Agricultural Soils Amended With Thermally-Dried Anaerobically-Digested Sewage Sludge Showed Increased Risk of Antibiotic Resistance Dissemination

Jauregi, Leire; Epelde, Lur; Alkorta, Itziar; Garbisu, Carlos

doi:10.3389/fmicb.2021.666854

Cited by 17 publications

(9 citation statements)

References 95 publications

(123 reference statements)

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“…In addition, manure application significantly increased the total abundance of ARGs and intl1 at all food chain levels. Manure application significantly increased the abundance of ARGs in agricultural soils, which is consistent with most previous studies (He, et al 2020;Jauregi, et al 2021). This is mainly because manure application on the one hand can directly increase the input of exogenous carbon and provide sufficient nutrients for bacteria, thus causing an increase in the number of soil bacteria, which in turn promotes the increase in the number and relative abundance of ARGs carried by resistant bacteria in the soil.…”

Section: The Distribution Of Args and Intl1 In Each Sector Of The Soi...supporting

confidence: 90%

Spread of Plasmids Carrying Antibiotic Resistance Genes in Soil-Lettuce-Snail Food Chain

Zhang,

Ji,

Tang

et al. 2023

Preprint

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Agricultural activities such as fertilization change the composition of antibiotic resistance genes(ARGs) and their host bacteria in agricultural fields, while complex microbial activities contribute to the transfer of ARGs and their host bacteria into crops and their transmission to humans through the consumption of agricultural products. The spread of plasmid-mediated ARGs in the farm food chain is unknown, as is the risk of their transfer to human pathogenic bacteria. Therefore, this study constructed a farmland food chain with soil-lettuce-snail as a typical structure, added genetically engineered Pseudomonas fluorescens containing multidrug-resistant plasmid RP4 to track its spread in the farmland food chain, and used different fertilization methods (no fertilization, chemical fertilizer application and manure application) to explore its influence on the spread and diffusion of ARGs and intl1 in the farmland food chain. It was found that exogenous Pseudomonas can enter plants from soil and pass into snails' intestines, and there is horizontal gene transfer phenomenon of RP4 plasmid in bacteria. The addition of exogenous drug-resistant bacteria had different effects on the total abundance of ARGs and intl1 at different interfaces of the food chain, but fertilization, especially manure, not only promoted the spread of Pseudomonas aeruginosa and the transfer of RP4 plasmid levels, but also significantly increased the total abundance of ARGs and intl1 at all interfaces of the food chain. The main ARGs host bacteria in the food chain include Proteobacteria, Bacteroides and Firmicutes, while Flavobacterium of Bacteroides is the unique potential host bacteria of RP4 plasmid, and fertilization obviously increases the abundance of Bacteroides. To sum up, this study provides reference for the risk assessment of ARGs spreading to human body through food chain.

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Section: The Distribution Of Args and Intl1 In Each Sector Of The Soi...supporting

confidence: 90%

Spread of Plasmids Carrying Antibiotic Resistance Genes in Soil-Lettuce-Snail Food Chain

Zhang,

Ji,

Tang

et al. 2023

Preprint

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“…Heavy metals in biosolids and other wastes exist, including after treatment in the form of a molecule or ion, thus ensuring horizontal gene transfer between different bacteria ( Ezugworie et al, 2021 ). Recently, thermally dried anaerobically digested sewage sludge has been proved as a source of ARGs and mobile genetic elements (MGEs), thus increasing the risk of antibiotic resistance dissemination in agricultural soils ( Jauregi et al, 2021 ).…”

Section: Risks Of Traditional Microorganism-bearing Fertilizersmentioning

confidence: 99%

Safety Level of Microorganism-Bearing Products Applied in Soil-Plant Systems

Mocali¹,

Canfora²,

Malusà

et al. 2022

Front. Plant Sci.

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The indiscriminate use of chemical fertilizers adversely affects ecological health and soil microbiota provoking loss of soil fertility and greater pathogen and pest presence in soil-plant systems, which further reduce the quality of food and human health. Therefore, the sustainability, circular economy, environmental safety of agricultural production, and health concerns made possible the practical realization of eco-friendly biotechnological approaches like organic matter amendments, biofertilizers, biopesticides, and reuse of agro-industrial wastes by applying novel and traditional methods and processes. However, the advancement in the field of Biotechnology/Agriculture is related to the safety of these microorganism-bearing products. While the existing regulations in this field are well-known and are applied in the preparation and application of waste organic matter and microbial inoculants, more attention should be paid to gene transfer, antibiotic resistance, contamination of the workers and environment in farms and biotech-plants, and microbiome changes. These risks should be carefully assessed, and new analytical tools and regulations should be applied to ensure safe and high-quality food and a healthy environment for people working in the field of bio-based soil amendments.

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“…Therefore, there is a widespread concern that spreading sludge on farmland would contribute to the development or spread of antibiotic resistance [ 89 , 90 ]. Antibiotics can accumulate in food webs and, even more alarmingly, antibiotic resistance genes can be transferred between environmental bacteria and human pathogens [ 91 ]. Antibiotics and their effects on the environment have become an important theme in environmental science.…”

Section: Ecological Effect An Environmental Risk Assessmentmentioning

confidence: 99%

From Sewage Sludge to the Soil—Transfer of Pharmaceuticals: A Review

Bolesta

Głodniok

Styszko

2022

IJERPH

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Sewage sludge, produced in the process of wastewater treatment and managed for agriculture, poses the risk of disseminating all the pollutants contained in it. It is tested for heavy metals or parasites, but the concentration of pharmaceuticals in the sludge is not controlled. The presence of these micropollutants in sludge is proven and there is no doubt about their negative impact on the environment. The fate of these micropollutants in the soil is a new and important issue that needs to be known to finally assess the safety of the agricultural use of sewage sludge. The article will discuss issues related to the presence of pharmaceuticals in sewage sludge and their physicochemical properties. The changes that pharmaceuticals undergo have a significant impact on living organisms. This is important for the implementation of a circular economy, which fits perfectly into the agricultural use of stabilized sewage sludge. Research should be undertaken that clearly shows that there is no risk from pharmaceuticals or vice versa: they contribute to the strict definition of maximum allowable concentrations in sludge, which will become an additional criterion in the legislation on municipal sewage sludge.

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Agricultural Soils Amended With Thermally-Dried Anaerobically-Digested Sewage Sludge Showed Increased Risk of Antibiotic Resistance Dissemination

Cited by 17 publications

References 95 publications

Spread of Plasmids Carrying Antibiotic Resistance Genes in Soil-Lettuce-Snail Food Chain

Spread of Plasmids Carrying Antibiotic Resistance Genes in Soil-Lettuce-Snail Food Chain

Safety Level of Microorganism-Bearing Products Applied in Soil-Plant Systems

From Sewage Sludge to the Soil—Transfer of Pharmaceuticals: A Review

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