AgNOR numbers in rat pituitary corticotrophs following adrenalectomy or corticotrophin releasing factor administration

Peebles, Susan E.; McNicol, Anne Marie

doi:10.1007/bf02899083

Cited by 10 publications

(6 citation statements)

References 12 publications

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“…The NOR associated proteins are known to include RNA polymerase 1, C23 protein (nucleolin), and B23 protein; their exact structure and function remain uncertain (1 8). However, alterations in the number and configuration of AgNORs have been demonstrated in numerous types of human neoplasms (18,19,20) and also in human trophoblastic tissues (61), rat tumors (62, 63), and in the rat pituitary following hormone stimulation (50). The aim of the current work is to study the AgNORs in routine histological sections of myocardial lesions of doxorubicin-treated rats in order to characterize the nuclear changes induced by doxorubicin in myocytes.…”

Section: Introductionmentioning

confidence: 99%

Nucleolar Organizer Regions in Cardiac Lesions Induced by Doxorubicin

et al. 1991

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ABSTRAC~The use of the argyrophilic (Ag) staining technique for nucleolar organizer regions (NORs) revealed nuclear changes in myocytes of the left atrium of 10 rats treated twice a week for 6 weeks with doxorubicin (1 mg/ kg body weight) iv and sacrificed after 6 weeks without treatment. The changes were easily detected qualitatively and further assessed by quantification. Cardiac myocytes of doxorubicin-treated rats had larger nuclei and/or a larger quantity of AgNORs that were either dispersed in a number of small dots or clustered in rounded, rod-shaped, or tortuous large structures. AgNOR alterations may reflect a defect of nucleolar association leading to an impairment ofprotein synthesis that could be involved in doxorubicin cardiotoxicity.

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Section: Introductionmentioning

confidence: 99%

Nucleolar Organizer Regions in Cardiac Lesions Induced by Doxorubicin

et al. 1991

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“…In rat pituitary glands the number of Ag-NORs in anterior lobe corticotrophs was increased following bilateral adrenalectomy [23]. These studies indicated that the number of AgNORs changed reflecting increased ribosomal gene transcription in corticotrophs or adrenalectomized animals, because both rough endoplasmic reticulum and pro-opiomelanocortin mRNA levels were increased in these cells [15,17].…”

Section: Methodsmentioning

confidence: 83%

Age-Related Changes of the Argyrophilic Nucleolar Organizer Regions in Mouse Neocortical Neurons.

Fushiki

Kinoshita

Tsutsumi

et al. 1995

Acta Histochem. Cytochem

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“…AgNOR values obtained for pituitary adenomas (Table 1; Figure le) were difficult to interpret for two reasons. Firstly, the dependency of AgNOR number on endocrine stimulation has been reported after growth hormone application in vitro (de Capao et al, 1985) and in rat corticotrophs following bilateral adrenalectomy (Peebles & McNicol, 1989). Thus, one would expect a relationship between the AgNOR number and hormone production by the adenoma.…”

Section: Discussionmentioning

confidence: 94%

Cell proliferation in intracranial tumours: selective silver staining of nucleolar organizer regions (AgNORs). Application to surgical and experimental neuro‐oncology

Plate

Rüschoff

Mennel

1991

Neuropathology Appl Neurobio

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A novel tool in diagnostic and experimental pathology, the AgNOR-technique, which consists of visualization of ribosomal gene activity by selective silver staining, was applied to 144 cytological specimens of human tumours of the nervous system. The number of silver-stained nucleolar organizer regions (AgNORs) was correlated with the biological behaviour of the tumours investigated; low AgNOR number were observed in benign neoplasms such as meningiomas and schwannomas and higher AgNOR numbers in glioblastomas and metastases. The mean AgNOR number per cell was 3.15 in astrocytomas, 4.5 in anaplastic astrocytomas and 5.86 in glioblastoma multiforme. Benign and malignant lesions showed different distribution patterns of AgNORs, with few but centrally located AgNORs in benign, and multiple but scattered AgNORs in malignant tumours. AgNOR number per cell and AgNOR area revealed an inverse relationship (correlation coefficient -0.15, linear regression). In addition to the human tumours, two N-nitroso-N-ethyl-urea (NEU) induced tumors in BD-IX rats a mixed glioma (G-XIII) and a malignant schwannoma (N-XII), were investigated. Twelve G-XIII gliomas revealed homogenous AgNOR-counts (standard error of the mean less than 10%), with absolute values between the values obtained for human glioblastomas and metastases. Seven N-XIII subcutaneously transplanted schwannomas revealed higher AgNOR values than human schwannomas, but lower than experimental gliomas. It is concluded that the AgNOR method, as a technique for visualization of ribosomal gene activity, is valuable for assessing proliferative activity and malignancy in both diagnostic and experimental neuropathology.

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AgNOR numbers in rat pituitary corticotrophs following adrenalectomy or corticotrophin releasing factor administration

Cited by 10 publications

References 12 publications

Nucleolar Organizer Regions in Cardiac Lesions Induced by Doxorubicin

Nucleolar Organizer Regions in Cardiac Lesions Induced by Doxorubicin

Age-Related Changes of the Argyrophilic Nucleolar Organizer Regions in Mouse Neocortical Neurons.

Cell proliferation in intracranial tumours: selective silver staining of nucleolar organizer regions (AgNORs). Application to surgical and experimental neuro‐oncology

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