Agrobacterium-mediated transformation of Ceratocystis albifundus

Draft genomes of Penicillium roqueforti, Fusarium sororula, Chalaropsis populi, and Chrysoporthe puriensis are presented. Penicillium roqueforti is a model fungus for genetics, physiological and metabolic studies, as well as for biotechnological applications. Fusarium sororula and Chrysoporthe puriensis are important tree pathogens, and Chalaropsis populi is a soil-borne root-pathogen. The genome sequences presented here thus contribute towards a better understanding of both the pathogenicity and biotechnological potential of these species.

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“…They will also provide a basis for further functional studies (e.g. Sayari et al 2019;Wilson et al 2020) in Ceratocystidaceae.…”

Section: Resultsmentioning

confidence: 99%

IMA genome - F14

et al. 2021

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“…The Gene D8.t287 knockout experiment consisted of a resistance marker screen, the construction of a knockout vector, and the transformation and verification of the mutant strain. The neo gene and hyg gene respectively conferring genetin (G418) and hygromycin B (Hyg) resistance were used as the selector markers for transformed fungus [28]. Spores of the strain D were prepared by inoculation of the fungus onto PDA plates, followed by cultivation on PDA supplemented with G418 (at final concentrations of 0, 25, 50, 100, 150 and 200 µg•mL −1 ) and Hyg (at final concentrations of 0, 25, 50, 100, 150 and 200 µg•mL −1 ).…”

Section: Screening Resistance Markersmentioning

confidence: 99%

Aromatic Polyketides from a Symbiotic Strain Aspergillus fumigatus D and Characterization of Their Biosynthetic Gene D8.t287

Hua

Pan

et al. 2020

Marine Drugs

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The chemical investigation of one symbiotic strain, Aspergillus fumigatus D, from the coastal plant Edgeworthia chrysantha Lindl led to the isolation of eight compounds (1–8), which were respectively identified as rubrofusarin B (1), alternariol 9-O-methyl ether (2), fonsecinone D (3), asperpyrone A (4), asperpyrone D (5), fonsecinone B (6), fonsecinone A (7), and aurasperone A (8) by a combination of spectroscopic methods (1D NMR and ESI-MS) as well as by comparison with the literature data. An antimicrobial assay showed that these aromatic polyketides exhibited no remarkable inhibitory effect on Escherichia coli, Staphyloccocus aureus and Candida albicans. The genomic feature of strain D was analyzed, as well as its biosynthetic gene clusters, using antibiotics and Secondary Metabolite Analysis Shell 5.1.2 (antiSMASH). Plausible biosynthetic pathways for dimeric naphtho-γ-pyrones 3–8 were first proposed in this work. A non-reducing polyketide synthase (PKS) gene D8.t287 responsible for the biosynthesis of these aromatic polyketides 1–8 was identified and characterized by target gene knockout experiment and UPLC-MS analysis.

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“…Recently, an Agrobacterium ‐mediated transformation protocol was established for Ceratocystis albifundus , a filamentous ascomycete in the family Ceratocystidaceae (Sayari, van der Nest, Steenkamp, Adegeye, et al, 2019). This species is an important pathogen on plantations of Acacia mearnsii and cultivated Protea cynaroides plants in South Africa (de Beer et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…The aim of this study was to transform eight Ceratocystidaceae species using the Agrobacterium ‐mediated transformation system established in C . albifundus (Sayari, van der Nest, Steenkamp, Adegeye, et al, 2019). The existing protocol was first minorly adjusted and applied to C .…”

Section: Introductionmentioning

confidence: 99%

Transferring an Agrobacterium‐mediated transformation protocol across eight genera in the Ceratocystidaceae

et al. 2021

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Establishing a transformation system is an essential first step for functional studies. Numerous transformation systems have been developed and optimized for filamentous ascomycetes, including Agrobacterium‐mediated transformation. Recently, such a system was developed for the African tree pathogen Ceratocystis albifundus. This fungus is part of the family Ceratocystidaceae, which includes many tree and plant pathogens of economic importance. Despite the many advantages associated with Agrobacterium‐mediated transformation, establishing and optimizing this system within a species are extremely arduous due to the many species‐specific parameters that must be optimized. This study aimed to apply the existing C. albifundus Agrobacterium‐mediated transformation protocol to eight diverse Ceratocystidaceae species from eight different genera. Minor adjustments were made to the already established protocol to increase transformant yield before it was used to successfully produce transformants for six of the eight targeted species without the need for any further species‐specific optimizations. Although the number of transformants obtained was less than for C. albifundus, this study proves that protocols for Agrobacterium‐mediated transformation might be more transferable than previously thought. It also provides a useful benchmark for future transformation studies in the Ceratocystidaceae and will make species‐specific optimization easier.

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Agrobacterium-mediated transformation of Ceratocystis albifundus

Cited by 20 publications

References 57 publications

IMA genome - F14

IMA genome - F14

Aromatic Polyketides from a Symbiotic Strain Aspergillus fumigatus D and Characterization of Their Biosynthetic Gene D8.t287

Transferring an Agrobacterium‐mediated transformation protocol across eight genera in the Ceratocystidaceae

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