Agreement and internal quality assurance of the Neubauer hemocytometer and Makler chamber for human sperm concentration determination
Ane Francyne Costa,
Fabiana Botelho de Miranda Onofre,
Alexandre Sherlley Casimiro Onofre
Abstract:Objective: The Neubauer hemocytometer, as well as the Makler chamber, are devices commonly used in andrology laboratories. The present study aimed to verify if both methods yield comparable results, and whether they can be used interchangeably to determine sperm concentration.Methods: Sperm and latex beads concentration measurements were performed with the Neubauer hemocytometer and the Makler chamber. Fixed and proportional biases were estimated, and the method agreement was determined by assessing sperm conc… Show more
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