Agrobacterium-mediated genetic transformation of pineapple var. Queen using a novel encapsulation-based antibiotic selection technique

Gangopadhyay, Gaurab; Roy, Subhash Kanti; Gangopadhyay, Sangita Basu; Mukherjee, K. K.

doi:10.1007/s11240-009-9528-8

Cited by 14 publications

(8 citation statements)

References 10 publications

(11 reference statements)

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“…It is used to indicate transient and stable transformations (Serres et al 1992), and to estimate the relative contribution and strength of promoter elements in the transcriptional activation. Most recently, GUS histochemical assay was utilized in the production of markerfree Petunia hybrida and tomato (Khan et al , 2011, and in the production of a more efficient Agrobacterium tumefaciens-mediated transformation protocol in melon (Ntui et al 2010), in Lilium (Azadi et al 2010), in Petunia hybrida (Thirukkumaran et al 2009), in pineapple (Gangopadhyay et al 2009), in Piper colubrinum (Mani and Manjula 2011), in sesame (Yadav et al 2010), in…”

Section: Discussionmentioning

confidence: 99%

Characterization of inhibitor(s) of β-glucuronidase enzyme activity in GUS-transgenic wheat

Ramadan

Eissa

El-Domyati

et al. 2011

Plant Cell Tiss Organ Cult

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The uidA gene, encoding for b-glucuronidase (GUS), is the most frequently used reporter gene in plants.As a reporter enzyme, GUS can be assayed both qualitatively and quantitatively. In wheat, there are numerous reports of failure in detecting GUS enzyme activity in tissues of transgenic plants, while other reports have suggested presence of b-glucuronidase inhibitor(s) in wheat tissues. In the present study, we show that the b-glucuronidase enzyme activity is not only tissue-specific but also genotype-dependent. Our data demonstrate that the glucuronic acid could be the candidate inhibitor for b-glucuronidase enzyme activity in wheat leaves and roots. It should be noted that the assays to detect b-glucuronidase enzyme activity in wheat should be interpreted carefully. Based on the data of our present study, we recommend studying the chemical pathways, the unintended effects and the possible loss-of-function of any candidate transgene prior to transformation experiments.

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Section: Discussionmentioning

confidence: 99%

Characterization of inhibitor(s) of β-glucuronidase enzyme activity in GUS-transgenic wheat

Ramadan

Eissa

El-Domyati

et al. 2011

Plant Cell Tiss Organ Cult

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“…In the past, it has been found that, under appropriate in vitro conditions, the intrinsic and induced capability of plant cells and tissues to undergo different developmental stages of somatic embryogenesis is extremely critical (Zhang et al 2007). Several studies on in vitro regeneration by somatic embryogenesis in pineapple have been reported (Daquinta et al 1997;Firoozabady and Moy 2004;Rahman et al 2005;Firoozabady et al 2006;Gangopadhyay et al 2009). MS medium supplemented with decreasing concentration gradient of glutamine, KNO 3 and NH 4 NO 3 contributed to induce somatic embryogenesis in recalcitrant cotton cultivars (Ikram-ul-haq and Yusuf 2004).…”

Section: Introductionmentioning

confidence: 99%

Regeneration of pineapple (Ananas comosus L.) plant through somatic embryogenesis

Yapo

Kouakou

Koné

et al. 2011

J. Plant Biochem. Biotechnol.

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An efficient protocol for a complete plant regeneration by somatic embryogenesis was developed with Smooth Cayenne pineapple (Ananas comosus L.).Previous works showed that this species is responsive to somatic embryogenesis. In the present work the influence of components of culture medium in the induction, development and conversion of somatic embryos was investigate in order to establish a somatic embryogenesis protocol. Nodular callus (83.67%) was initiated from leaf explants of young plants on CIM 3 medium. The highest frequency (37.6%) of embryogenic callus induction was obtained from 4-week-old calluses on EIM 3 medium supplemented with 3.0 mg/l picloram. The highly organized callus induction and the development of somatic embryos were achieved after the transfer of callus clumps onto EIM 3 medium containing 1.0 mg/l BAP + 0.1 mg/l NAA. The frequency of somatic embryo formation was of 39.5± 2.45 embryos per callus. Up to 97% of the somatic embryos were converted into complete plants within 4 week on MSB medium with 1.0 mg/l BAP + 0.05 mg/l GA 3 + 500 mg/l glutamine. The continuation of the elongation of the shoots occurred on this medium). Shoots obtained from all the above methods were rooted in MSB medium with activated charcoal. Complete plantlets were transferred onto specially made polyethylene bags containing soil mixture and transferred to the greenhouse. Survival rate of the plantlets under ex vitro conditions was 98% and maximum average number of plantlets (80±0.6). The well-developed plantlets were transferred to an open field where the plants produced normal fruits.

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“…Preliminary results are available as conference abstracts relating to the transformation of pineapple using both Agrobacterium and microprojectile-mediated gene transfer [3,4,8,9,11,14]. Recently, Gangopadhyay et al [10] reported Agrobacterium-mediated genetic transformation of pineapple cv. "Queen" using a novel encapsulation-based antibiotic selection technique.…”

Section: Estimation Of Iron and Zinc In Transformed Linesmentioning

confidence: 99%

Enhanced Iron and Zinc Accumulation in Genetically Engineered Pineapple Plants Using Soybean Ferritin Gene

Mhatre

Srinivas

Ganapathi

2011

Biol Trace Elem Res

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Pineapple (Ananas comosus L. Merr., cv. "Queen") leaf bases were transformed with Agrobacterium tumefaciens strain EHA 105 harboring the pSF and pEFESF plasmids with soybean ferritin cDNA. Four to eight percent of the co-cultivated leaf bases produced multiple shoots 6 weeks after transfer to Murashige and Skoog's medium supplemented with α-naphthalene acetic acid 1.8 mg/l, indole-3-butyric acid 2.0 mg/l, kinetin 2.0 mg/l, cefotaxime 400 mg/l, and kanamycin 50 mg/l. Putatively transformed shoots (1-2 cm) were selected and multiplied on medium of the same composition and elongated shoots (5 cm) were rooted on liquid rooting medium supplemented with cefotaxime 400 mg/l and kanamycin 100 mg/l. The rooted plants were analyzed through PCR, genomic Southern analysis, and reverse transcription PCR. The results clearly confirmed the integration and expression of soybean ferritin gene in the transformed plants. Atomic absorption spectroscopic analysis carried out with six independently transformed lines of pSF and pEFE-SF revealed a maximum of 5.03-fold increase in iron and 2.44-fold increase in zinc accumulation in the leaves of pSF-transformed plants. In pEFE-SF-transformed plants, a 3.65-fold increase in iron and 2.05-fold increase in zinc levels was observed. Few of the transgenic plants were hardened in the greenhouse and are being grown to maturity to determine the enhanced iron and zinc accumulation in the fruits. To the best of our knowledge this is the first report on the transformation of pineapple with soybean ferritin for enhanced accumulation of iron and zinc content in the transgenic plants.

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Agrobacterium-mediated genetic transformation of pineapple var. Queen using a novel encapsulation-based antibiotic selection technique

Cited by 14 publications

References 10 publications

Characterization of inhibitor(s) of β-glucuronidase enzyme activity in GUS-transgenic wheat

Characterization of inhibitor(s) of β-glucuronidase enzyme activity in GUS-transgenic wheat

Regeneration of pineapple (Ananas comosus L.) plant through somatic embryogenesis

Enhanced Iron and Zinc Accumulation in Genetically Engineered Pineapple Plants Using Soybean Ferritin Gene

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