Agrobacterium-mediated genetic transformation of selected tropical inbred and hybrid maize (Zea mays L.) lines

Abstract

The study was carried out to evaluate the amenability of tropical inbred and hybrid maize lines, using Agrobacterium mediated transformation technique. Agrobacterium tumefaciens strains EHA101 harbouring apTF102 binary vector, EHA101, AGL1, and LBA4404 harbouring pBECK2000.4 plasmid, LBA4404, GV and EHA105 harbouring pCAMBIA2301 plasmid, and

<b>Increased transient genetic transformation <i>in immature</i> embryos of Brazilian BR 451 maize co-cultivated with <i>Agrobacterium tumefaciens

“…The blue spots represent the location where the reporter gene was inserted in the embryo and the number of blue spots per embryo represents the intensity of the transient genetic transformation. The most commonly used co-culture conditions for Hi-II maize genetic transformation protocols are threeday co-cultivations at 20°C (T1) (Frame et al, 2002;2011;Omer, Matheka, Ali, & Machuka, 2013;Ombori et al, 2014;Nahampun et al, 2016). In our analysis, a longer period of co-cultivation (five days) or higher temperature did not increase the T-DNA transfer from bacteria to Hi-II embryos.…”

“…The first transgenic maize plants obtained by agrobacteria were reported by Ishida et al (1996) followed by other groups (Zhao et al, 2001;Frame et al, 2002;Ombori, Muoma, & Machuka, 2014;Lee & Zhang, 2016;Souza et al, 2017). To succeed in maize transformation, it is necessary to use target tissues with high rates of cell division, such as immature zygotic embryos.…”

“…While several maize genotypes have been effectively transformed (Ji, Xu, & Wang, 2013, Ombori et al, 2014Souza et al, 2017), Hi-II hybrid maize has been widely used for genetic transformation (Zhao et al, 2001;Frame et al, 2002;Frame, Main, Schick, & Wang, 2011;Lee & Zhang, 2016;Nahampun, Lopez-Arredondo, Xu, HerreraEstrella, & Wang, 2016) and is considered the model for maize plant transformation. However, the poor agronomic traits of the Hi-II hybrid (Ishida, Hiei, & Komari, 2007;Que et al, 2014) motivate the search for other maize genotypes that respond well to transformation.…”

Genetic transformation of the Brazilian BR 451 maize variety by the Agrobacterium tumefaciens method

“…Great efforts have been made to adapt gene transfer into cereals via A. tumefaciens as they are resistant to such maneuvers (FRAME et al, 2002;2011). This recalcitrance is related to two different aspects: a) the low in vitro response (CARVALHO et al, 1997;FRAME et al, 2006;GONZÁLES et al, 2012;GRANDO et al, 2013) e b) low explant sensibility to agrobacteria infection (VENNA et al, 2003;CARVALHO et al, 2004;WANG et al, 2007), thus restricting the number of transformed maize genotypes (GONZÁLEZ et al, 2012;OMBORI et al, 2014).…”

Review Article: ISTR, a Retrotransposons-Based Marker to Assess Plant Genome Variability with Special Emphasis in the Genera &lt;i&gt;Zea&lt;/i&gt; and &lt;i&gt;Agave&lt;/i&gt;

“…Besides, there are thousands of reports of genetic changes after modern techniques of biotechnology and breeding in many crops. At this time, transformation and transposon-induced changes are new ways of mutation [5][6][7][8]; transformation and somatic hybridization are new ways of recombination [9]. Techniques like embryo rescue and in vitro pollination have permitted hybridizations till now impossible [10,11].…”