Agrobacterium-mediated delivery of CRISPR/Cas reagents for genome editing in plants enters an era of ternary vector systems

Zhang, Yu; Zhang, Qiang; Chen, Qijun

doi:10.1007/s11427-020-1685-9

Cited by 17 publications

(8 citation statements)

References 43 publications

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“…Double peaks represent heterozygous or chimeric mutations, and an asterisk indicates a mutation induced by PE. The first mutation in #15 is the unwanted mutation introduced by the pegRNA scaffold vectors [10,11], pZ1PE3 and pZ1PE3b, for the same P165S mutation that are based on two different strategies, PE3 and PE3b [1], respectively (Fig. 1a, b).…”

Section: Resultsmentioning

confidence: 99%

Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize

et al. 2020

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Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines. We hypothesize that enhancing pegRNA expression could improve prime-editing efficiency. In this report, we describe two strategies for enhancing pegRNA expression. We construct a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2. Compared with previous reports in rice, we achieve much higher prime-editing efficiency in maize. Our results are inspiring and provide a direction for the optimization of plant prime editors.

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Section: Resultsmentioning

confidence: 99%

Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize

et al. 2020

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“…We used the maize Ubi1 promoter to drive the expression of maize codon-optimized PE2 and the OsU3 and TaU3 promoters to drive the expression of pegRNA and sgRNA, respectively. In this way, we constructed two pGreen3 binary vectors [10,11], pZ1PE3 and pZ1PE3b, for the same P165S mutation that are based on two different strategies, PE3 and PE3b [1], respectively (Fig. 1a, b).…”

Section: Resultsmentioning

confidence: 99%

Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize

Jiang

Chai

et al. 2020

Preprint

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AbstractA novel and universal CRISPR/Cas-derived precision genome editing technology named prime editing was developed. However, low prime editing efficiency was shown in transgenic rice lines. We reasoned that enhancing pegRNA expression would be able to improve prime editing efficiency. In this report, we used two strategies to enhance pegRNA expression and constructed a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2. As compared with previous reports in rice, we achieved much higher prime editing efficiency in maize. Our results are inspiring and provide a direction for optimization of plant prime editors.

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“…The establishment of the system depends on transformation and plant tissue culture. Genetic transformation can be performed in many ways, such as Agrobacterium ‐mediated methods, microprojectile bombardment, microinjection, the pollen–tube pathway, and ion irradiation (Zhang et al., 2020d). The Agrobacterium ‐mediated method is most frequently used owing to the low cost and ease of performance.…”

Section: Genetic Engineering Is An Effective Methods For Breeding Superior Varietiesmentioning

confidence: 99%

Combining novel technologies with interdisciplinary basic research to enhance horticultural crops

et al. 2021

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SUMMARY Horticultural crops mainly include fruits, vegetables, ornamental trees and flowers, and tea trees (Melaleuca alternifolia). They produce a variety of nutrients for the daily human diet in addition to the nutrition provided by staple crops, and some of them additionally possess ornamental and medicinal features. As such, horticultural crops make unique and important contributions to both food security and a colorful lifestyle. Under the current climate change scenario, the growing population and limited arable land means that agriculture, and especially horticulture, has been facing unprecedented challenges to meet the diverse demands of human daily life. Breeding horticultural crops with high quality and adaptability and establishing an effective system that combines cultivation, post‐harvest handling, and sales becomes increasingly imperative for horticultural production. This review discusses characteristic and recent research highlights in horticultural crops, focusing on the breeding of quality traits and the mechanisms that underpin them. It additionally addresses challenges and potential solutions in horticultural production and post‐harvest practices. Finally, we provide a prospective as to how emerging technologies can be implemented alongside interdisciplinary basic research to enhance our understanding and exploitation of horticultural crops.

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Agrobacterium-mediated delivery of CRISPR/Cas reagents for genome editing in plants enters an era of ternary vector systems

Cited by 17 publications

References 43 publications

Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize

Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize

Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize

Combining novel technologies with interdisciplinary basic research to enhance horticultural crops

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