Agreement among evaluators of bovine embryos produced in vivo or in vitro

Farin, P.W.; Britt, J.H.; Shaw, Douglas W.; Slenning, Barrett D.

doi:10.1016/0093-691x(95)00189-f

Cited by 75 publications

(63 citation statements)

References 21 publications

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Section: Introductionmentioning

confidence: 99%

“…However, such evaluation is subjective in itself, and consent is often not reached even among specialized and experienced personnel (Farin et al 1995). Furthermore, selection of embryos based on morphology has been shown to be difficult, especially for embryos of intermediate morphological qualities (Farin et al 1995, Hasler 1998. More objective selection criteria are therefore needed (Neuber et al 2003, Scott 2003, and a better understanding of embryonic metabolism could be very useful in the development of new strategies for judging the quality and the developmental potential of individual embryos (Overström 1992, Overströ m et al 1992.…”

Section: Introductionmentioning

confidence: 99%

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Respiration rates of individual bovine in vitro-produced embryos measured with a novel, non-invasive and highly sensitive microsensor system

Lopes¹,

Larsen²,

Ramsing³

et al. 2005

Reproduction

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Oxygen consumption is a useful parameter for evaluating embryo quality, since it provides a valuable indication of overall metabolic activity. Over the years, several approaches have been used to measure the respiration rates of individual embryos, but a convincing method has not yet been reported. In this study, we introduce and have validated a novel high resolution microsensor technology to determine the respiration rates of individual embryos at different developmental stages. We have employed this technology to investigate the correlation between respiration rate and embryo morphology, diameter and sex. Following morphological evaluation, individual respiration rates of day 3 (n 5 18) and day 7 (n 5 60) bovine in vitro-produced embryos were determined. Of the measured embryos, 64 were lysed for sex diagnosis by PCR. Average respiration rates of day 7 embryos (1.30 6 0.064 nl/h) were 3.4-fold higher than day 3 embryos (0.38 6 0.011 nl/h). On day 7, the average respiration rate of quality 1 blastocysts was significantly higher than the respiration rates of the lower qualities. For both day 3 and day 7 embryos, respiration rates were directly influenced by embryo diameter but did not differ between sexes. These results have demonstrated that the novel microsensor technology can be used to accurately and rapidly (8 min) measure the respiration rates of individual embryos at different developmental stages. Respiration rates were only in partial agreement with embryo morphology, suggesting a slight discrepancy between these two methods in assessing embryo quality. It is likely that a combined assessment of embryo respiration and morphology would improve embryo classification and subsequent selection.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Respiration rates of individual bovine in vitro-produced embryos measured with a novel, non-invasive and highly sensitive microsensor system

Lopes¹,

Larsen²,

Ramsing³

et al. 2005

Reproduction

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show abstract

“…Persons evaluating embryos agreed just in 68.5% of cases, carrying out the assessment of embryos in different stages of development and various degeneration levels. It was most difficult to differentiate between embryos of good and sufficient quality (Farin et al 1995). On the other hand, no significant differences in the proportion of pregnant recipients were recorded between evaluating investigators.…”

Section: Morphological Classification Of Embryosrecent Studies and Pementioning

confidence: 97%

“…However, even upon embryo evaluation by many different people the morphological assessment of embryos proved to be a useful way to evaluate their quality since a fundamental dependence was documented between the fertilization index and embryo quality class (Donaldson 1986). The subjective nature of the morphological assessment was proved by Farin et al (1995) who compared the results of six different people, evaluating 15 embryos received in vivo and produced in vitro. The assessment of embryo quality made by them differed significantly.…”

Section: Morphological Classification Of Embryosrecent Studies and Pementioning

confidence: 99%

The invasive and new non-invasive methods of mammalian oocyte and embryo quality assessment: a review

Bukowska¹,

Kempisty²,

Piotrowska³

et al. 2012

Vet. Med. - Czech

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ABSTRACT:The quality of oocytes-embryos can be determined by several techniques, including morphological, molecular, cellular and biochemical ones. The morphological methods of female gamete or embryo quality assessment often use thе following in vitro manipulation procedures such as: in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro embryo production (IVP). However, these methods are highly subjective and the morphological classification of oocytes or embryos is not always compatible with their ability to grow and develop. Additionally, molecular biology methods are objective and present parametric results, which are more or less comparable to the real oocyte-embryo "health". Although these techniques enable us to determine markers of oocyte-embryo developmental potential, when applied they lead to destruction of the analysed cells. Therefore, the need still exists to search for new methods that will be highly objective (parametric) and, which is most important, non-invasive. In this review, the morphological and molecular methods of oocyte-embryo quality assessment are presented. Moreover, we described a new system based on microfluidic technology (Lab-on-Chip) which allows the creation of a new device for mammalian oocyte as well as embryo quality evaluation: by using their spectral characterisation following embryo transfer (ET) procedures in the cattle and the pig.

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“…In terms of cryopreservation, in vitro produced embryos have decreased survival rates post-thawing after conventional freezing and postwarming after vitrification (Papadopoulos et al, 2002). Furthermore, there are morphological differences such as an overall lower cell number of both TE and ICM compared to in vivo derived embryos (Farin et al, 1995). The increasing use of in vitro produced embryos adds a challenge to the successful outcome of ET, and prediction of embryo quality prior to transfer could result in a major improvement of pregnancy rate.…”

Section: In Vitro Produced Embryosmentioning

confidence: 99%

Basic and practical aspects of pregnancy establishment in cattle

Pedersen¹,

Mazzoni²,

Stroebech³

et al. 2017

Anim Reprod

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Bovine embryos are increasingly produced using reproductive technologies, e.g. ovum pick-up (OPU), in vitro embryo production (IVP) and embryo transfer (ET). Such in vitro manipulated embryos are known to deviate in several aspects compared to in vivo derived embryos. Pregnancy establishment in cattle involves timed biological events including fine-tuned communication, initiated and carried out by both the embryo and the endometrium. This stimulates research to increase the understanding of events and interactions taking place in the uterus after embryo transfer, both from a biological and systems biology point of view. This review will focus on the biological events taking place during early embryonic development, implantation and beginning of placentation, with focus on transfer of in vitro produced embryos, including a systems biology approach for selection of superior embryo recipients.

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Agreement among evaluators of bovine embryos produced in vivo or in vitro

Cited by 75 publications

References 21 publications

Respiration rates of individual bovine in vitro-produced embryos measured with a novel, non-invasive and highly sensitive microsensor system

Respiration rates of individual bovine in vitro-produced embryos measured with a novel, non-invasive and highly sensitive microsensor system

The invasive and new non-invasive methods of mammalian oocyte and embryo quality assessment: a review

Basic and practical aspects of pregnancy establishment in cattle

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