Aging in Connective Tissue: A Dynamic Process*

McGavack, Thomas H.; Kao, Kung-Ying Tang

doi:10.1111/j.1532-5415.1963.tb02190.x

Cited by 10 publications

(7 citation statements)

References 38 publications

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“…While elastin is a relatively minor component of the skin’s extracellular matrix, it plays a primary role in skin elasticity (Tzaphlidou, 2004) and tends to become damaged by solar exposure. However, elastin levels have been found to decrease with age (McGavack & Kao, 1963). While all animals appeared similar in age, we had no specific history for any of the animals in our tests.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the effect of formalin fixation on skin specimens in dogs and cats

Miller

Dark

2014

PeerJ

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Skin and subcutaneous tissues are the origin of most common neoplasms affecting dogs, accounting for approximately one third of all tumors encountered in the species. Surgical excision is frequently the best chance for a cure; determining factors influencing the success of excision are vital for surgical management of cases. This work examined the shrinkage of skin of various lengths from three sites in formalin for both dogs and cats. Tissues were measured on the animal (initial measurement), at the time of excision (post-removal), and after formalin fixation (post-fixation). While shrinkage after tissue removal was found in samples from the thorax, abdomen, and rear leg in dogs and from the rear leg in cats, no significant shrinkage due to formalin fixation was detected in any sample except for the thoracic samples from the dog. Therefore, when determining where to make incisions to effect a surgical cure, initial measurements should take into account tissue shrinkage effects.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the effect of formalin fixation on skin specimens in dogs and cats

Miller

Dark

2014

PeerJ

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“…In vitro experiments have shown, however, that the capacity of the uterus to synthesize collagen is a n inverse function of age (Kao et al, 1962;McGavack and Kao, 1963), while the nature and composition of collagen appear to be modified with time (Sobel, 1967). The amount of soluble collagen decreases as a percentage of the total with age, while the insoluble collagen increases (Kao and McGavack, 1959;McGavack and Kao, 1963), rendering the collagen more resistant to the action of bacterial collagenase (Woessner, 1963) and cathepsin (Schaub, 1965). Uterine collagen from women (Woessner, 1963) and golden hamsters (14-22 months) (Rahima and Soderwall, 1977) also shows increased levels of hydroxyproline with age, which may affect the degree of cross-linking and, hence, the susceptibility of the collagen to the action of collagenases.…”

Section: Histological and Related Features Of The Aged Uterusmentioning

confidence: 99%

Characteristics of uterine aging

Mulholland

Jones

1993

Microscopy Res & Technique

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Uterine aging is in part responsible for a decline in fecundity which begins in middle age in most mammals. Characteristics of uteri from a variety of animals in middle age and old age are reviewed and the factors which may be responsible for this decline discussed. These include age-related changes in the hypothalamus, pituitary, and ovaries; loss of number or function of steroid hormone receptors; morphological changes in the uterine epithelium; the accumulation of collagen fibrils in the uterine stroma; and loss or impairment of the decidual response. The ultrastructural morphology of uterine epithelial, stromal, and decidual tissue from 4 month old and 18 month old Fischer 344 rats is presented and compared.

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“…Both an increase and a change in the configuration of uterine collagen have been reported in the ageing rat (Burack, Wolfe, Lansing & Wright, 1941; Kao & McGavack, 1959;Finn, Fitch & Harkness, 1963;Schaub, 1964/65). A decrease in the activity of collagenase and other proteases capable of degrading collagen was also reported (McGavack & Kao, 1963;Schaub, /65, 1965.…”

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confidence: 95%

“…The tendency for acid-soluble and total collagen content to increase in the ageing female rabbit is in agreement with earlier findings in the mouse and rat (Kao &McGavack, 1959;Finn etal, 1963;Schaub, 1964/65). Table 2 alkaline-and acid-soluble uterine collagen content of post-partum and Collagen structural changes occur with increased age; this reduces the ability of collagenase to degrade collagen fibres (McGavack & Kao, 1963;Schaub, /65, 1965. This structural change in collagen together with the reduction in collagenase could contribute to the higher uterine collagen content of old does.…”

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confidence: 99%

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Uterine Collagenase and Collagen in Young and Ageing Rabbits

Maurer¹,

Foote²

1972

Reproduction

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Kao, 1963; Schaub, /65, 1965.This investigation was conducted to examine changes in uterine collagen and collagenase activity in ageing female rabbits with declining reproductive ability.Fifteen parous does were divided into three groups of five, averaging 34, 167 and 204 weeks of age and averaging one, nine and twenty-four previous litters, respectively. The does were killed and exsanguinated between 66 and 75 hr after parturition. In preliminary trials, this was found to be the time of highest collagenase activity. Woessner (1962) found the peak of collagen degradation in the involuting rat uterus to be between 48 and 72 hr after parturition. The uteri were removed and excess fluids were squeezed out before weighing. To measure collagenase content, each horn was cut longitudinally along the mesenteric and antimesenteric region and a 5-g (wet weight) sample was removed, minced and homogenized for 10 min with 30 ml 0-01 M-calcium acetate (according to Kit 08001 -8300, Mann Research Laboratories). The homo¬ genate was centrifuged at 15,000 g for 15 min and the supernatant was decanted and filtered into a measuring cylinder. Collagenase activity was determined according to the method of Wünsch & Heidrich (1963) using a synthetic chromophore, acyl-pentapeptide, 4-phenyl-azo-benzyloxycarbonyl-L-prolyl-Lleucyl-glycyl-L-prolyl-D-arginine dihydrate (Mann Research Laboratories). The assay was conducted at 37°C and a pH of 7-1. These conditions were chosen

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Aging in Connective Tissue: A Dynamic Process*

Cited by 10 publications

References 38 publications

Evaluation of the effect of formalin fixation on skin specimens in dogs and cats

Evaluation of the effect of formalin fixation on skin specimens in dogs and cats

Characteristics of uterine aging

Uterine Collagenase and Collagen in Young and Ageing Rabbits

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