The nitrogen (N) budget calculation approach is a useful means of evaluating the impact of human activity on the N cycle. Field scale N budget calculations may ignore the interactions between landscapes, and regional scale calculations rely on statistical data and indirect parameters. Watershed scale budget calculations allow for a more direct quantification of N inputs and outputs. We conducted N budget calculations for a rice paddydominated agricultural watershed in eastern China for 2007-2009, based on intensive monitoring of stream N dynamics, atmospheric deposition, ammonia (NH 3 ) volatilization and household interviews about Nrelated agricultural activities. The results showed that although total N input to the watershed was up to 280 kg N ha -1 year -1 , riverine discharge was only 4.2 kg N ha -1 year -1 , accounting for 1.5% of the total N input, and was further reduced to 2.0 kg N ha -1 year -1 after reservoir storage and/or denitrification removal. The low riverine N output was because of the characteristics of the rice paddydominated landscape, which intercepts run-off and enhances soil denitrification. The watershed actually purified the N in rainwater, as N concentrations in river discharge were much lower than those in rain water. Major N outputs included food/feed export, NH 3 volatilization from chemical fertilizer and manure, and emissions from crop residue burning. Net reactive gaseous emissions (emissions minus deposition) accounted for 5.5% of the total N input, much higher than riverine discharge. Therefore, the agricultural N cycle in such paddy-dominated watersheds impacts the environment mainly through gas exchange rather than water discharge.Keywords Eastern China Á Nitrogen budget Á Reservoir Á Riverine discharge Á Watershed Abbreviations NANI Net anthropogenic nitrogen input N Nitrogen TN Total dissolved nitrogen