Agrobacterium-mediated transformation and assessment of factors influencing transgene expression in loblolly pine (Pinus taeda L.)

Tang, Wei

doi:10.1038/sj.cr.7290092

Cited by 14 publications

(11 citation statements)

References 20 publications

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“…It was reported that the initial culture on the medium without antibiotic promoted active proliferation of cultures of Pinus strobus L. (Levée et al 1999), Picea abies (Klimaszewska et al 2001) and Pinus taeda (Tang 2001). One week after culture on the selection medium, the transformed cells started to regenerate.…”

Section: Resultsmentioning

confidence: 96%

Agrobacterium-mediated transformation of embryogenic tissues of hybrid firs (Abies spp.) and regeneration of transgenic emblings

et al. 2009

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A genetic transformation system has been developed for selected embryogenic cell lines of hybrids Abies alba x A. cephalonica (cell lines AC2, AC78) and Abies alba x A. numidica (cell line AN72) using Agrobacterium tumefaciens. The cell lines were derived from immature or mature zygotic embryos on DCR medium containing BA (1 mg l(-1)). The T-DNA of plant transformation vector contained the beta-glucuronidase reporter gene under the control of double dCaMV 35S promoter and the neomycin phosphotransferase selection marker gene driven by the nos promoter. The regeneration of putative transformed tissues started approximately 1 week after transfer to the selection medium containing 10 mg geneticin l(-1). GUS activity was detected in most of the geneticin-resistant sub-lines AN72, AC2 and AC78, and the transgenic nature of embryogenic cell lines was confirmed by PCR approach. Plantlet regeneration from PCR-positive embryogenic tissues has been obtained as well. The presence of both gus and nptII genes was confirmed in 11 out of 36 analysed emblings.

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Section: Resultsmentioning

confidence: 96%

Agrobacterium-mediated transformation of embryogenic tissues of hybrid firs (Abies spp.) and regeneration of transgenic emblings

et al. 2009

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“…Ninety transgenic plants were regenerated from hygromycinresistant calli derived from families WO3, 8-1082 and 11-1029, and 19 transgenic plantlets were established in soil. The presence of the uidA gene in the plant genome was confirmed by polymerase chain reaction, Southern blot, and plant DNA/T-DNA junction analysis (Tang 2001;Tang et al 2001).…”

Section: Regeneration Of Transgenic Plantsmentioning

confidence: 99%

Genetic transformation of conifers and its application in forest biotechnology

Tang

Newton

2003

Plant Cell Reports

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Genetic modification of conifers through gene transfer technology is now an important field in forest biotechnology. Two basic methodologies, particle bombardment and Agrobacterium-mediated transformation, have been used on conifers. The use of particle bombardment has produced stable transgenic plants in Picea abies, P. glauca, P. mariana, and Pinus radiata. Transgenic plants have been produced from Larix decidua, Picea abies, P. glauca, P. mariana, Pinus strobus, P. taeda, and P. radiata via Agrobacterium-mediated transformation. Agrobacterium-mediated transformation has advantages over particle bombardment such as a simpler integration pattern and a limited rearrangement in the introduced DNA. At present, genetic transformation of conifers has been directed toward improving growth rate, wood properties and quality, pest resistance, stress tolerance, and herbicide resistance, which will drive forestry to enter a new era of productivity and quality.

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“…It would have been easier to determine false positive results, if the vector sequence contained marker genes from more than one organism. However, pBI121 is a popular binary vector and has recently been used by researchers for several plants including peanut (Rohini and Rao 2001;Sharma and Anjaiah 2000), shallot (Zheng et al 2001), spruce (Le et al 2001), buckwheat (Kojima et al 2000), poppy (Park and Facchini 2000) and pine (Tang 2001). pBI121 has also been used for in planta transformation of cactus (Seol et al 2008).…”

Section: Discussionmentioning

confidence: 98%

Tissue culture independent transformation for Corchorus olitorius

Sajib

Islam

Reza

et al. 2008

Plant Cell Tiss Organ Cult

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In vitro regeneration is difficult for species of Corchorus and several transformation attempts based on tissue culture have failed. We describe a successful transformation protocol for C. olitorius using a technique independent of tissue culture. Primary growth of a plant is brought about from the activities of meristematic region and subsequent division and differentiation of the derivative cells into the tissues and organs of the plant. The principal behind the current study was that if some dividing cells in the meristematic region become transformed by Agrobacterium and the cells retain the capacity for cell division, then the transgene(s) will be transferred to progeny cells, and if some of these cells later differentiate to form floral buds, seeds generated from these buds will inherit the transgene(s) to next generation. In this study young jute plants were transformed at shoot apical meristematic region using Agrobacterium tumefaciens. Heritable transmission of the transgene to progeny from genetically modified plants was confirmed by gus gene expression by histochemical analysis, selection on kanamycin containing medium, RT-PCR, PCR amplification and Southern hybridization. Efficiency of transformation was determined by selection on medium containing kanamycin, and inheritance of transgene to T 2 generation plants.

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Agrobacterium-mediated transformation and assessment of factors influencing transgene expression in loblolly pine (Pinus taeda L.)

Cited by 14 publications

References 20 publications

Agrobacterium-mediated transformation of embryogenic tissues of hybrid firs (Abies spp.) and regeneration of transgenic emblings

Agrobacterium-mediated transformation of embryogenic tissues of hybrid firs (Abies spp.) and regeneration of transgenic emblings

Genetic transformation of conifers and its application in forest biotechnology

Tissue culture independent transformation for Corchorus olitorius

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