Agonist-promoted Ubiquitination Differentially Regulates Receptor Trafficking of Endothelin Type A and Type B Receptors

Terada, Koji; Horinouchi, Takahiro; Fujioka, Yoichiro; Higashi, Taishi; Nepal, Prabha; Horiguchi, Michiko; Karki, Sarita; Hatate, Chizuru; Hoshi, Akimasa; Harada, Tomohiro; Mai, Yosuke; Ohba, Yusuke; Miwa, Soichi

doi:10.1074/jbc.m113.544171

Cited by 14 publications

(12 citation statements)

References 44 publications

(34 reference statements)

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“…3A,B), we found that Tfn was present in most Rab4A-labeled microdomains; consistent with previous reports showing the Tfn receptor can follow both the short-and the long-loop recycling routes (Mayle et al, 2012). Other GPCRs (PAR1 and Etb), which are sorted to lysosomes (Shapiro and Coughlin, 1998;Terada et al, 2014), hardly correlated with Rab4A microdomains ( Fig. 3B; Fig.…”

Section: Flna Contributes To Early Endosomal Motilitysupporting

confidence: 80%

Phosphorylation of filamin A regulates chemokine receptor CCR2 recycling

Pons

Izquierdo

Andreu-Carbó

et al. 2017

Journal of Cell Science

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Proper endosomal trafficking of ligand-activated G-protein-coupled receptors (GPCRs) is essential to spatiotemporally tune their physiological responses. For the monocyte chemoattractant receptor 2 (CCR2B; one of two isoforms encoded by CCR2), endocytic recycling is important to sustain monocyte migration, whereas filamin A (FLNa) is essential for CCL2-induced monocyte migration. Here, we analyze the role of FLNa in the trafficking of CCR2B along the endocytic pathway. In FLNa-knockdown cells, activated CCR2B accumulated in enlarged EEA-1-positive endosomes, which exhibited slow movement and fast fluorescence recovery, suggesting an imbalance between receptor entry and exit rates. Utilizing super-resolution microscopy, we observed that FLNa-GFP, CCR2B and β2-adrenergic receptor (β2AR) were present in actin-enriched endosomal microdomains. Depletion of FLNa decreased CCR2B association with these microdomains and concomitantly delayed CCR2B endosomal traffic, without apparently affecting the number of microdomains. Interestingly, CCR2B and β2AR signaling induced phosphorylation of FLNa at residue S2152, and this phosphorylation event was contributes to sustain receptor recycling. Thus, our data strongly suggest that CCR2B and β2AR signals to FLNa to stimulate its endocytosis and recycling to the plasma membrane.

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Section: Flna Contributes To Early Endosomal Motilitysupporting

confidence: 80%

Phosphorylation of filamin A regulates chemokine receptor CCR2 recycling

Pons

Izquierdo

Andreu-Carbó

et al. 2017

Journal of Cell Science

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“…Bortezomib inhibits the activity of proteasomes, which degrade poly-ubiquitinated proteins, and thus ubiquitinated proteins accumulate in the cells [25] . Ubiquitin acts as a marker for proteins undergoing degradation and also regulates trafficking of membrane proteins [26] . Among the four types of taste cells, type III taste cells are the only cells expressing protein gene product 9.5 [27] , which can act as a ubiquitin C-terminal hydrolase [28] , [29] .…”

Section: Discussionmentioning

confidence: 99%

Bortezomib alters sour taste sensitivity in mice

et al. 2017

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“…Retroviral vector pMXrmv5/HA‐Twin‐ Strep ‐ETRs was constructed by inserting a Twin‐ Strep ‐tag between fragments encoding a HA tag and ETRs in plasmid pMXrmv5/HA‐ETRs . Vector expressing HA‐Twin‐ Strep ‐ETRs was constructed by inserting a fragment encoding HA‐Twin‐ Strep ‐ETRs into plasmid pcDNA3.1/hygro(+).…”

Section: Methodsmentioning

confidence: 99%

Endothelin type B receptor interacts with the 78‐kDa glucose‐regulated protein

et al. 2019

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Endothelin (ET)‐1 is involved in the vascular system, cell proliferation and apoptosis. ET receptors consist of ET type A receptor (ETAR) and ET type B receptor (ETBR). ETAR and ETBR generally exhibit opposite responses, although many exceptions exist. In the present study, we attempted to identify ETAR‐ or ETBR‐specific binding proteins to understand the differences in ETAR‐ and ETBR‐mediated responses after ET‐1 stimulation. The 78‐kDa glucose‐regulated protein (GRP78) showed a stronger binding affinity towards ETBR than towards ETAR. Moreover, GRP78 overexpression promoted ETBR‐mediated ERK activation and GRP78 silencing suppressed ETBR‐mediated ERK activation. Furthermore, ETBR can localize GRP78 to the cell periphery. These results suggest that the interaction of ETBR with GRP78 affects ERK activation and GRP78 localization.

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Agonist-promoted Ubiquitination Differentially Regulates Receptor Trafficking of Endothelin Type A and Type B Receptors

Cited by 14 publications

References 44 publications

Phosphorylation of filamin A regulates chemokine receptor CCR2 recycling

Phosphorylation of filamin A regulates chemokine receptor CCR2 recycling

Bortezomib alters sour taste sensitivity in mice

Endothelin type B receptor interacts with the 78‐kDa glucose‐regulated protein

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