Aging characteristics of protein precipitates produced by polyelectrolyte precipitation in turbulently agitated reactor

Development of a novel strategy for controlled release of lysozyme from whey protein isolate based active food packaging films

“…PAA-LYS complex formation was dominated by charge neutralization through a pH-dependent protonation and deprotonation binding mechanism (Ghimire et al, 2014), followed by a shearinduced collision based polymer bridging mechanism (Chen et al, 1992;Kim et al, 2002). For all complex formulations, almost 100% LYS loading into complexes was achieved regardless of the PAA molecular weight or PAA/LYS ratio (Table 2).…”

“…PAA/LYS complexes were prepared by the precipitation of lysozyme with polyacrylic acid through charge neutralization and polymer bridging mechanisms (Chen et al, 1992;Ghimire et al, 2014;Kim et al, 2002). Four different complexes were produced through changing the molecular weight of PAA (2 and 450 kDa) and PAA/LYS ratio (PAA/LYS: 0.1 and 0.3 w/w).…”

“…Subsequently these complexes were incorporated into whey protein isolate (WPI) films. Protein polyelectrolyte complex formation is mostly driven by the electrostatic interactions between oppositely charged groups in the protein and the polyelectrolyte (Chen, Walker, & John, 1992;Ghimire, Kasi, & Kumar, 2014;Kamiya & Klibanov, 2003;Kim, Hirasawa, & Kim, 2002). Under certain conditions, the complex is formed spontaneously by mixing solutions of proteins and polyelectrolytes.…”

Design of automatic control system for the precipitation of bromelain from the extract of pineapple wastes

“…Protein precipitation is frequently featured by the spontaneous fractionation and concentration as well as a low additive consumption and protein non-denaturation (KIM; HIRASAWA; KIM, 2002;ARAKAWA;YOSHIKO;TIMASHEFF, 2007;HEDHAMMAR;KARLSTRON;HOBER, 2006). Protein precipitation usually produces insoluble protein by contacting precipitants such as neutral salts, acids, organic solvents, or metallic ions with the desired protein in a stirred tank.…”

Adaptive Control of Bromelain Precipitation in a Fed-Batch Stirred Tank

“…Among the practical methods being applied to the large-scale recovery and purification of proteins from dilute solution, protein precipitation is regarded as a key operational process, which is used during the early stages of the downstream processing. Protein precipitation is frequently featured by the spontaneous fractionation and concentration as well as a low additive consumption and protein nondenaturation (Kim et al, 2002;Chen and Berg, 1993;Clark and Clatz, 1987). Protein precipitation usually produces insoluble protein by contacting precipitants, such as neutral salts, acids, organics solvents, or metallic ions, with the desired protein in a stirred tank.…”