Aging affects the phenotypic characteristics of human periodontal ligament cells and the cellular response to hormonal stimulation in vitro

Lossdörfer, Stefan; Kraus, Dominik; Jäger, Andreas

doi:10.1111/j.1600-0765.2010.01297.x

Cited by 28 publications

(21 citation statements)

References 35 publications

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“…6, 7, 8, 9, 10, 11 However, changes in the microenvironment because of age, hypoxia and inflammation can impair the multiplex differentiation potential of hPDLSCs. 12, 13, 14, 15, 16 Periodontitis is a highly prevalent chronic inflammatory bone disease, and it is the main cause of tooth loss. Growing evidence suggests that inflammation suppresses the regenerative capacity of hPDLSCs.…”

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confidence: 99%

Long noncoding RNA related to periodontitis interacts with miR-182 to upregulate osteogenic differentiation in periodontal mesenchymal stem cells of periodontitis patients

Wang¹,

et al. 2016

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Periodontitis impairs the osteogenic differentiation of human periodontal mesenchymal stem cells (hPDLSCs), but the underlying molecular mechanisms are still poorly understood. Long noncoding RNAs (lncRNAs) have been demonstrated to have significant roles under both physiologic and pathological conditions. In this study, we performed comprehensive lncRNA profiling by lncRNA microarray analysis and identified a novel lncRNA, osteogenesis impairment-related lncRNA of PDLSCs from periodontitis patients (lncRNA-POIR), the expression of which was significantly decreased in PDLSCs from periodontitis patients (pPDLSCs) and was upregulated by osteogenic induction. To study the functions of lncRNA-POIR, we prepared cells with overexpression and knockdown of lncRNA-POIR and found that lncRNA-POIR positively regulated osteogenic differentiation of hPDLSCs and pPDLSCs both in vitro and in vivo. Using quantitative real-time PCRs (qPCRs) and luciferase reporter assays, we demonstrated that lncRNA-POIR may act as a competing endogenous RNA (ceRNA) for miR-182, leading to derepression of its target gene, FoxO1. In this process, lncRNA-POIR and miR-182 suppress each other and form a network to regulate FoxO1. FoxO1 increased bone formation of pPDLSCs by competing with TCF-4 for β-catenin and inhibiting the canonical Wnt pathway. Finally, inflammation increases miR-182 expression through the nuclear factor-κB pathway, and the miR-182 overexpression in the inflammatory microenvironment resulted in an imbalance in the lncRNA-POIR-miR-182 regulatory network. In conclusion, our results provide novel evidence that this lncRNA-miRNA (microRNA) regulatory network has a significant role in osteogenic differentiation of pPDLSCs and that it has potential as a therapeutic target in mesenchymal stem cells during inflammation.

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confidence: 99%

Long noncoding RNA related to periodontitis interacts with miR-182 to upregulate osteogenic differentiation in periodontal mesenchymal stem cells of periodontitis patients

Wang¹,

et al. 2016

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“…These cells are actively involved in collagen secretion and matrix remodeling (Hinz et al, 2012). Aging causes a delay in the regeneration of periodontal tissues (Benatti et al, 2006), probably due to modifications in cell phenotype (Lossdörfer et al, 2010), defects in cell proliferation, and changes in the regulation of inflammatory cytokines (Benatti et al, 2009). In addition, aging may decrease the synthesis of collagen types I and III (Benatti et al, 2008).…”

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confidence: 99%

Defective Wound-healing in Aging Gingival Tissue

Cáceres

Oyarzún

2014

J Dent Res

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Aging may negatively affect gingival wound-healing. However, little is known about the mechanisms underlying this phenomenon. The present study examined the cellular responses associated with gingival wound-healing in aging. Primary cultures of human gingival fibroblasts were obtained from healthy young and aged donors for the analysis of cell proliferation, cell invasion, myofibroblastic differentiation, and collagen gel remodeling. Serum from young and old rats was used to stimulate cell migration. Gingival repair was evaluated in SpragueDawley rats of different ages. Data were analyzed by the Mann-Whitney and Kruskal-Wallis tests, with a p value of .05. Fibroblasts from aged donors showed a significant decrease in cell proliferation, migration, Rac activation, and collagen remodeling when compared with young fibroblasts. Serum from young rats induced higher cell migration when compared with serum from old rats. After TGF-beta1 stimulation, both young and old fibroblasts demonstrated increased levels of alpha-SMA. However, alpha-SMA was incorporated into actin stress fibers in young but not in old fibroblasts. After 7 days of repair, a significant delay in gingival wound-healing was observed in old rats. The present study suggests that cell migration, myofibroblastic differentiation, collagen gel remodeling, and proliferation are decreased in aged fibroblasts. In addition, altered cell migration in wound-healing may be attributable not only to cellular defects but also to changes in serum factors associated with the senescence process.

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“…However, aging affects the phenotypic characteristic and cellular response to hormonal changes. Older patients have been found to have a decreased response to iPTH, suggesting a limited ability for periodontal repair . These factors can be considered in future studies of periodontal regeneration.…”

Section: Periodontal Ligament Stem Cell Differentiationmentioning

confidence: 99%

Periodontal ligament stem cells: an update and perspectives

Pandula

Samaranayake

Jin

et al. 2014

J of Invest & Clin Dent

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Chronic periodontitis is a serious infectious and inflammatory oral disease of humans worldwide. Conventional treatment modalities are effective for controlling periodontal disease. However, the regeneration of damaged periodontal tissues remains a major challenge in clinical practice due to the complex structure of the periodontium. Stem cell-based regenerative approaches combined with the usage of emerging biomaterials are entering a new era in periodontal regeneration. The present review updates the current knowledge of periodontal ligament stem cell-based approaches for periodontal regeneration, and elaborates on the potentials for clinical application.

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Aging affects the phenotypic characteristics of human periodontal ligament cells and the cellular response to hormonal stimulation in vitro

Cited by 28 publications

References 35 publications

Long noncoding RNA related to periodontitis interacts with miR-182 to upregulate osteogenic differentiation in periodontal mesenchymal stem cells of periodontitis patients

Long noncoding RNA related to periodontitis interacts with miR-182 to upregulate osteogenic differentiation in periodontal mesenchymal stem cells of periodontitis patients

Defective Wound-healing in Aging Gingival Tissue

Periodontal ligament stem cells: an update and perspectives

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