Agrobacterium-Mediated Genetic Transformation Competence of Different Prunus Explants, as Revealed by Gus Expression

Pratesi, D.; Paris, Roberta; Negri, P.; Scotti, Adalgisa

doi:10.17660/actahortic.2004.663.87

Cited by 4 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the genetic transformation of Prunus is difficult because it is recalcitrant to in vitro regeneration. Although transformation systems using Agrobacterium have been developed in several fruit tree species of Prunus, including apricot (P. armeniaca) (Laimer et al, 1992;Petri et al, 2004;Pratesi et al, 2004), almond (P. dulcis) (Ainsley et al, 2001;Archilletti et al, 1995;Miguel and Oliveira, 1999), sweet cherry (P. avium) (Druart et al, 1998;Pratesi et al, 2004), sour cherry (P. cerasus) (Dolgov and Firsov, 1999;Song and Sink, 2006), European plum (P. domestica) (Mante et al, 1991;Scorza et al, 1994;Scorza et al, 1995a, b;Tian et al, 2009;Yancheva et al, 2002), peach (P. persica) (Hammerschlag and Smigocki, 1998;Pérez-Clemente et al, 2004;Ye et al, 1994), Chinese plum (P. salicina) (Urtubia et al, 2008), and Japanese apricot (P. mume) (Gao et al, 2010), most of them were transformed with reporter genes and few were successfully transformed with agriculturally useful transgenes. Furthermore, the genetic transformation efficiency reported was usually low in Prunus.…”

Section: Introductionmentioning

confidence: 99%

Improving Infection Efficiency of Agrobacterium to Immature Cotyledon Explants of Japanese Apricot (Prunus mume) by Sonication Treatment

Gao–Takai

Tao

2014

J. Japan. Soc. Hort. Sci.

View full text Add to dashboard Cite

The infection efficiency of Agrobacterium to cotyledon explants of Japanese apricot (Prunus mume) was markedly improved by sonication treatment. The use of sGFP(S65T) reporter gene in this study enabled direct observation of transgene expression, thus allowing the evaluation of Agrobacterium infection efficiency. Immature cotyledons of different cultivars and different developmental stages were subjected to sonication treatment of durations ranging from 10 sec to 2 min. When early-stage immature cotyledons of 'Nanko' were subjected to sonication treatment, the transient GFP expression frequency after co-cultivation was nearly 100% and GFP fluorescence was distributed over almost the entire cotyledon surface. In contrast, when the inoculation was carried out according to the standard dipping method, transient GFP expression frequency was less than 10% and GFP fluorescence was observed only in spots. The highest frequency of somatic embryogenesis (SEG) with GFP expression was obtained from 40 sec sonication treatment of May 14 'Nanko' immature cotyledons. However, with younger cotyledons, which have a higher SEG frequency, 20 sec of sonication treatment was found to be sufficient to increase the infection frequency.

show abstract

Section: Introductionmentioning

confidence: 99%

Improving Infection Efficiency of Agrobacterium to Immature Cotyledon Explants of Japanese Apricot (Prunus mume) by Sonication Treatment

Gao–Takai

Tao

2014

J. Japan. Soc. Hort. Sci.

View full text Add to dashboard Cite

show abstract

Cherries

Song

Lang

Sink

et al. 2008

Compendium of Transgenic Crop Plants

View full text Add to dashboard Cite

Genetic transformation will be an increasingly important tool for potential improvement of cultivated cherry species (e.g., Prunus avium L., P. cerasus L., and interspecific rootstocks). To understand the best potential applications of genetic engineering and transformation to overcome current genetic barriers to, and/or improve efficiency of, developing improved genotypes for fruit production and rootstock development, the review begins with pertinent background on cherry cultivation, including species history, origins, botanical features, ploidy levels and genetic linkages, and economic and nutritional importance. Traditional breeding objectives, tools, strategies, and achievements to date are discussed, as well as the limitations of conventional breeding, for sweet cherry, sour cherry, and cherry rootstocks. Subsequently, current information on state‐of‐the‐art cherry regeneration and transformation systems is presented. Most cherry species have been recalcitrant to transformation, primarily due to in vitro regeneration difficulties. Consequently, the various regeneration systems that have been attempted are discussed, including descriptions of the biotic and abiotic factors that have been found to influence plant regeneration, such as genotype and explants, culture media, plant growth regulators, and environmental conditions. Current cherry transformation systems are described, including gene delivery systems, explant tissues for transformation, selection regimes, and regeneration of transgenic plants. Based on the perspective of priorities for transgenes, the review concludes with an outline of some targeted traits for future cherry improvement via genetic transformation, including resistance to specific diseases and insects as well as horticultural characteristics.

show abstract

Technological advances in temperate hardwood tree improvement including breeding and molecular marker applications

Pijut

Woeste

Vengadesan

et al. 2007

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

Hardwood forests and plantations are an important economic resource for the forest products industry worldwide and to the international trade of lumber and logs. Hardwood trees are also planted for ecological reasons, for example, wildlife habitat, native woodland restoration, and riparian buffers. The demand for quality hardwood from tree plantations will continue to rise as the worldwide consumption of forest products increases. Tree improvement of temperate hardwoods has lagged behind that of coniferous species and hardwoods of the genera Populus and Eucalyptus. The development of marker systems has become an almost necessary complement to the classical breeding and improvement of hardwood tree populations for superior growth, form, and timber characteristics. Molecular markers are especially valuable for determining the reproductive biology and population structure of natural forests and plantations, and the identity of genes affecting quantitative traits. Clonal reproduction of commercially important hardwood tree species provides improved planting stock for use in progeny testing and production forestry. Development of in vitro and conventional vegetative propagation methods allows mass production of clones of mature, elite genotypes or genetically improved genotypes.Genetic modification of hardwood tree species could potentially produce trees with herbicide tolerance, disease and pest resistance, improved wood quality, and reproductive manipulations for commercial plantations. This review concentrates on recent advances in conventional breeding and selection, molecular marker application, in vitro culture, and genetic transformation, and discusses the future challenges and opportunities for valuable temperate (or "fine") hardwood tree improvement.

show abstract

Agrobacterium-Mediated Genetic Transformation Competence of Different Prunus Explants, as Revealed by Gus Expression

Cited by 4 publications

References 0 publications

Improving Infection Efficiency of Agrobacterium to Immature Cotyledon Explants of Japanese Apricot (Prunus mume) by Sonication Treatment

Improving Infection Efficiency of Agrobacterium to Immature Cotyledon Explants of Japanese Apricot (Prunus mume) by Sonication Treatment

Cherries

Technological advances in temperate hardwood tree improvement including breeding and molecular marker applications

Contact Info

Product

Resources

About