Aging changes in the retina of male albino rat: a histological, ultrastructural and immunohistochemical study

Mohamed, M E I; El-Shaarawy, Ehab Aa; Youakim, Magdy; Shuaib, Doaa; Ahmed, Marwa Mostafa

doi:10.5603/fm.a2018.0075

Cited by 15 publications

(11 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A study comparing young rat retinas to those of elderly and senile rats showed that with aging, the overall thickness of the retina decreases, and in senile retinas, there was a marked decrease in the thickness of the inner nuclear layer, with it only having 2-3 rows of cells. 16,17 Ocular blood flow and its regulation are altered with senescence, and elderly retinas also show, on average, a 20% decrease in macular blood flow. [18][19][20] These agerelated changes in perfusion may further exacerbate the well-described ocular perfusion abnormalities in diabetes, including impaired blood flow regulation.…”

Section: Retinal Changes With Agingmentioning

confidence: 99%

See 1 more Smart Citation

Diabetic Retinopathy in the Aging Population: A Perspective of Pathogenesis and Treatment

Leley¹,

Ciulla²,

Bhatwadekar³

2021

CIA

View full text Add to dashboard Cite

The elderly population in the United States is projected to almost double by the year 2050. In addition, the numbers of diabetics are rising, along with its most common complication, diabetic retinopathy (DR). To effectively treat DR within the elderly population, it is essential first to consider the retinal changes that occur due to aging, such as decreased blood flow, retinal thinning, and microglial changes, and understand that these changes can render the retina more vulnerable to oxidative and ischemic damage. Given these considerations, as well as the pathogenesis of DR, specific pathways could play a heightened role in DR progression in elderly patients, such as the polyol pathway and the vascular endothelial growth factor (VEGF) axis. Current ocular treatments include intravitreal corticosteroids, intravitreal anti-VEGF agents, laser photocoagulation and surgical interventions, in addition to better control of underlying diabetes with an expanding range of systemic treatments. While using therapeutics, it is also essential to consider how pharmacokinetics and pharmacodynamics change with aging; oral drug absorption can decrease, and ocular drug metabolism might affect the dosing and delivery methods. Also, elderly patients may more likely be nonadherent to their medication regimen or appointments than younger patients, and undertreatment with anti-VEGF drugs often leads to suboptimal outcomes. With a rising number of elderly DR patients, understanding how aging affects disease progression, pharmacological metabolism, and adherence are crucial to ensuring that this population receives adequate care.

show abstract

Section: Retinal Changes With Agingmentioning

confidence: 99%

Section: Retinal Changes With Agingmentioning

confidence: 99%

Diabetic Retinopathy in the Aging Population: A Perspective of Pathogenesis and Treatment

Leley¹,

Ciulla²,

Bhatwadekar³

2021

CIA

View full text Add to dashboard Cite

show abstract

“…Degenerative changes caused by aging may affect the sensory experience, including vision, auditory and related cognitive domains [11]. Such changes occur as a part of normal physiological processes and may be irreversible [12]. In this study, the results demonstrated a decrease in metabolic connectivity between brain regions related to sensory experience that provided a basis to explain the pathogenesis of age-associated decline in sensory experience.…”

Section: Discussionmentioning

confidence: 54%

Age-related alterations of brain metabolic network based on [18F]FDG-PET of rats

Xue

Huo

et al. 2022

Aging

View full text Add to dashboard Cite

Using animal models to study the underlying mechanisms of aging will create a critical foundation from which to develop new interventions for aging-related brain disorders. Aging-related reorganization of the brain network has been described for the human brain based on functional, metabolic and structural connectivity. However, alterations in the brain metabolic network of aging rats remain unknown. Here, we submitted young and aged rats to [18F]fluorodeoxyglucose with positron emission tomography (18F-FDG PET) and constructed brain metabolic networks. The topological properties were detected, and the network robustness against random failures and targeted attacks was analyzed for age-group comparison. Compared with young rats, aged rats showed reduced betweenness centrality (BC) in the superior colliculus and a decreased degree (D) in the parietal association cortex. With regard to network robustness, the brain metabolic networks of aged rats were more vulnerable to simulated damage, which showed significantly lower local efficiency and clustering coefficients than those of the young rats against targeted attacks and random failures. The findings support the idea that aged rats have similar aging-related changes in the brain metabolic network to the human brain and can therefore be used as a model for aging studies to provide targets for potential therapies that promote healthy aging.

show abstract

“…Any combination of the above processes will have basic influence on the speed of tissue deterioration and cell death. Since the retina is the metabolically most active organ in the mammalian body [Graymore, 1970;Kumagai, 1999;Nivison-Smith et al, 2015]and there is very limited regenerative capacity in the CNS, it is particularly prone to damage due to aging, which has been demonstrated by several authors [Gao and Holly- field, 1992; Curcio and Drucker, 1993;Panda-Jonas et al, 1995;Kim et al, 1996;Liets et al, 2006;Aggarwal et al, 2007;Parikh et al, 2007;Freund et al, 2011;Samuel et al, 2011;Cunea et al, 2014] in several species including rats [Cano et al, 1986;Papazafiri et al, 1995;Mansour et al, 2008;Nadal-Nicolas et al, 2018a;Mohamed et al, 2019]. The majority of these papers deals with male subjects.…”

Section: Discussionmentioning

confidence: 99%

Age-Related Alterations of Proteins in Albino Wistar Rat Retina

Kovács-Valasek¹,

Pöstyéni²,

Denes³

et al. 2021

Cells Tissues Organs

View full text Add to dashboard Cite

Imbalance of homeostasis causes permanent changes in the body with time. The central nervous system is especially prone to these changes since it possesses limited regenerative capacity. In the retina, neurons are damaged during the aging process, and this eventually leads to deterioration of vision. In our 2-year-long study, we examined genetically closely related rat individuals to disclose the hidden retinal causes of age-associated visual dysfunction. Morphometric analysis showed significant reduction of the retina thickness with aging, particularly that of the inner plexiform layer. To reveal changes between the age groups, we used immunohistochemistry against vesicular glutamate transporter 1 protein for photoreceptor and bipolar cell terminals, Brn3a for ganglion cells, calbindin 28 kDa for horizontal cells, parvalbumin for AII amacrines, protein kinase Cα for rod bipolar cells, tyrosine hydroxylase for dopaminergic cells, glial fibrillary acidic protein for glial cells, and peanut-agglutinin labeling for cones. The most significant decrease was observed in the density of photoreceptor and the ganglion cells in the aging process. By using immunocytochemistry and western blot technique, we observed that calbindin and vesicular glutamate transporter 1 protein staining do not change much with aging; tyrosine hydroxylase, parvalbumin and calretinin showed the highest immunoreactivity during the midlife period. Most interestingly, the level of glial fibrillary acidic protein also changes similarly to the previously named markers. Our results provide further evidence that protein content is modified at least in some cell populations of the rat retina, and the number of retinal cells declined with aging. We conclude that senescence alone may cause structural and functional damage in the retinal tissue.

show abstract

Aging changes in the retina of male albino rat: a histological, ultrastructural and immunohistochemical study

Abstract: Rat retinae clearly undergo age-related morphological changes such changes provide a cellular base for explanation of decreased vision in humans with aging other than reflection errors. Effect of aging was not only qualitative, but also quantitative.

Cited by 15 publications

References 35 publications

Diabetic Retinopathy in the Aging Population: A Perspective of Pathogenesis and Treatment

Diabetic Retinopathy in the Aging Population: A Perspective of Pathogenesis and Treatment

Age-related alterations of brain metabolic network based on [18F]FDG-PET of rats

Age-Related Alterations of Proteins in Albino Wistar Rat Retina

Contact Info

Product

Resources

About