Aging Studies in Drosophila Melanogaster

Sun, Yong; Yolitz, Jason; Wang, Cecilia; Spangler, Edward L.; Zhan, Ming; Zou, Sige

doi:10.1007/978-1-62703-556-9_7

Cited by 64 publications

(59 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In Drosophila, foodborne paraquat exposure is the standard assay for testing oxidative stress resistance (Rzezniczak et al 2011;Sun et al 2013) and many fly studies successfully used this single agent (e.g., Alic et al 2011;Barnes et al 2014;Lin et al 2014). Following this protocol, we observed increased paraquat resistance in all tested mutants deficient in AKH signaling.…”

Section: Roles Of Akh Signaling In Oxidative Stress Resistancementioning

confidence: 89%

“…However, the AKH titer positively correlated with oxidative stress induced by bacterial toxin in Leptinotarsa decemlineata , and by paraquat in P. apterus (Večeřa et al 2007) and L. decemlineata ). Moreover, co-injection of AKH alleviated the effect of paraquat by enhancing the antioxidative capacity in the firebug (Večeřa et al 2007), suggesting a protective role of AKH during oxidative stress response.In Drosophila, foodborne paraquat exposure is the standard assay for testing oxidative stress resistance (Rzezniczak et al 2011;Sun et al 2013) and many fly studies successfully used this single agent (e.g., Alic et al 2011;Barnes et al 2014;Lin et al 2014). Following this protocol, we observed increased paraquat resistance in all tested mutants deficient in AKH signaling.…”

mentioning

confidence: 89%

See 1 more Smart Citation

Energy Homeostasis Control in Drosophila Adipokinetic Hormone Mutants

et al. 2015

View full text Add to dashboard Cite

Maintenance of biological functions under negative energy balance depends on mobilization of storage lipids and carbohydrates in animals. In mammals, glucagon and glucocorticoid signaling mobilizes energy reserves, whereas adipokinetic hormones (AKHs) play a homologous role in insects. Numerous studies based on AKH injections and correlative studies in a broad range of insect species established the view that AKH acts as master regulator of energy mobilization during development, reproduction, and stress. In contrast to AKH, the second peptide, which is processed from the Akh encoded prohormone [termed "adipokinetic hormone precursor-related peptide" (APRP)] is functionally orphan. APRP is discussed as ecdysiotropic hormone or as scaffold peptide during AKH prohormone processing. However, as in the case of AKH, final evidence for APRP functions requires genetic mutant analysis. Here we employed CRISPR/Cas9-mediated genome engineering to create AKH and AKH plus APRP-specific mutants in the model insect Drosophila melanogaster. Lack of APRP did not affect any of the tested steroid-dependent processes. Similarly, Drosophila AKH signaling is dispensable for ontogenesis, locomotion, oogenesis, and homeostasis of lipid or carbohydrate storage until up to the end of metamorphosis. During adulthood, however, AKH regulates body fat content and the hemolymph sugar level as well as nutritional and oxidative stress responses. Finally, we provide evidence for a negative autoregulatory loop in Akh gene regulation.KEYWORDS Drosophila; adipokinetic hormone; adipokinetic hormone precursor-related peptide; energy homeostasis; stress resistance E NERGY homeostasis requires continuous compensation for fluctuations in the energy expenditure and availability of food resources. Organisms thus build up reserves under positive energy balance and catabolize them when the balance turns negative to retain stable levels of circulating energy fuel. Insulin signaling induces the uptake of excessive circulating sugars, thus promoting reserve accumulation (reviewed, e.g., in Saltiel and Kahn 2001; Cohen 2006), whereas energy mobilization is under the control of glucagon and glucocorticoid signaling in mammals (reviewed, e.g., in Rui 2014;Charron and Vuguin 2015) and adipokinetic hormone (AKH) signaling in insects (reviewed, e.g., in Van der Horst 2003;Lorenz and Gäde 2009;Bednářová et al. 2013a). Consistent with their fundamental physiological function in energy mobilization, AKHs are found not only in insects, but are common in Protostomia, where they have been identified both in Ecdyszoa (in Arthropoda, Tardigrada, and Priapulida) and Lophotrochozoa (in Mollusca, Rotifera, and Annelida) (Gäde 2009;Hauser and Grimmelikhuijzen 2014). Nevertheless, physiological functions of AKHs have been studied mainly in Arthropoda. Similar to mammals, also insects store lipids in the form of triacylglycerides (TGs) and as carbohydrates in the form of glycogen. The main storage organ for lipid and glycogen in insects is the fat body, which can thus b...

show abstract

Section: Roles Of Akh Signaling In Oxidative Stress Resistancementioning

confidence: 89%

mentioning

confidence: 89%

Energy Homeostasis Control in Drosophila Adipokinetic Hormone Mutants

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The comparatively short lifespan of Drosophila with age-related changes in metabolism and physiology makes Drosophila an excellent model for aging and circadian studies (He and Jasper, 2014, Sun et al, 2013). Aging related issues in Drosophila surface by 20-30 days of age, analogous to the middle age in humans (Dambroise et al, 2016, Horiuchi et al, 2003, Vaccaro et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Aging and circadian dysfunction increase alcohol sensitivity and exacerbate mortality in Drosophila melanogaster

Nobrega

Mellers

Lyons

2017

Experimental Gerontology

View full text Add to dashboard Cite

Alcohol abuse is a rising problem in middle-aged and older individuals resulting in serious health, family and economic consequences. Effective treatment necessitates the identification of factors influencing alcohol toxicity with aging. We investigated the interaction between aging, alcohol toxicity and circadian function using Drosophila as a model system. We found as wild type flies age, sensitivity to alcohol increases and circadian regulation of alcohol-induced behaviors weakens. Decreased circadian modulation is correlated with significantly greater alcohol sensitivity during the subjective day. The circadian clock modulates alcohol-induced mortality in younger flies with increased mortality following alcohol exposure at night. Older flies exhibit significantly longer recovery times following alcohol-induced sedation and increased mortality following binge-like or chronic alcohol exposure. Flies rendered arrhythmic either genetically or environmentally exhibit significantly increased alcohol sensitivity, longer recovery times and increased mortality. We hypothesize that the circadian clock phase specifically buffers behavioral and cellular alcohol sensitivity with this protection diminishing as the circadian clock weakens with age.

show abstract

“…Some insect species have been used for laboratory experiments for several decades (e.g., silkworms, honey bees, and other species [54,203,204]), especially the fruit flies (Drosophila spp.) [205][206][207].…”

Section: Insects For Laboratory Researchmentioning

confidence: 99%

Insects: The Disregarded Domestication Histories

Lecocq¹

2019

Animal Domestication

View full text Add to dashboard Cite

Domestication has irrevocably impacted human evolution. The domestication process/ pathways have been the focus of abundant research for plants and vertebrates. Advances in genetics and archeology have allowed tremendous progresses in the understanding of domestication for these organisms. In contrast, insects' domestication has comparatively received far less attention to date. Yet, insects are the most common animal group on Earth and provide many valuable ecosystem services to humans. Therefore, the aims of this chapter are (i) to provide an overview of main ancient and recent insect domestication histories and (ii) to reread them by the light of the domestication process, pathways, triggers, and consequences observed in other animal species. Some of the considered species (i.e., silkworm and honey bee) have been chosen because they are among the few insects commonly acknowledged as domesticated, while others allow illustrating alternative domestication patterns. The overview of current literature shows similar humandirected pathway and domestication syndrome (e.g., increased tameness, decreased aggressiveness, modified reproduction) between several insect species.

show abstract

Aging Studies in Drosophila Melanogaster

Cited by 64 publications

References 61 publications

Energy Homeostasis Control in Drosophila Adipokinetic Hormone Mutants

Energy Homeostasis Control in Drosophila Adipokinetic Hormone Mutants

Aging and circadian dysfunction increase alcohol sensitivity and exacerbate mortality in Drosophila melanogaster

Insects: The Disregarded Domestication Histories

Contact Info

Product

Resources

About