AgNPs Targeting the Drug Resistance Problem of Staphylococcus aureus: Susceptibility to Antibiotics and Efflux Effect

Abstract

The present work presents translational research with application of AgNPs targeting the global drug resistance problem. In vivo fieldwork was carried out with 400 breeding farm cows sick with a serous mastitis. Ex vivo results revealed that after cow treatment with LactobayTM (a mixture of antibiotic drugs) the susceptibility to 31 antibiotics of S. aureus isolates from cow breast secretion decreased by 25%, while after treatment with Argovit–CTM silver nanoparticles S. aureus susceptibility increased by 11%. The portion of isolates with an efflux effect leading to elimination of antibiotics from S. aureus after Lactobay-treatment resulted in a 15% increase, while Argovit-C-treatment led to a 17.5% decrease. The obtained results showed that mastitis treatments with Argovit-CTM AgNPs can partially restore the activity of antibiotics towards S. aureus and shorten the duration of mastitis treatment by 33%.

Silver Nanoparticles Targeting the Drug Resistance Problem of Streptococcus dysgalactiae: Susceptibility to Antibiotics and Efflux Effect

“…These results are similar to those observed in our previous work devoted to an analogous study for S. aureus and the treatment of cow mastitis with Lactobay and Argovit-C [ 8 ]. Figure 3 a represents the comparison of the changes in the susceptibility of S. aureus and S. dysgalactiae to antibiotics observed after treatment with Lactobay and Spectromast, respectively.…”

“…The present work is focused on S. dysgalactiae . The results for S. aureus were presented in [ 8 ]. Results for the other four types of bacteria will be presented elsewhere.…”

“…In our previous work, it was shown that the treatment of cows suffering from mastitis with Argovit-C AgNPs increased the activity of 31 antibiotics against Staphylococcus aureus by 11%, while antibiotic treatment decreased their activity by 25% [ 8 ]. Such effects were explained by the ability of Argovit-C AgNPs to decrease the portion of isolates with an efflux effect, while antibiotic treatment resulted in its increase [ 8 ].…”

“…In our previous work, it was shown that the treatment of cows suffering from mastitis with Argovit-C AgNPs increased the activity of 31 antibiotics against Staphylococcus aureus by 11%, while antibiotic treatment decreased their activity by 25% [ 8 ]. Such effects were explained by the ability of Argovit-C AgNPs to decrease the portion of isolates with an efflux effect, while antibiotic treatment resulted in its increase [ 8 ]. The present study presents the results which are the continuation of our previous article [ 8 ], and its purpose is to figure out if the same effects will be observed for Streptococcus species (Gram-positive cocci belonging to the Streptococcaceae family).…”

“…Such effects were explained by the ability of Argovit-C AgNPs to decrease the portion of isolates with an efflux effect, while antibiotic treatment resulted in its increase [ 8 ]. The present study presents the results which are the continuation of our previous article [ 8 ], and its purpose is to figure out if the same effects will be observed for Streptococcus species (Gram-positive cocci belonging to the Streptococcaceae family). The most common Streptococcus species that cause bovine mastitis are Streptococcus uberis and Streptococcus dysgalactiae [ 9 ].…”