 (2006) This article can be found at http://www.jspcmb.jp/ Tissue Culture Note tolerant to drought (gogo rice). The callus induction rate was calculated after 4 weeks of culture as the percentage of the seeds producing callus.As results, significant difference was found in callus induction rate and quality of callus induced among the five genotypes of Javanica rice (Table 2). Although a considerable variation was observed, all genotypes of rice can induce callus on both media. Two types of calli, embryogenic and non-embryogenic ones, were obtained from the embryo scutellum. The embryogenic calli (type-I) were relatively dry, milky white in color, compact and nodular in appearance ( Figure 1A, B). In contrast, non-embryogenic calli (type-II) were soft and watery, mostly white but some brown in color ( Figure 1C, D). Four weeks after induction, the calli were subcultured onto fresh medium, which had the same composition as that used for callus induction. Following the subculture, the type-I calli grew about 5 to 10-fold in volume 30 days after transfer. However, the type-II calli turned brown and died.In general, callus induction rate of the five Javanica rice cultivars on C-modified medium was higher than that on MS2 medium (Table 2). This finding is consistent with the results reported by Ogawa (2000) that Cmedium is applicable to a wider range of cultivars including Indica, Japonica and Javanica subspecies. The induction rate of type-I calli from all the five Javanica rice cultivars on C-modified medium was higher than that on MS2 medium, especially for Rojolele. On Cmodified medium, the induction rate of Rojolele was 10 times higher than one on MS2. On the other hand, the induction rate of type-II calli of the five cultivars on MS2 medium was almost the same as that on C-modified medium (Table 2). To determine the regeneration ability, the initiated calli were transferred to a regeneration medium which was composed of ma...

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confidence: 83%

Studies on callus induction, plant regeneration and transformation of Javanica rice cultivars

Rachmawati

Anzai

2006

Plant Biotechnology

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“…Agrobacterium-mediated gene transfer has been reported in all three subspecies of rice (Aldemita and Hodges, 1996;Dong et al, 1996;Hiei et al, 1994). Different insect-resistance genes, which confer resistance to major pests, have been identified from sources such as bacteria and heterologous plant species for transferring them into cultivated crops (Estruch et al, 1997).…”

Section: Among the Available Genetic Transformation Methodsmentioning

confidence: 99%

Transgenic indica rice resistant to sap‐sucking insects

Nagadhara

Ramesh

Pasalu

et al. 2003

Plant Biotechnology Journal

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SummaryAgrobacterium-mediated genetic transformation has been optimized in indica rice susceptible to sap-sucking insects, viz., brown planthopper (BPH) and green leafhopper (GLH). Snowdrop lectin gene ( gna) from Galanthus nivalis, driven by phloem-specific rice-sucrose-synthase promoter, along with herbicide resistance gene (bar) driven by CaMV 35S promoter, was employed for genetic transformation. Embryogenic calli -after cocultivation with Agrobacterium strain LBA4404 harbouring Ti plasmid pSB111-bar-gnawere selected on the medium containing phosphinothricin. PCR and Southern blot analyses confirmed the stable integration of both the genes into genomes of transgenic (T 0 ) rice plants. Northern and Western blot analyses revealed the expression of gna in the transgenic plants. In the T 1 and T 2 generations, the gna and bar transgenes showed co-segregation at a ratio of 3 : 1. Plant progenies expressing gna, in T 1 and T 2 , exhibited substantial resistance against BPH and GLH pests. This is the first report dealing with transgenic indica rice exhibiting high resistance to both insects.

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“…Arabidopsis ( Arabidopsis thaliana ) plants also transformed (Bechtold & Pelletier, 1998) with 35S-mGFP5er were kindly provided by Tao Wang (Texas A&M University, TX, USA) and rice ( Oryza sativa ) plants transformed (Dong et al ., 1996) with 35S-mGFP5er were generously donated by Yiming Jiang ( Texas A&M University, TX, USA).…”

Section: Plant Materialsmentioning

confidence: 99%

The dark side of green fluorescent protein

et al. 2005

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Summary• Here, severe interference of chlorophyll with green fluorescent protein (GFP) fluorescence is described for medicago ( Medicago truncatula ), rice ( Oryza sativa ) and arabidopsis ( Arabidopsis thaliana ). This interference disrupts the proportional relationship between GFP content and fluorescence that is intrinsic to its use as a quantitative reporter.• The involvement of chlorophyll in the loss of GFP fluorescence with leaf age was shown in vivo , by the removal of chlorophyll through etiolation or by ethanol extraction, and in vitro , by titration of a GFP solution with chlorophyll solutions of various concentrations.• A substantial decrease in fluorescence in early development of medicago and rice leaves correlated with chlorophyll accumulation. In all three species tested, removal of chlorophyll yielded up to a 10-fold increase in fluorescence. Loss of GFP fluorescence in vitro was 4-fold greater for chlorophyll b than for chlorophyll a.• Differences exist between plant species for the discrepancy between apparent GFP fluorescence and its actual level in green tissues. Substantial errors in estimating promoter activity from GFP fluorescence can occur if pigment interference is not considered.

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Agrobacterium-mediated transformation of Javanica rice

Cited by 124 publications

References 29 publications

Studies on callus induction, plant regeneration and transformation of Javanica rice cultivars

Studies on callus induction, plant regeneration and transformation of Javanica rice cultivars

Transgenic indica rice resistant to sap‐sucking insects

The dark side of green fluorescent protein

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