Aging in brown fat: Antioxidant defenses and oxidative stress

López-Torres, Mónica; Perez-Ocampo, R.; Barja, Gustavo

doi:10.1016/0047-6374(91)90079-f

Cited by 7 publications

(8 citation statements)

References 29 publications

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“…Another component of the anti−/pro-oxidant system as it may relate to WNS involves the extreme fluctuations in oxygen consumption of bats while they periodically transition from deep torpor to euthermia [65]. During arousals, the rapid rates of oxygen consumption in brown adipose tissue (BAT) promote the generation of reactive oxygen species [66], which easily oxidize lipid molecules [67].…”

Section: Discussionmentioning

confidence: 99%

Hibernating Little Brown Myotis (Myotis lucifugus) Show Variable Immunological Responses to White-Nose Syndrome

et al. 2013

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White-nose syndrome (WNS) is an emerging infectious disease devastating hibernating North American bat populations that is caused by the psychrophilic fungus Geomyces destructans. Previous histopathological analysis demonstrated little evidence of inflammatory responses in infected bats, however few studies have compared other aspects of immune function between WNS-affected and unaffected bats. We collected bats from confirmed WNS-affected and unaffected sites during the winter of 2008–2009 and compared estimates of their circulating levels of total leukocytes, total immunoglobulins, cytokines and total antioxidants. Bats from affected and unaffected sites did not differ in their total circulating immunoglobulin levels, but significantly higher leukocyte counts were observed in bats from affected sites and particularly in affected bats with elevated body temperatures (above 20°C). Bats from WNS-affected sites exhibited significantly lower antioxidant activity and levels of interleukin-4 (IL-4), a cytokine that induces T cell differentiation. Within affected sites only, bats exhibiting visible fungal infections had significantly lower antioxidant activity and levels of IL-4 compared to bats without visible fungal infections. Overall, bats hibernating in WNS-affected sites showed immunological changes that may be evident of attempted defense against G. destructans. Observed changes, specifically elevated circulating leukocytes, may also be related to the documented changes in thermoregulatory behaviors of affected bats (i.e. increased frequencies in arousal from torpor). Alterations in immune function may reflect expensive energetic costs associated with these processes and intrinsic qualities of the immunocapability of hibernating bats to clear fungal infections. Additionally, lowered antioxidant activity indicates a possible imbalance in the pro- versus antioxidant system, may reflect oxidative tissue damage, and should be investigated as a contributor to WNS-associated morbidity and mortality.

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Section: Discussionmentioning

confidence: 99%

Hibernating Little Brown Myotis (Myotis lucifugus) Show Variable Immunological Responses to White-Nose Syndrome

et al. 2013

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“…For example, Lopez‐Torres et al . have reported a significant increase in the antioxidant enzymes in the BAT of 3–9‐month‐old rats. However, direct evidence of oxidative stress impairing brown adipocyte function has not been reported.…”

Section: Discussionmentioning

confidence: 96%

“…There is a large body of research suggesting that there is a causal relationship between oxidative stress and BAT dysfunction. For example, Lopez-Torres et al [15] have reported a significant increase in the antioxidant enzymes in the BAT of 3-9-monthold rats. However, direct evidence of oxidative stress impairing brown adipocyte function has not been reported.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have identified several major factors that contribute to the aging‐related impairment of BAT: (a) defects at the stem and/or progenitor cell level, (b) mitochondrial dysfunction, (c) impaired ability to sense sympathetic tone, and (d) endocrine changes that involve hormones and inflammatory cytokines . In general, mitochondrial dysfunction is associated with a decrease in fuel oxidation and an increase in the production of reactive oxygen species (ROS) . This accumulation of ROS leads to cellular oxidative stress, resulting in direct or indirect macromolecular damage to proteins, nucleic acids, and lipids that further exacerbates mitochondrial dysfunction .…”

Section: Introductionmentioning

confidence: 99%

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Age‐induced oxidative stress impairs adipogenesis and thermogenesis in brown fat

et al. 2019

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It is well‐established that the mass and function of human brown adipose tissue (BAT) declines with age. A key factor involved in age‐related impairment of BAT is oxidative stress; however, there is a paucity of studies to date that have explored this relationship. Here, we characterized the age‐related molecular and functional alterations in BAT in vivo in mice of different ages, and treated human brown adipocytes with H2O2 to dissect the direct effect of oxidative stress in vitro. We further explored the structural and functional changes in BAT in an oxidative stress‐induced mouse model of aging. We found that the progressive deterioration of BAT was linked to oxidative stress, and observed that the adipogenesis and thermogenic program were significantly impaired upon H2O2 treatment in vitro. Moreover, antioxidant supplementation (e.g., vitamin E) attenuated oxidative stress and rescued BAT activity decline, suggesting that age‐related injury in BAT function can be partly alleviated by antioxidant treatment. Finally, we found that oxidative stress‐induced BAT dysfunction is linked to the enhancement of autophagy. These results point to oxidative stress as being an important factor in age‐dependent functional impairment of BAT.

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“…Changes in absorbance were read on a SPECTRAmax Plus microplate spectrophotometer (Molecular Devices Corp. Sunnyvale, CA, USA). Additionally, the GSH-Px was assayed at 25°C as described by Paglia and Valentine (1967) and Lopez-Torres et al (1991), wherein NADPH oxidation was followed by spectrophotometry at 340 nm in the presence of reduced glutathione (GSH) and hydrogen peroxide (H 2 O 2 ). To correct spontaneous reactions in the absence of enzyme, blanks were run without samples and then subtracted from the assay values.…”

Section: Methodsmentioning

confidence: 99%

Effect of γ-Aminobutyric Acid (GABA) Producing Bacteria on <italic>In vitro</italic> Rumen Fermentation, Biogenic Amine Production and Anti-oxidation Using Corn Meal as Substrate

Mamuad

Kim

et al. 2013

Asian Australas. J. Anim. Sci

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The effects and significance of γ-amino butyric acid (GABA) producing bacteria (GPB) on in vitro rumen fermentation and reduction of biogenic amines (histamine, methylamine, ethylamine, and tyramine) using corn meal as a substrate were determined. Ruminal samples collected from ruminally fistulated Holstein cows served as inoculum and corn was used as substrate at 2% dry matter (DM). Different inclusion rates of GPB and GABA were evaluated. After incubation, addition of GPB had no significant effect on in vitro fermentation pH and total gas production, but significantly increased the ammonia nitrogen (NH3-N) concentration and reduced the total biogenic amines production (p<0.05). Furthermore, antioxidation activity was improved as indicated by the significantly higher concentration of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) among treated samples when compared to the control (p<0.05). Additionally, 0.2% GPB was established as the optimum inclusion level. Taken together, these results suggest the potential of utilizing GPB as feed additives to improve growth performance in ruminants by reducing biogenic amines and increasing anti-oxidation.

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Aging in brown fat: Antioxidant defenses and oxidative stress

Cited by 7 publications

References 29 publications

Hibernating Little Brown Myotis (Myotis lucifugus) Show Variable Immunological Responses to White-Nose Syndrome

Hibernating Little Brown Myotis (Myotis lucifugus) Show Variable Immunological Responses to White-Nose Syndrome

Age‐induced oxidative stress impairs adipogenesis and thermogenesis in brown fat

Effect of γ-Aminobutyric Acid (GABA) Producing Bacteria on <italic>In vitro</italic> Rumen Fermentation, Biogenic Amine Production and Anti-oxidation Using Corn Meal as Substrate

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Aging in brown fat: Antioxidant defenses and oxidative stress

Cited by 7 publications

References 29 publications

Hibernating Little Brown Myotis (Myotis lucifugus) Show Variable Immunological Responses to White-Nose Syndrome

Hibernating Little Brown Myotis (Myotis lucifugus) Show Variable Immunological Responses to White-Nose Syndrome

Age‐induced oxidative stress impairs adipogenesis and thermogenesis in brown fat

Effect of γ-Aminobutyric Acid (GABA) Producing Bacteria on &lt;italic&gt;In vitro&lt;/italic&gt; Rumen Fermentation, Biogenic Amine Production and Anti-oxidation Using Corn Meal as Substrate

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Effect of γ-Aminobutyric Acid (GABA) Producing Bacteria on <italic>In vitro</italic> Rumen Fermentation, Biogenic Amine Production and Anti-oxidation Using Corn Meal as Substrate