Aggressiveness of Diverse French <i>Aphanomyces euteiches</i> Isolates on Pea Near Isogenic Lines Differing in Resistance Quantitative Trait Loci

Quillévéré‐Hamard, Anne; Roy, Gwenola Le; Lesné, Angélique; May, Christophe Le; Pilet‐Nayel, Marie‐Laure

doi:10.1094/phyto-04-20-0147-r

Cited by 14 publications

(13 citation statements)

References 35 publications

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“…Quillévéré-Hamard et al [ 82 ] evaluated several NILs carrying QTL previously found [ 29 ] to contribute to resistance to ARR under both field and greenhouse conditions and reported a high potential for the use of these QTL in gene pyramiding and MAS breeding. In the current study, 344.1 K SNPs were identified to be polymorphic between the R and S bulks.…”

Section: Discussionmentioning

confidence: 99%

Identification of Novel Genes Associated with Partial Resistance to Aphanomyces Root Rot in Field Pea by BSR-Seq Analysis

Wu¹,

Fredua-Agyeman²,

Strelkov³

et al. 2022

IJMS

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Aphanomyces root rot, caused by Aphanomyces euteiches, causes severe yield loss in field pea (Pisum sativum). The identification of a pea germplasm resistant to this disease is an important breeding objective. Polygenetic resistance has been reported in the field pea cultivar ‘00-2067’. To facilitate marker-assisted selection (MAS), bulked segregant RNA-seq (BSR-seq) analysis was conducted using an F8 RIL population derived from the cross of ‘Carman’ × ‘00-2067’. Root rot development was assessed under controlled conditions in replicated experiments. Resistant (R) and susceptible (S) bulks were constructed based on the root rot severity in a greenhouse study. The BSR-seq analysis of the R bulks generated 44,595,510~51,658,688 reads, of which the aligned sequences were linked to 44,757 genes in a reference genome. In total, 2356 differentially expressed genes were identified, of which 44 were used for gene annotation, including defense-related pathways (jasmonate, ethylene and salicylate) and the GO biological process. A total of 344.1 K SNPs were identified between the R and S bulks, of which 395 variants were located in 31 candidate genes. The identification of novel genes associated with partial resistance to Aphanomyces root rot in field pea by BSR-seq may facilitate efforts to improve management of this important disease.

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Section: Discussionmentioning

confidence: 99%

Identification of Novel Genes Associated with Partial Resistance to Aphanomyces Root Rot in Field Pea by BSR-Seq Analysis

Wu¹,

Fredua-Agyeman²,

Strelkov³

et al. 2022

IJMS

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“…Pea NIL evaluations confirmed the significant effect of Aphanomyces resistance QTL combinations with the major QTL on chromosome 7 in decreasing disease severity and limiting yield reduction in highly infested fields. However, isolates were also identified in French pea fields, which were aggressive on these NILs (Quillévéré‐Hamard et al, 2018; Quillévéré‐Hamard et al, 2020), advocating the pyramiding of multiple resistance alleles for breeding varieties with high and durable levels of resistance (Pilet‐Nayel et al, 2017).…”

Section: Genetic and Genomic Determinants Of Resistance To Major Stresses In Pea And Faba Beanmentioning

confidence: 99%

PeaMUST (Pea MultiStress Tolerance), a multidisciplinary French project uniting researchers, plant breeders, and the food industry

et al. 2021

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The French government has supported as part of its “Investments for the Future” program a 9‐year research project, PeaMUST, devoted to pea and to a lesser extent, faba bean improvement. Focusing on the main causes of yield irregularity that limit pea and faba bean cultivation, an integrated approach, including molecular exploitation of the pea genome sequence, was applied to identify and incorporate favorable alleles and allele combinations in prebreeding material.

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“…LSMean values of root DS scores were used. More details are provided in Quillévéré-Hamard et al (2020).…”

Section: Matrix 20: Aphanomyces Euteiches-pisum Sativummentioning

confidence: 99%

The quasi-universality of nestedness in the structure of quantitative plant-parasite interactions

Moury¹,

Audergon²,

Baudracco-Arnas³

et al. 2021

Preprint

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Understanding the relationships between host range and pathogenicity for parasites, and between the efficiency and scope of immunity for hosts are essential to implement efficient disease control strategies. In the case of plant parasites, most studies have focused on describing qualitative interactions and a variety of genetic and evolutionary models has been proposed in this context. Although plant quantitative resistance benefits from advantages in terms of durability, we presently lack models that account for quantitative interactions between plants and their parasites and the evolution of these interactions. Nestedness and modularity are important features to unravel the overall structure of host-parasite interaction matrices. Here, we analysed these two features on 32 matrices of quantitative pathogenicity trait data gathered from 15 plant-parasite pathosystems consisting of either annual or perennial plants along with fungi or oomycetes, bacteria, nematodes, insects and viruses. The performance of several nestedness and modularity algorithms was evaluated through a simulation approach, which helped interpretation of the results. We observed significant modularity in only six of the 32 matrices, with two or three modules detected. For three of these matrices, modules could be related to resistance quantitative trait loci present in the host. In contrast, we found high and significant nestedness in 30 of the 32 matrices. Nestedness was linked to other properties of plant-parasite interactions. First, pathogenicity trait values were explained in majority by a parasite strain effect and a plant accession effect, with no parasite-plant interaction term. Second, correlations between the efficiency and scope of the resistance of plant genotypes, and between the host range breadth and pathogenicity level of parasite strains were overall positive. This latter result questions the efficiency of strategies based on the deployment of several genetically-differentiated cultivars of a given crop species in the case of quantitative plant immunity.

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Aggressiveness of Diverse French Aphanomyces euteiches Isolates on Pea Near Isogenic Lines Differing in Resistance Quantitative Trait Loci

Cited by 14 publications

References 35 publications

Identification of Novel Genes Associated with Partial Resistance to Aphanomyces Root Rot in Field Pea by BSR-Seq Analysis

Identification of Novel Genes Associated with Partial Resistance to Aphanomyces Root Rot in Field Pea by BSR-Seq Analysis

PeaMUST (Pea MultiStress Tolerance), a multidisciplinary French project uniting researchers, plant breeders, and the food industry

The quasi-universality of nestedness in the structure of quantitative plant-parasite interactions

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