AgNORs count and DNA ploidy in liver biopsies from patients with schistosomal liver cirrhosis and hepatocellular carcinoma

Attallah, Abdelfattah M.; Tabll, Ashraf A.; Nashar, Eman Mohamad El; El-Bakry, Kadry A.; Elsadany, Mohamed; Ibrahim, Tallat; El-Dosoky, Ibrahim

doi:10.1016/j.clinbiochem.2009.07.027

Cited by 8 publications

(8 citation statements)

References 28 publications

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“…Recently, we compared the results of DNA flow cytometry and AgNORs with the histopathological data. It was found that the lowest mean AgNOR count per nucleus was found in the normal liver (1.3± 0.9) and the highest count was (3.89± 0.827) in tumor lesion of HCC (Attallah et al, 2009b). A gradual increase in the AgNOR count per nucleus was noted as disease progressed from normal liver through liver cirrhosis to HCC tumor lesions.…”

Section: Agnor Count Reflects Dna Ploidy or Proliferative Activitymentioning

confidence: 74%

“…Moreover, FCM can identify cases with high cell proliferation (high S phase and G2/M), which are very susceptible to different mutagenic agents or chemicals, which results in neoplastic transformation. FCM can therefore be considered as a better ancillary technique in the assessment of liver cell dysplasia because it is objective and not able to misinterpretation or inter-observer discordance (Attallah et al, 2009b). In our previous study (El-Sayed et al, 2004), liver cell dysplasia was found in 60 % of patients with liver cirrhosis, small cell dysplasia in 38 % and large cell dysplasia in 62 %.…”

Section: Dna Ploidy and Liver Cell Dysplasia In Liver Biopsies From Pmentioning

confidence: 99%

“…Samples were excluded when CV exceeded 5%. Histograms showing only one G0/G1 peak was considered as diploid cells (normal) and those showing two distinct peaks were considered as aneuploid cells (Attallah et al, 2009b).…”

Section: Simple and Universally Applicable Methods For Staining Fixed mentioning

confidence: 99%

“…Staining was done in dark at room temperature for 30 min, then sections were immersed in 5% sodium thiosulphate for 5 min. Sections were washed in water, dehydrated in 95% and 100% ethanol, and mounted in permanent mounting media (Attallah et al, 2009b).…”

Section: Staining Of Nucleolar Organizer Regionsmentioning

confidence: 99%

See 3 more Smart Citations

The Use of Flow Cytometric DNA Ploidy Analysis of Liver Biopsies in Liver Cirrhosis and Hepatocellular Carcinoma

Tabll¹,

Ismail²

2011

Liver Biopsy

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Section: Agnor Count Reflects Dna Ploidy or Proliferative Activitymentioning

confidence: 74%

Section: Dna Ploidy and Liver Cell Dysplasia In Liver Biopsies From Pmentioning

confidence: 99%

Section: Simple and Universally Applicable Methods For Staining Fixed mentioning

confidence: 99%

Section: Staining Of Nucleolar Organizer Regionsmentioning

confidence: 99%

See 2 more Smart Citations

The Use of Flow Cytometric DNA Ploidy Analysis of Liver Biopsies in Liver Cirrhosis and Hepatocellular Carcinoma

Tabll¹,

Ismail²

2011

Liver Biopsy

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“…Previously, nucleolar staining by AgNORs was considered to be a prognostic marker of malignancy (25). Moreover, the value of AgNORs as proliferation markers has been reported in various forms of cancer, such as breast, ovarian, cervical, prostate, hepatocellular, papillary thyroid, gastric and bladder cancer (25–31).…”

Section: Overexpression Of Eif6 In Human Carcinomamentioning

confidence: 99%

The role of eukaryotic translation initiation factor 6 in tumors

Zhu

Chen

et al. 2017

Oncology Letters

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Eukaryotic translation initiation factor 6 (eIF6) affects the maturation of 60S ribosomal subunits. Found in yeast and mammalian cells, eIF6 is primarily located in the cytoplasm of mammalian cells. Emerging evidence has demonstrated that the dysregulated expression of eIF6 is important in several types of human cancer, including head and neck carcinoma, colorectal cancer, non-small cell lung cancer and ovarian serous adenocarcinoma. However, the molecular mechanisms by which eIF6 functions during tumor formation and progression remain elusive. The present review focuses on recent progress in terms of the mechanisms and functions of eIF6 in human tumorigenesis or cancer cell lines, along with the signal transduction pathways in which this novel translation initiation factor may participate. Oncogenic Ras activates Notch-1 and promotes transcription of eIF6 via a recombining binding protein suppressor of Hairless-dependent mechanism. In addition, overexpression of eIF6 results in aberrant activation of the Wnt/β-catenin signaling pathway. Similarly, overexpressed eIF6 regulates its downstream modulator, cell division control protein 42, which in turn affects oncogenesis. Finally, the potential of eIF6 as a biomarker for diagnosis of cancer is also discussed in the present review.

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Nucleus, Nuclear Structure, and Nuclear Functions: Pathogenesis of Nuclear Abnormalities in Cancer

Zimmermann¹

2016

Tumors and Tumor-Like Lesions of the Hepatobiliary Tract

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AgNORs count and DNA ploidy in liver biopsies from patients with schistosomal liver cirrhosis and hepatocellular carcinoma

Cited by 8 publications

References 28 publications

The Use of Flow Cytometric DNA Ploidy Analysis of Liver Biopsies in Liver Cirrhosis and Hepatocellular Carcinoma

The Use of Flow Cytometric DNA Ploidy Analysis of Liver Biopsies in Liver Cirrhosis and Hepatocellular Carcinoma

The role of eukaryotic translation initiation factor 6 in tumors

Nucleus, Nuclear Structure, and Nuclear Functions: Pathogenesis of Nuclear Abnormalities in Cancer

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