Agro-tourism is a tourism concept that becomes an alternative to natural tourism which has an important meaning in improving and developing the local economy and social community in a country, and the concept of Agro-tourism is also important for economic improvement, especially for developing countries. However, changes and decreases in interest in Agro-tourism sometimes occur, therefore it is important to know and understand what factors affect the sustainability of Agro-tourism. The method used in this study is the Systematic Literature Review (SLR) method to identify, review, evaluate and interpret all available research with interesting phenomenon topic areas, with certain relevant research questions. This paper explores 30 Scopus papers that have been selected according to the topic and discussion of the sustainability of ago-tourism. The findings of this paper about the methodology, analysis tools, and place of research that dominated appear from 2012-2022 are presented in this paper. In addition, this paper also presents the finding on the factors that affect sustainability in Agro-tourism.