Agrobacterium-mediated co-transformation of a pea β-1,3-glucanase and chitinase genes in potato (Solanum tuberosum L. c.v. Russet Burbank) using a single selectable marker

Chang, Ming‐Mei; Culley, David E.; Choi, Jane J.; Hadwiger, Lee A.

doi:10.1016/s0168-9452(02)00063-8

Cited by 43 publications

(10 citation statements)

References 19 publications

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“…In potatoes (Solanum tuberosum L.), different approaches have been tried to obtain efficient in vitro regeneration system from petioles with intact leaflets (Yee et al, 2001), leaves (Sarker and Mustafa, 2002;Andersson et al, 2003), leaf discs (Osusky et al, 2005), tuber discs (Vasquez and Clarence, 2002), and from stem (Chang et al, 2002) through callus induction. However, somaclonal variation in plants coupled with long regeneration period have been reported from potato plants derived from leaf discs (Fleming et al, 1992;Trujillo et al, 2001), stem segments (Cardi et al, 1993), anthers (Véronneau et al, 1992) and protoplasts (SreeRamulu et al, 1983;Coleman et al, 1990) and these have been reported to be genotype dependent.…”

Section: Introductionmentioning

confidence: 99%

In vitro somatic embryogenesis and regeneration potential of two potato varieties in Uganda

Byarugaba¹,

Magembe²,

Barekye³

et al. 2018

Afr. J. Biotechnol.

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Section: Introductionmentioning

confidence: 99%

In vitro somatic embryogenesis and regeneration potential of two potato varieties in Uganda

Byarugaba¹,

Magembe²,

Barekye³

et al. 2018

Afr. J. Biotechnol.

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“…In potato, as far as we know, only two publications have reported the introduction and expression of the ChiC gene. One report has described the introduction and expression of pea b-1,3-glucanase and ChiC genes using a single selectable marker, the neomycin phosphotransferase (nptII) gene (Chang et al 2002). However, the resistance assay of the transgenic plants has not been described.…”

Section: Introductionmentioning

confidence: 99%

Production of transgenic potato exhibiting enhanced resistance to fungal infections and herbicide applications

Khan

Sjahril

Nakamura

et al. 2008

Plant Biotechnol Rep

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Potato (Solanum tuberosum L.), one of the most important food crops, is susceptible to a number of devastating fungal pathogens in addition to bacterial and other pathogens. Producing disease-resistant cultivars has been an effective and useful strategy to combat the attack of pathogens. Potato was transformed with Agrobacterium tumefaciens strain EHA101 harboring chitinase, (ChiC) isolated from Streptomyces griseus strain HUT 6037 and bialaphos resistance (bar) genes in a binary plasmid vector, pEKH1. Polymerase chain reaction (PCR) analysis revealed that the ChiC and bar genes are integrated into the genome of transgenic plants. Different insertion sites of the transgenes (one to six sites for ChiC and three to seven for bar) were indicated by Southern blot analysis of genomic DNA from the transgenic plants. Expression of the ChiC gene at the messenger RNA (mRNA) level was confirmed by Northern blot analysis and that of the bar gene by herbicide resistance assay. The results obviously confirmed that the ChiC and bar genes are successfully integrated and expressed into the genome, resulting in the production of bialaphos-resistant transgenic plants. Disease-resistance assay of the in vitro and greenhouse-grown transgenic plants demonstrated enhanced resistance against the fungal pathogen Alternaria solani (causal agent of early blight).

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“…Plant regeneration and transformation in potato has been reported using a wide range of explants including leaves, stems, tubers, and microtubers (Mitten et al, 1990;Visser, 1991;Snyder and Belknap, 1993;Dale and Hampton, 1995;Chang et al, 2002); using a variety of germplasm, including diploids, tetraploids, and Solanum species (Visser et al, 1989;Kumar et al, 1995;Park et al, 1995;Beaujean et al, 1998;Trujillo et al, 2001); and using several different methods (De Block, 1988;Wenzler et al, 1989;Sidorov et al, 1999;Romano et al, 2001). The number of plants produced is usually low for most varieties and dependent on the genotype used (Wenzler et al, 1989;Yee et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Transformation and Plant Regeneration from Leaf Explants of Solanum tuberosum L. cv. ‘Shepody’

Gustafson

Mallubhotla

MacDonnell³

et al. 2006

Plant Cell Tiss Organ Cult

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As part of a large-scale genomics project focused on understanding and improving the Shepody potato, we have increased the regeneration and transformation rates for this cultivar. Using combinations of auxins and trans-zeatin, leaf and stem explants were evaluated for callus induction and shoot formation. Several plant growth regulator combinations resulted in higher plant regeneration rates over a previous method. Using the best combination of auxin and cytokinin in combination with Agrobacterium-mediated transformation, we regenerated independent putative transformants from 59.5% of the total explants plated. We ran PCR on a sample of the plants to confirm transformation and 47.1% were nptII positive; giving a confirmed transformation rate of 28.0%.

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Agrobacterium-mediated co-transformation of a pea β-1,3-glucanase and chitinase genes in potato (Solanum tuberosum L. c.v. Russet Burbank) using a single selectable marker

Cited by 43 publications

References 19 publications

In vitro somatic embryogenesis and regeneration potential of two potato varieties in Uganda

In vitro somatic embryogenesis and regeneration potential of two potato varieties in Uganda

Production of transgenic potato exhibiting enhanced resistance to fungal infections and herbicide applications

Transformation and Plant Regeneration from Leaf Explants of Solanum tuberosum L. cv. ‘Shepody’

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