Aging of Murine Mesenchymal Stem Cells

Fehrer, Christine

doi:10.1196/annals.1354.030

Cited by 36 publications

(34 citation statements)

References 18 publications

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“…The molecular mechanisms of this "Hayflick limit" are yet unknown. Certainly, molecular profiles and functional features of MSC are affected by this process of cellular aging [49][50][51]. Cell density of cultures seems to be crucial, too.…”

Section: In Vitro Cultivation (Passage Density and Cryopreservation)mentioning

confidence: 98%

Mesenchymal Stem Cell Preparations—Comparing Apples and Oranges

2007

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Mesenchymal stem cells (MSC) represent a type of adult stem cells that can easily be isolated from various tissues and expanded in vitro. Past reports on their pluripotency and possible clinical applications have raised hopes and interest in MSC. Multiple designations have been given to different MSC preparations. So far MSC are poorly defined by a combination of physical, phenotypical and functional properties. As MSC could be derived from different tissues as starting material, by diverse isolation protocols, cultured and expanded in different media and conditions, the MSC preparations from different laboratories are highly heterogeneous. All of these variables might have implications (1) on the selection of cell types and the composition of heterogeneous subpopulations; (2) they can selectively favor expansion of different cell populations with totally different potentials; or (3) they might alter the long term fate of adult stem cells upon in vitro culture. The recent controversy on the multilineage differentiation potentials of some specific MSC preparations might be attributed to this lack of common standards. More precise molecular and cellular markers to define subsets of MSC and to standardize the protocols for expansion of MSC are urgently needed.

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Section: In Vitro Cultivation (Passage Density and Cryopreservation)mentioning

confidence: 98%

Mesenchymal Stem Cell Preparations—Comparing Apples and Oranges

2007

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“…17,18 It has been suggested that MSCs from different mouse strains (SAMP6, SAMR1, C57BL/6) with different life expectancies may be intrinsically similar in their in vitro proliferation and differentiation capacity, despite exhibiting dramatically different in vivo differentiation and proliferation capacity due to the influence of heterogeneous microenvironments. 19 Furthermore, age is associated with changes in the ECM that have been linked to multiple pathologies (reviewed in ref. 20 ), including cancer.…”

Section: Introductionmentioning

confidence: 99%

Age associated communication between cells and matrix: a potential impact on stem cell-based tissue regeneration strategies

Lynch

Pei²

2014

Organogenesis

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A recent paper demonstrated that decellularized extracellular matrix (DECM) deposited by synovium-derived stem cells (SDSCs), especially from fetal donors, could rejuvenate human adult SDSCs in both proliferation and chondrogenic potential, in which expanded cells and corresponding culture substrate (such as DECM) were found to share a mutual reaction in both elasticity and protein profiles (see ref.

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“…As a matter of fact, molecular pro fi les and functional features of MSC are signi fi cantly affected by this process of cellular aging (DiGirolamo et al 1999 ;Fehrer et al 2006 ;Javazon et al 2004 ;Wagner et al 2008Wagner et al , 2009 . Cell density of cultures seems to be crucial, too.…”

Section: In Vitro Cultivation (Passage Density and Cryopreservation)mentioning

confidence: 99%

Mesenchymal Stem Cells – An Oversimplified Nomenclature for Extremely Heterogeneous Progenitors

Wuchter

Wagner²,

2013

Regenerative Medicine

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Mesenchymal stem cells (MSC) are plastic-adherent fi broblast-like cells that can readily be isolated from various tissues and expanded in vitro. Per de fi nitionem , they are able to differentiate into bone, cartilage and adipose tissue. In the last 15 years, a huge number of different preparative protocols have been developed to yield MSC-like cell lines from starting materials as diverse as bone marrow, fat tissue, umbilical cord blood and peripheral blood. However, these protocols as well as the resulting cell populations are heterogeneous. Furthermore, the composition of the cell products and their differentiation potential changes in the course of long-term culture expansion. There is an urgent need for the development of molecular markers and universal criteria for quality control of the starting cell populations as well as for the cell products after expansion. Nevertheless, MSC have already found their way into a huge number of clinical studies addressing a broad variety of diseases. Even though there is no convincing evidence that MSC are involved in the process of tissue repair by transdifferentiation, they probably contribute to the repair process by immunomodulatory effects and interaction with other cell types.

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Aging of Murine Mesenchymal Stem Cells

Cited by 36 publications

References 18 publications

Mesenchymal Stem Cell Preparations—Comparing Apples and Oranges

Mesenchymal Stem Cell Preparations—Comparing Apples and Oranges

Age associated communication between cells and matrix: a potential impact on stem cell-based tissue regeneration strategies

Mesenchymal Stem Cells – An Oversimplified Nomenclature for Extremely Heterogeneous Progenitors

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