Aging affects oxidative state in hippocampus, hypothalamus and adrenal glands of Wistar rats

Siqueira, Ionara Rodrigues; Fochesatto, Cíntia; Torres, Iraci Lucena da Silva; Dalmaz, Carla; Netto, Carlos Alexandre

doi:10.1016/j.lfs.2005.04.044

Cited by 98 publications

(39 citation statements)

References 49 publications

(48 reference statements)

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“…Our experiments confirmed the higher ROS contents of the liver of diabetic rats reported by previous investigations [35] [48] [49]. Higher levels of protein carbonyl groups have also been reported previously for both the liver of rats with streptozotocin-induced diabetes and the plasma of diabetic rats and patients [4] [36] [50].…”

Section: Discussionsupporting

confidence: 92%

“…GSH, in turn, is the substrate of the glutathione peroxidase reaction in the removal of hydrogen peroxide (2GSH + H 2 O 2 → GSSG + 2H 2 O). The latter is precisely the most important reactive oxygen species (ROS) that is detected when the antioxidant activity assays are carried out [49]. It is thus possible that the more pronounced effect of the root extract in diminishing the hepatic ROS levels (see Figure 4 and subsection 3.4) in diabetic rats may be connected in some way to the combined activities of enzymes that generate reducing power in the form of NADPH (e.g., G6PDH) and enzymes that remove H 2 O 2 , as glutathione peroxidase, for example.…”

Section: Discussionmentioning

confidence: 99%

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The in Vitro Antioxidant Capacities of Hydroalcoholic Extracts from Roots and Leaves of Smallanthus sonchifolius (Yacon) Do Not Correlate with Their in Vivo Antioxidant Action in Diabetic Rats

Biazon¹,

Wendt²,

Moreira³

et al. 2016

JBM

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Leaf and root extracts of Smallanthus sonchifolius (yacon), have antihyper-glycemic activity and antioxidant properties. The present study aims to compare the in vivo hepatic antioxidant activity of hydroalcoholic extracts of yacon leaves and roots in rats with streptozotocin-induced diabetes in terms of their in vitro antioxidant capacity. Rats were treated during 14 days with 1060 mg•Kg −1 root extract or 400 mg•Kg −1 leaf extract. The latter was richer in phenolics and possessed a much higher in vitro antioxidant activity. Both extracts prevented hyperglycemia in diabetic rats. The liver of diabetic rats presented increased levels of protein carbonyls and ROS and decreased activities of antioxidant enzymes. Treatment with both root and leaf extracts restored the protein carbonyl levels to normality. The root extract also restored the ROS levels to normality, but the leaf extract was not effective. The root extract was also more effective in restoring the activity of at least two important antioxidant enzymes (glucose 6-phosphate dehydrogenase and glutathione peroxidase). In terms of the antioxidant load (which was 17 times lower in the root extract treatment), the in vivo action of the root extract was more effective than the leaf extract in reducing the hepatic oxidative stress that accompanies diabetes.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

The in Vitro Antioxidant Capacities of Hydroalcoholic Extracts from Roots and Leaves of Smallanthus sonchifolius (Yacon) Do Not Correlate with Their in Vivo Antioxidant Action in Diabetic Rats

Biazon¹,

Wendt²,

Moreira³

et al. 2016

JBM

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“…However, the activities of many pivotal antioxidant enzymes (SOD1, SOD2, catalase, and glutathione peroxidase) decrease in the aged rat [8][9][10][11]. Furthermore, the intracellular glutathione concentration was found to decrease with age in the brain [12,13].…”

Section: Introductionmentioning

confidence: 96%

“…These findings indicate that the antioxidant defense system is deteriorated during aging. Therefore, age-related mitochondria dysfunction and the declined antioxidant defense may cause an imbalance between the production and remove of ROS, thus lead to the elevated oxidative stress during aging, which is observed in the aged rat brain [8].…”

Section: Introductionmentioning

confidence: 99%

“…Protein carbonylation, an irreversible process resulting in loss of function of the oxidatively modified proteins, is a widely used marker for oxidative stress [17]. The elevation of total protein carbonyl level is demonstrated for various brain regions during aging [8,18,19]. Although protein oxidation plays a critical role in agerelated decline, not all proteins are sensitive to oxidative damage, suggesting that many specific targets for protein carbonylations may implicate in aging and age-related degenerative disorders [2].…”

Section: Introductionmentioning

confidence: 99%

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Differential Proteomics Analysis of Specific Carbonylated Proteins in the Temporal Cortex of Aged Rats: The Deterioration of Antioxidant System

Wang

Zhao

et al. 2009

Neurochem Res

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Oxidative stress plays a pivotal role in normal brain aging and various neurodegenerative diseases, including Alzheimer's disease (AD). Irreversible protein carbonylation, a widely used marker for oxidative stress, rises during aging. The temporal cortex is essential for learning and memory and particularly susceptible to oxidative stress during aging and in AD patients. In this study, we used 2-DE, MALDI-TOF/TOF MS, and Western blotting to analyze the differentially carbonylated proteins in the rat temporal cortex between 1-month-old and 24-month-old. We showed that the carbonyl levels of ten protein spots corresponding to six gene products: SOD1, SOD2, peroxiredoxin 1, peptidylprolyl isomerase A, cofilin 1, and adenylate kinase 1, significantly increased in the temporal cortex of aged rats. These proteins are associated with antioxidant defense, the cytoskeleton, and energy metabolism. Several oxidized proteins identified in aged rat brain are known to be involved in neurodegenerative disorders as well. Our findings indicate that these carbonylated proteins may be implicated in the decline of normal brain aging process and provide insights into the mechanisms underlying age-associated dysfunction of temporal cortex.

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Age‐related effects of DHEA on peripheral markers of oxidative stress

Jacob

Janner

Jahn

et al. 2009

Cell Biochemistry & Function

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Ageing is an inevitable biological process characterized by a general decline in various physiological functions. DHEA and DHEAS levels are maximal between the second and third life decades, then start to decline 2% per year, leaving a residual of 10-20% of the peak production by the eighth decade. Erythrocytes are exposed to frequent oxidative stress due to the oxygen radicals continuously generated by haemoglobin auto-oxidation. We investigated DHEA chronic (10 mg/kg, subcutaneously, for 5 weeks) effects over oxidative stress markers in erythrocytes of male Wistar rats of 3, 13 and 18 month-old. In the 13 month-old group, we found increased lipid peroxidation (LPO), superoxide dismutase (SOD), glutathione-S-transferase and catalase activities when compared to the other age groups. DHEA produced a marked increase in LPO of 13 month-old group when compared to its control. DHEA exerted this pro-oxidant effects in all ages studied, especially in age 13 month-old. It seems that at 13 month-old there would be an important depletion of some specific anti-oxidant in order to determine such susceptibility to DHEA effects. Since this approach allows a minimally invasive assessment, it would be useful as a routine method in human clinical studies investigating DHEA effects during the ageing process.

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Aging affects oxidative state in hippocampus, hypothalamus and adrenal glands of Wistar rats

Cited by 98 publications

References 49 publications

The <i>in Vitro</i> Antioxidant Capacities of Hydroalcoholic Extracts from Roots and Leaves of <i>Smallanthus sonchifolius</i> (Yacon) Do Not Correlate with Their <i>in Vivo</i> Antioxidant Action in Diabetic Rats

The <i>in Vitro</i> Antioxidant Capacities of Hydroalcoholic Extracts from Roots and Leaves of <i>Smallanthus sonchifolius</i> (Yacon) Do Not Correlate with Their <i>in Vivo</i> Antioxidant Action in Diabetic Rats

Differential Proteomics Analysis of Specific Carbonylated Proteins in the Temporal Cortex of Aged Rats: The Deterioration of Antioxidant System

Age‐related effects of DHEA on peripheral markers of oxidative stress

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Aging affects oxidative state in hippocampus, hypothalamus and adrenal glands of Wistar rats

Cited by 98 publications

References 49 publications

The &lt;i&gt;in Vitro&lt;/i&gt; Antioxidant Capacities of Hydroalcoholic Extracts from Roots and Leaves of &lt;i&gt;Smallanthus sonchifolius&lt;/i&gt; (Yacon) Do Not Correlate with Their &lt;i&gt;in Vivo&lt;/i&gt; Antioxidant Action in Diabetic Rats

The &lt;i&gt;in Vitro&lt;/i&gt; Antioxidant Capacities of Hydroalcoholic Extracts from Roots and Leaves of &lt;i&gt;Smallanthus sonchifolius&lt;/i&gt; (Yacon) Do Not Correlate with Their &lt;i&gt;in Vivo&lt;/i&gt; Antioxidant Action in Diabetic Rats

Differential Proteomics Analysis of Specific Carbonylated Proteins in the Temporal Cortex of Aged Rats: The Deterioration of Antioxidant System

Age‐related effects of DHEA on peripheral markers of oxidative stress

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The <i>in Vitro</i> Antioxidant Capacities of Hydroalcoholic Extracts from Roots and Leaves of <i>Smallanthus sonchifolius</i> (Yacon) Do Not Correlate with Their <i>in Vivo</i> Antioxidant Action in Diabetic Rats

The <i>in Vitro</i> Antioxidant Capacities of Hydroalcoholic Extracts from Roots and Leaves of <i>Smallanthus sonchifolius</i> (Yacon) Do Not Correlate with Their <i>in Vivo</i> Antioxidant Action in Diabetic Rats