Agro-Industrial Wastes: A Substrate for Multi-Enzymes Production by Cryphonectria parasitica

Savino, Salvatore; Bulgari, Daniela; Monti, Eugenio; Gobbi, Emanuela

doi:10.3390/fermentation7040279

Cited by 9 publications

(4 citation statements)

References 68 publications

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“…Esterase and cellulase production in the SSF process seems to be associated with the fungal growth phase, likely due to the metabolism activation of the microorganisms in the presence of a nutrient-rich substrate as previously reported. 48,49 Although Trichoderma spp. have laccase and protease in their enzymatic machinery, the activity of these two enzymes was not observed in the tested conditions.…”

Section: ■ Discussionmentioning

confidence: 99%

Solid-State Fermentation of Trichoderma spp.: A New Way to Valorize the Agricultural Digestate and Produce Value-Added Bioproducts

Bulgari

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Peron

et al. 2023

J. Agric. Food Chem.

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In this study, the agricultural digestate from anaerobic biogas production mixed with food wastes was used as a substrate to grow Trichoderma reesei RUT-C30 and Trichoderma atroviride Ta13 in solid-state fermentation (SSF) and produce highvalue bioproducts, such as bioactive molecules to be used as ingredients for biostimulants. The Trichoderma spp. reached their maximum growth after 6 and 3 SSF days, respectively. Both Trichoderma species were able to produce cellulase, esterase, and citric and malic acids, while T. atroviride also produced gibberellins and oxylipins as shown by ultraperformance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) profiling. Experimental evaluation of germination parameters highlighted a significant promotion of tomato seed germination and root elongation induced by T. atroviride crude extracts from SSF. This study suggests an innovative sustainable use of the whole digestate mixed with agro-food waste as a valuable substrate in fungal biorefineries. Here, it has been applied to produce plant growth-promoting fungi and bioactive molecules for sustainable agriculture.

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Section: ■ Discussionmentioning

confidence: 99%

Solid-State Fermentation of Trichoderma spp.: A New Way to Valorize the Agricultural Digestate and Produce Value-Added Bioproducts

Bulgari

Alias

Peron

et al. 2023

J. Agric. Food Chem.

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“…The carboxylesterase activity (EC 3.1.1.1) was determined spectrophotometrically by measuring the hydrolysis of 4-nitrophenyl butyrate (pNPB, Merck, Sigma-Aldrich, Darmstadt, Germany) as a substrate, as previously reported [9]. Ten µL of crude extract was used as an enzyme source.…”

Section: Esterase Activitymentioning

confidence: 99%

“…Cereal wastes and residues are the most investigated substrates for enzyme production in SSF, while the most common enzymes reported are xylanases, followed by cellulase, β-glucosidase, laccase and β-xylosidase [7]. Few studies have been carried out on esterase/lipase production in SSF; usually, different waste-based substrates such as digestate and wheat bran, with supplements, have been used with fungi belonging to the genera Trichoderma, Aspergillus, Cryphonectria and Rhizophus [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Esterase Production in Solid-State Fermentation of Agricultural Digestate

et al. 2023

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The continuous increase in biogas production poses the need for innovative applications for its by-products. Solid-state fermentation (SSF) has regained attention in the development of several products because of the possibility to use low-cost and easily available substrates, such as organic wastes. SSF represents a valuable process for agricultural digestate valorization in terms of enzyme production. In the present study, cellulase and esterase were produced by Trichoderma asperellum R on a digestate-based substrate in SSF, with esterase as the highest obtained activity. After assessing the effect of light on it, the esterase production in SSF was optimized using response surface methodology. The influence of substrate composition, temperature and humidity on the enzyme production was evaluated on two sets of data generated based on digestate concentration (50% and 70% w/w). The statistical analyses revealed that these parameters affected esterase production only when Trichoderma asperellum grew on substrate containing 50% w/w of digestate. The best esterase activity (264.6 mU/mg total protein) was achieved with the following optimized SSF parameters: 50% digestate, 50% fruits, 10% sawdust, 30 °C. The current finding of esterase production on digestate-based substrates makes the SSF method presented here a sustainable and completely circular technology.

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“…producing multiple enzymes are required, as are protocols for the concurrent production of multiple enzymes[52] under SSF activity was detectable after only 24h of incubation and increased rapidly to attaint maximum activity of 85. 24 ± 1.13 IU/mL after five days of fermentation and decreased thereafter.…”

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confidence: 99%

The study of the industrial aptitude of Aspergillus fumigatus strain for xylanase production

et al. 2023

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The major microorganisms used in the industry are fungi. The production of fungal metabolites on a large scale needs the use of an industrial-scale efficient strain. Indeed, the microorganisms used in an industrial process must be capable of doing more than simply producing the desired product in high yield. In fact, one of the main bottlenecks in the fermentation procedure is the scaling-up process. Indeed, reproducing the best conditions for microorganism growth and metabolism achieved in small cultures when transferring them to a much larger fermentation scale is nearly impossible. Aspergillus fumigatus (ON226990) performance was firstly evaluated in large-scale cultures; batch fermentation using simple sugar (xylose) as substrate in various volumes; 250 mL flasks, 2 L and 20 L bioreactors. The effect of agitation speed on the morphological aspects of this strain was also investigated. Subsequent fermentations were carried out with optimal agitation, using xylan from corncob as substrate in 250 mL flasks and a 2 L bioreactor. Finally, the strain's ability to use low-cost substrate such as Alfa biomass for xylanase production was evaluated as a biotechnological application.

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Agro-Industrial Wastes: A Substrate for Multi-Enzymes Production by Cryphonectria parasitica

Cited by 9 publications

References 68 publications

Solid-State Fermentation of Trichoderma spp.: A New Way to Valorize the Agricultural Digestate and Produce Value-Added Bioproducts

Solid-State Fermentation of Trichoderma spp.: A New Way to Valorize the Agricultural Digestate and Produce Value-Added Bioproducts

Optimization of Esterase Production in Solid-State Fermentation of Agricultural Digestate

The study of the industrial aptitude of Aspergillus fumigatus strain for xylanase production

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