AGO1 controls arabidopsis inflorescence architecture possibly by regulating TFL1 expression

Fernández-Nohales, Pedro; Domenech, M. J.; Alba, Ángel Emilio Martínez de; Micol, José Luis; Ponce, Marı́a Rosa; Madueño, Francisco

doi:10.1093/aob/mcu132

Cited by 20 publications

(18 citation statements)

References 55 publications

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“…In the F3 and F4 progeny of the latter plants, a 3:1 (Hyg r :Hyg s ) segregation was observed, confirming that they were homozygous for ago1-52 but hemizygous for amiR-MAS2.1. The phenotype of the ago1-52/ago1-52; amiR-MAS2.1/amiR-MAS2.1 lethal seedlings resembled that of plants homozygous for null alleles of AGO1, whereas the ago1-52/ago1-52; amiR-MAS2.1 plants were very similar to those homozygous for the ago1-103 mutation, a very strong but viable allele of AGO1 (Fernández-Nohales et al, 2014).…”

Section: Amir-mas21 Increases the Severity Of The Mutant Phenotype Omentioning

confidence: 93%

Arabidopsis MAS2, an Essential Gene That Encodes a Homolog of Animal NF-κ B Activating Protein, Is Involved in 45S Ribosomal DNA Silencing

et al. 2015

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Ribosome biogenesis requires stoichiometric amounts of ribosomal proteins and rRNAs. Synthesis of rRNAs consumes most of the transcriptional activity of eukaryotic cells, but its regulation remains largely unclear in plants. We conducted a screen for ethyl methanesulfonate-induced suppressors of Arabidopsis thaliana ago1-52, a hypomorphic allele of AGO1 (ARGONAUTE1), a key gene in microRNA pathways. We identified nine extragenic suppressors as alleles of MAS2 (MORPHOLOGY OF AGO1-52 SUPPRESSED2). Positional cloning showed that MAS2 encodes the putative ortholog of NKAP (NF-k B activating protein), a conserved eukaryotic protein involved in transcriptional repression and splicing in animals. The mas2 point mutations behave as informational suppressors of ago1 alleles that cause missplicing. MAS2 is a single-copy gene whose insertional alleles are embryonic lethal. In yeast two-hybrid assays, MAS2 interacted with splicing and ribosome biogenesis proteins, and fluorescence in situ hybridization showed that MAS2 colocalizes with the 45S rDNA at the nucleolar organizer regions (NORs). The artificial microRNA amiR-MAS2 partially repressed MAS2 and caused hypomethylation of 45S rDNA promoters as well as partial NOR decondensation, indicating that MAS2 negatively regulates 45S rDNA expression. Our results thus reveal a key player in the regulation of rRNA synthesis in plants.

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Section: Amir-mas21 Increases the Severity Of The Mutant Phenotype Omentioning

confidence: 93%

Arabidopsis MAS2, an Essential Gene That Encodes a Homolog of Animal NF-κ B Activating Protein, Is Involved in 45S Ribosomal DNA Silencing

et al. 2015

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“…Further analysis revealed that 37 MADS-box transcription factors may perform important functions in cotton flower development, including 5 AP1, 6 AGL6, 3 SEP2, 2 SEP3, 2 SEP4, 3 AG, 4 AP3 and 4 PI (Additional file 1 : Table S3 and Fig. 6 c), which had been demonstrated to play pivotal roles in Arabidopsis floral induction and development [ 24 , 25 ]. KEGG pathway analysis showed that these differentially expressed genes were mainly involved in secondary metabolism, plant hormone signal transduction and starch and sucrose metabolism (Additional file 1 : Figure S8).…”

Section: Resultsmentioning

confidence: 99%

Natural variation in a CENTRORADIALIS homolog contributed to cluster fruiting and early maturity in cotton

et al. 2018

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BackgroundPlant architecture and the vegetative-reproductive transition have major impacts on the agronomic success of crop plants, but genetic mechanisms underlying these traits in cotton (Gossypium spp.) have not been identified.ResultsWe identify four natural mutations in GoCEN-Dt associated with cluster fruiting (cl) and early maturity. The situ hybridization shows that GhCEN is preferentially expressed in cotton shoot apical meristems (SAM) of the main stem and axillary buds. Constitutive GhCEN-Dt overexpression suppresses the transition of the cotton vegetative apex to a reproductive shoot. Silencing GoCEN leads to early flowering and determinate growth, and in tetraploids causes the main stem to terminate in a floral bud, a novel phenotype that exemplifies co-adaptation of polyploid subgenomes and suggests new research and/or crop improvement approaches. Natural cl variations are enriched in cottons adapted to high latitudes with short frost-free periods, indicating that mutants of GoCEN have been strongly selected for early maturity.ConclusionWe show that the cotton gene GoCEN-Dt, a homolog of Antirrhinum CENTRORADIALIS, is responsible for determinate growth habit and cluster fruiting. Insight into the genetic control of branch and flower differentiation offers new approaches to develop early maturing cultivars of cotton and other crops with plant architecture appropriate for mechanical harvesting.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1518-8) contains supplementary material, which is available to authorized users.

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“…AGO1 stabilized miR168 posttranscriptional and the transcripts of AGO1 were regulated by miR168, which played an important role in plant development [ 29 ]. AGO1 and AGO10 interacted with miR172 and miR165/166 regulate the SAM and floral meristems development through targeting APETALA2 (AP2) and type III homeodomain-leucine zipper (HD-Zip) genes, respectively [ 28 , 30 , 31 , 32 ]. Cells in the SAM whether differentiated or not were regulated by HD-Zip transcription factors [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of DCL, AGO, and RDR Gene Families in Pepper (Capsicum Annuum L.)

Qin

Muhammad

et al. 2018

IJMS

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RNA silencing is an evolutionarily conserved mechanism that regulates variety of cellular processes in plants. Argonaute protein (AGO), Dicer-like protein (DCL) and RNA-dependent RNA polymerase (RDR) are critical components of RNA silencing. These efficient and indispensable components of the RNAi pathway have not been identified and characterized in pepper. In this study, we identified 12 CaAGO, 4 CaDCL and 6 CaRDR genes in pepper and compared them with those of Arabidopsis, tobacco, potato and tomato. Detailed phylogenetic analyses revealed that each CaAGO, CaDCL and CaRDR protein family were classified into four clades. The tissue specific expression and respond to abiotic or biotic stress were studied. The real-time quantitative polymerase chain reaction (PCR) results demonstrated that CaAGO2, CaAGO10b, CaDCL2 and CaDCL4 were upregulated with cucumber mosaic virus (CMV), potato virus Y (PVY) and tobacco mosaic virus (TMV) infections, whereas they showed difference expression patterns in response to abiotic stress. In addition, we found that many of the candidate genes were induced by phytohormones and H2O2 treatment. Our results provide useful information for further elucidation of gene silencing pathways and RNAi-mediated host immunity in pepper.

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AGO1 controls arabidopsis inflorescence architecture possibly by regulating TFL1 expression

Abstract: The results identify AGO1 as a repressor of TFL1 expression. Moreover, they reveal a novel role for AGO1 in inflorescence development, controlling the production of coflorescences. AGO1 seems to play this role through regulating TFL1 expression.

Cited by 20 publications

References 55 publications

Arabidopsis MAS2, an Essential Gene That Encodes a Homolog of Animal NF-κ B Activating Protein, Is Involved in 45S Ribosomal DNA Silencing

Arabidopsis MAS2, an Essential Gene That Encodes a Homolog of Animal NF-κ B Activating Protein, Is Involved in 45S Ribosomal DNA Silencing

Natural variation in a CENTRORADIALIS homolog contributed to cluster fruiting and early maturity in cotton

Genome-Wide Analysis of DCL, AGO, and RDR Gene Families in Pepper (Capsicum Annuum L.)

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