Agrobacterium-mediated genetic transformation of plants: The role of host

Abstract

Agrobacterium-mediated genetic transformation is the most widely used technology to obtain overexpression of recombinant proteins in plants. Molecular events that occur within Agrobacterium during interactions with host plants have been studied extensively, and now we have a reasonable understanding the key factors involved in the regulation of T-DNA nuclear import and genomic integration. By contrast, very little is known about the events that take place in the host cells during genetic transformation by Agrobacterium. Here, we describe the plant-related factors including genotype, genes, proteins, competency of target tissues and phenolic compounds that participate in Agrobacteriummediated genetic transformation and discuss their possible roles in this process. Because Agrobacterium probably adapts existing cellular processes for its life cycle, identifying the processes in host cells during Agrobacterium infection might contribute to better understanding of basic biological processes as cell communication, intracellular transport and DNA repair and recombination as well as to expanding the host range of Agrobacterium as a genetic engineering tool.

Stable Transformation of Ferns Using Spores as Targets: Pteris vittata and Ceratopteris thalictroides

“…The choice of targets for transformation plays a crucial role in successful stable transformation by A. tumefaciens (de la Riva et al, 1998;Karami et al, 2009) and by biolistics (Kikkert et al, 2004;Jones and Sparks, 2009). When spores were transformed using bombardment, evenly GUS-stained gametophytes were observed, demonstrating that targeting spores for transformation avoids the potential for chimeras.…”

The effect of yeast extract and methyl jasmonate on rosmarinic acid accumulation in Coleus blumei hairy roots

“…plantlets cultivated in vitro were used. Hairy root cultures were established after transformation of leaf explants with Agrobacterium rhizogenes A4 (pHRI) according to Bauer et al (2002) and Karami et al (2009). Briefly, leaf segments were incubated for 15 min in 2-d-old bacteria suspension.…”

Organogenesis and plant selection by kanamycin of strawberry in vitro aiming at genetic transformation

“…Marker genes that induce resistance to substances such as antibiotics assist in transformed plant selection by inhibiting untransformed shoot growth (ABDAL-AZIZ et al, 2006;KARAMI et al, 2009;QIN et al, 2011). Many transformation methods use the nptII gene, which confers resistance to the aminoglycoside group of antibiotics (neomycin, kanamycin, and gentamicin), to transform cells (MARCELLINO et al, 2007;QIN et al, 2011;PANTAZIS et al, 2013).…”

“…Many transformation methods use the nptII gene, which confers resistance to the aminoglycoside group of antibiotics (neomycin, kanamycin, and gentamicin), to transform cells (MARCELLINO et al, 2007;QIN et al, 2011;PANTAZIS et al, 2013). However, the selective agents can have a negative effect on callus induction and regeneration capacity and vary according to the concentration, species, cultivar and explant type (KARAMI et al, 2009).…”