Agonist-induced localized Ca2+ spikes directly triggering exocytotic secretion in exocrine pancreas.

Maruyama, Yoshio; Inooka, Gen; Li, Yeu Xin; Miyashita, Yasushi; Kasai, Hideaki

doi:10.1002/j.1460-2075.1993.tb05970.x

Cited by 110 publications

(78 citation statements)

References 28 publications

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“…In some cases little or no [Ca 2ϩ ] i increase at all is observed in basolateral regions (5,6). This spatial pattern of [Ca 2ϩ ] i signaling has been suggested to be important for both unidirectional fluid secretion (2, 7) and exocytosis (8).…”

mentioning

confidence: 82%

Imaging of Intracellular Calcium Stores in Individual Permeabilized Pancreatic Acinar Cells

Put¹,

Elliott²

1996

Journal of Biological Chemistry

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2؉ release in a dose-dependent, "quantal" fashion. The kinetics of this release were similar to those reported for suspensions of permeabilized pancreatic acinar cells. Interestingly, the permeabilized acinar cells showed no intercellular variation in Ins(1,4,5)P 3 sensitivity. Although SLO treatment is known to result in a considerable loss of cytosolic factors, permeabilization did not result in a redistribution of zymogen granules, as judged by electron microscope analysis. These results suggest that Ins(1,4,5)P 3 -sensitive Ca 2؉ stores are unlikely to be redistributed as a result of SLO treatment. The effects of Ins(1,4,5)P 3 were therefore subsequently studied at the subcellular level. Detailed analysis demonstrated that no regional differences in Ins(1,4,5)P 3 sensitivity exist in this permeabilized cell system. Therefore, we propose that additional cytosolic factors and/or the involvement of ryanodine receptors underlie the polarized pattern of agonist-induced Ca 2؉ signaling in intact pancreatic acinar cells.

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mentioning

confidence: 82%

Imaging of Intracellular Calcium Stores in Individual Permeabilized Pancreatic Acinar Cells

Put¹,

Elliott²

1996

Journal of Biological Chemistry

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“…In pancreatic acinar cells, C m measurements have been used to demonstrate that application of a cholinergic agonist or artificial Ca 2ϩ elevation can induce changes in cell surface area (8,15,16,22). In the current study we determined whether this technique could be used to monitor membrane surface dynamics of parotid acinar cells.…”

Section: Exocytosis Induced By Camp and Camentioning

confidence: 99%

Spatiotemporal analysis of exocytosis in mouse parotid acinar cells

Chen¹,

Warner²,

Yule³

et al. 2005

American Journal of Physiology-Cell Physiology

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Exocrine cells of the digestive system are specialized to secrete protein and fluid in response to neuronal and/or hormonal input. Although morphologically similar, parotid and pancreatic acinar cells exhibit important functional divergence in Ca2+ signaling properties. To address whether there are fundamental differences in exocytotic release of digestive enzyme from exocrine cells of salivary gland versus pancreas, we applied electrophysiological and optical methods to investigate spatial and temporal characteristics of zymogen-containing secretory granule fusion at the single-acinar cell level by direct or agonist-induced Ca2+ and cAMP elevation. Temporally resolved membrane capacitance measurements revealed that two apparent phases of exocytosis were induced by Ca2+ elevation: a rapidly activated initial phase that could not be resolved as individual fusion events and a second phase that was activated after a delay, increased in a staircaselike fashion, was augmented by cAMP elevation, and likely reflected both sequential compound and multivesicular fusion of zymogen-containing granules. Optical measurements of exocytosis with time-differential imaging analysis revealed that zymogen granule fusion was induced after a minimum delay of ∼200 ms, occurred initially at apical and basolateral borders of acinar cells, and under strong stimulation proceeded from apical pole to deeper regions of the cell interior. Zymogen granule fusions appeared to coordinate subsequent fusions and produced persistent structures that generally lasted several minutes. In addition, parotid gland slices were used to assess secretory dynamics in a more physiological context. Parotid acinar cells were shown to exhibit both similar and divergent properties compared with the better-studied pancreatic acinar cell regarding spatial organization and kinetics of exocytotic fusion of zymogen granules.

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“…A wide range of epithelial functions such as fluid and electrolyte secretion (Kasai and Augustine, 1990;Ito et al, 1997), exocytosis (Maruyama et al, 1993;Ito et al, 1997) or TJ permeability (Nathanson et al, 1992) are regulated by cytosolic Ca 2+ . Videoimaging of Ca 2+ indicators have demonstrated that, in a large number of epithelial cells, Ca 2+ signals are remarkably organized in space and often polarized.…”

Section: Introductionmentioning

confidence: 99%

Vimentin and the K-Ras-induced actin-binding protein control inositol-(1,4,5)-trisphosphate receptor redistribution during MDCK cell differentiation.

Dingli¹,

Parys²,

Loew³

et al. 2012

Journal of Cell Science

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Agonist-induced localized Ca2+ spikes directly triggering exocytotic secretion in exocrine pancreas.

Cited by 110 publications

References 28 publications

Imaging of Intracellular Calcium Stores in Individual Permeabilized Pancreatic Acinar Cells

Imaging of Intracellular Calcium Stores in Individual Permeabilized Pancreatic Acinar Cells

Spatiotemporal analysis of exocytosis in mouse parotid acinar cells

Vimentin and the K-Ras-induced actin-binding protein control inositol-(1,4,5)-trisphosphate receptor redistribution during MDCK cell differentiation.

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