Agro-industrial wastes for the synthesis of carotenoids by Xanthophyllomyces dendrorhous: Mesquite pods-based medium design and optimization

Villegas-Méndez, Miguel Ángel; Aguilar-Machado, Diederich; Balagurusamy, Nagamani; Montañez, Julio; Morales‐Oyervides, Lourdes

doi:10.1016/j.bej.2019.107260

Cited by 29 publications

(11 citation statements)

References 41 publications

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“…Moisture, pH, and carbon-to-nitrogen ratio were evaluated in the carotenoids production by R. glutinis using rice bran (Roadjanakamolson and Suntornsuk, 2010). The Taguchi methodology was applied to optimize the production of carotenoids by Xanthophyllomyces dendrorhous, using four variables: concentrations of mesquite pods extract, corn steep liquor, yeast extract, and malt extract (Villegas-Méndez et al, 2019).…”

Section: Modes Of Fermentation and Scale Of Productionmentioning

confidence: 99%

Agro-Industrial Residues: Eco-Friendly and Inexpensive Substrates for Microbial Pigments Production

Lopes

Ligabue-Braun

2021

Front. Sustain. Food Syst.

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Many commodities are abundantly produced around the world, including soybean, corn, rice sugarcane, cassava, coffee, fruits, and many others. These productions are responsible for the generation of enormous amounts of daily residues, such as cassava and sugarcane bagasses, rice husk, and coffee peel. These residues are rich sources for renewable energy and can be used as substrates for industrial interest products. Microorganisms are useful biofactories, capable of producing important primary and secondary metabolites, including alcohol, enzymes, antibiotics, pigments, and many other molecules. The production of pigments was reported in bacteria, filamentous fungi, yeasts, and algae. These natural microbial pigments are very promising because synthetic colorants present a long history of allergies and toxicity. In addition, many natural pigments present other biological activities, such as antioxidant and antimicrobial activities, that are interesting for industrial applications. The use of inexpensive substrates for the production of these metabolites is very attractive, considering that agro-industrial residues are generated in high amounts and usually are a problem to the industry. Therefore, in this article we review the production of microbial pigments using agro-industrial residues during the current decade (2010–2020), considering both submerged and solid state fermentations, wild-type and genetically modified microorganisms, laboratorial to large-scale bioprocesses, and other possible biological activities related to these pigments.

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Section: Modes Of Fermentation and Scale Of Productionmentioning

confidence: 99%

Agro-Industrial Residues: Eco-Friendly and Inexpensive Substrates for Microbial Pigments Production

Lopes

Ligabue-Braun

2021

Front. Sustain. Food Syst.

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“…Since candelilla by-products could be a rich source of bioactive compounds with possible applications in the industry, scientific projects in recent years have focused on the revaluation of residues through the integral use of residual chemistry in the cell matrix [ 7 ] or the application of additional processes that allow the generation of organic molecules of interest through solid-state fermentation processes [ 8 , 9 ]. According to the literature, candelilla by-products contain polyphenolic compounds such as ellagitannins and ellagic acid [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant activity of polyphenolic compounds obtained from Euphorbia antisyphilitica by-products

Bautista-Hernández

Aranda-Ledesma

Rojas

et al. 2021

Heliyon

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In this study, an alternative extraction technique, ultrasound-assisted extraction, was used to extract the polyphenolic fraction from two different residues of the candelilla plant ( Euphorbia antisyphilitica ). These metabolites were further analyzed to evaluate their bioactivity as antioxidants. In addition, their functional groups were identified by Fourier transform infrared (FTIR) spectroscopy. The antioxidant assays showed statistically significant differences between the phenolic extracts, with citric acid residues (CAR) exhibiting a higher oxidant effect than sulfuric acid residues (SAR). The CAR from San Jerónimo (SJ) cultivar showed decreased IC 50 values (179.441 ± 7.92 μL mL −1 , DPPH • ), and its polyphenolic fraction was able to inhibit lipid oxidation (70.31 ± 2.50%). FTIR analysis subsequently revealed the presence of functional groups related to polyphenolic compounds, such as hydroxy, carbonyl, carbon double bond, and amine groups. In addition, FTIR spectra showed slight differences in phenolic compounds, due to the strong acid treatment involved in the extraction of wax. The present study demonstrated that candelilla by-products from citric acid-wax extraction have a polyphenolic fraction with strong antioxidant activity, which may be useful in food and pharmaceutical products.

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“…The yeast X. dendrorhous synthesize the all-trans isomer mainly, but the 9-cis and 13-cis isomers can be also found ( Schmidt et al, 2011 ). The high-rate cell growth with short cultivation cycles, the ability to metabolize waste-based culture media, no seasonal conditions constraints and the easy manipulation of culture conditions to improve yields make the yeast X. dendrorhous an alternative for large scale production of natural astaxanthin ( Wu et al, 2018 ; Villegas-Méndez et al, 2019 ). Generally astaxanthin production from naturally isolated strains of X. dendrorhous is very low (between 200 and 400 μg/g), however, some authors have developed genetic manipulations to get hyper-producer strains with yields up to 10-fold higher than the wild-type strains ( Visser et al, 2003 ; Zhuang et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Processes Triggered by PEF for Astaxanthin Extraction From Xanthophyllomyces dendrorhous

Aguilar-Machado

Delso

Martínez

et al. 2020

Front. Bioeng. Biotechnol.

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The aim of this study was to evaluate the potential of pulsed electric fields (PEF) to improve the extraction of the lipid-soluble astaxanthin from fresh biomass of a wild-type (CECT 11028) and mutant (ATCC 74219) Xanthophyllomyces dendrorhous strain using ethanol as solvent. Inactivation and propidium uptake studies revealed that inactivation is a good index for estimated the proportion of irreversible permeabilized cells when inactivation is higher than 70% in the two strains. Ethanol was ineffective for extracting carotenoids from the PEF-treated cells (20 kV/cm, 135 µs) of the two strains. However, after aqueous incubation of PEF-treated X. dendrorhous ATCC 74219 cells for 12 h, up to 2.4 ± 0.05 mg/g dried weight (d.w.) of carotenoids were extracted in ethanol. From total carotenoid extracted, around 84% corresponded to all-trans astaxanthin. The detection and quantification of esterase activity in the supernatant and the relationship between the percentage of esterase activity quantified and the amount of carotenoids extracted indicate that the extraction of astaxanthin was mediated by enzymatic esterase activity triggered by PEF during incubation. On the other hand, the formation of a large lipid globule into the cytoplasm of PEF-treated X. dendrorhous CECT 11028 cells during aqueous incubation prevented carotenoid extraction. The process developed in this investigation represents a more sustainable and greener method that those previously used for extracting astaxanthin from yeast.

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Agro-industrial wastes for the synthesis of carotenoids by Xanthophyllomyces dendrorhous: Mesquite pods-based medium design and optimization

Cited by 29 publications

References 41 publications

Agro-Industrial Residues: Eco-Friendly and Inexpensive Substrates for Microbial Pigments Production

Agro-Industrial Residues: Eco-Friendly and Inexpensive Substrates for Microbial Pigments Production

Antioxidant activity of polyphenolic compounds obtained from Euphorbia antisyphilitica by-products

Enzymatic Processes Triggered by PEF for Astaxanthin Extraction From Xanthophyllomyces dendrorhous

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