Aging of
            <i>Escherichia coli</i>

Clifton, C. E.

doi:10.1128/jb.92.4.905-912.1966

Cited by 11 publications

(2 citation statements)

References 15 publications

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“…Degradation of RNA and release of the degradation products have been reported to occur in populations of starved or stressed bacteria (4,6,8,18,25,29). In contrast, Vibrio sp.…”

Section: Discussionmentioning

confidence: 99%

In situ analysis of nucleic acids in cold-induced nonculturable Vibrio vulnificus

Weichart

McDougald

Jacobs

et al. 1997

Appl Environ Microbiol

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Low-temperature-induced nonculturable cells of the human pathogenic bacterium Vibrio vulnificus retained significant amounts of nucleic acids for more than 5 months. Upon permeabilization of fixed cells, however, an increasing number of cold-incubated cells released the nucleic acids. This indicates substantial degradation of DNA and RNA in nonculturable cells prior to fixation. Treatment of permeabilized cells with DNase and RNase allowed differential staining of DNA and RNA with the nucleic acid dye 4,6-diamidino-2-phenylindole (DAPI). Epifluorescence microscopy revealed that the cold-induced nonculturable populations of V. vulnificus are highly heterogeneous with regard to their nucleic acid content. The fraction of nonculturable cells which maintained DNA and RNA structures decreased gradually during cold incubation. After 5 months at 5°C, less than 0.05% of the cells could be observed to retain DNA and RNA. In parallel with the loss of nucleic acids, an increase in the concentrations of UV-absorbing material in the culture supernatants was observed in nonculturable-cell suspensions. It is hypothesized that there are two phases of the formation of nonculturable cells of V. vulnificus: the first involves a loss of culturability with maintenance of cellular integrity and intact RNA and DNA (and thus possibly viability), and the second is typified by a gradual degradation of nucleic acids, the products of which partly remain inside the cells and partly diffuse into the extracellular space. A small number of nonculturable cells, however, retain DNA and RNA, and thus may be viable despite having reduced culturability.

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“…Degradation of RNA and release of the degradation products have been reported to occur in populations of starved or stressed bacteria (4,6,8,18,25,29). In contrast, Vibrio sp.…”

Section: Discussionmentioning

confidence: 99%

In situ analysis of nucleic acids in cold-induced nonculturable Vibrio vulnificus

Weichart

McDougald

Jacobs

et al. 1997

Appl Environ Microbiol

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“…The longevity of S. enteritidis cells starved in dilute suspension was not anticipated since most Enterobacteriaceae do not survive beyond a few days in a nutrient-free environment (2,5,17,25,29,30). Ensign (6) compared the half-life survival times for starving vegetative cells of several bacteria.…”

Section: Discussionmentioning

confidence: 99%

Starvation survival of Salmonella enteritidis

Druilhet¹,

Sobek²

1976

J Bacteriol

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Washed cells of Salmonella enteritidis harvested from a defined medium during logarithmic growth were subjected to starvation in pH 7 phosphate buffer at 37 C. Viability was measured by slide cultures and plate counts. The survival of cell suspensions equivalent to 1 to 10 mg (dry wt)/ml was influenced by cryptic growth. The rate of cryptic growth, assessed by plate counts, increased with cell density and could not be alleviated by starvation with dialysis. Dialysis of the starving culture did retard the onset of cryptic growth but did not eliminate it, indicating that the major substrates for regrowth were relatively large cellular components. In phosphate buffer, 6.7 homologous heat-killed cells allowed for the doubling of one S. enteritidis cell. Cryptic growth was not observed when cells were starved on the surface of membrane filters or in suspensions equivalent to 20 jig (dry wt)/ml (10' cells/ml). Similar half-life survival times were calculated for both these populations, but the shape of their survival curves differed significantly. These differences were attributed to stress factors encountered during cell preparation and during starvation. The half-life survival time of S. enteritidis on July 15, 2020 by guest

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Physiologische Untersuchungen zur Atmung von Vibro luminosus Beijerinck

K�ssler¹

1968

Archiv. Mikrobiol.

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Aging of Escherichia coli

Cited by 11 publications

References 15 publications

In situ analysis of nucleic acids in cold-induced nonculturable Vibrio vulnificus

In situ analysis of nucleic acids in cold-induced nonculturable Vibrio vulnificus

Starvation survival of Salmonella enteritidis

Physiologische Untersuchungen zur Atmung von Vibro luminosus Beijerinck

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