Aggregation state of melittin in lipid vesicle membranes

John, Edgar; Jähnig, Fritz

doi:10.1016/s0006-3495(91)82056-2

Cited by 47 publications

(35 citation statements)

References 25 publications

(56 reference statements)

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“…They conclude that Monte Carlo calculations clearly indicate that FRET is indeed sensitive to domain sizes in the range of 5-50 nm, but that a specific model is required to obtain a value for the domain size [66]. Other interesting membrane FRET studies are mentioned in Refs [70][71][72][73][74][75]. Kleinfeld and Lukacovic successfully applied this idea to locate the tryptophan-109 in cytochrome b5 [68] confirming an earlier conclusion by Fleming et al [69].…”

Section: Fret Theory 1965-2012supporting

confidence: 55%

Förster Theory

Meer

2013

FRET – Förster Resonance Energy Transfer

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Theory without experiment is like the sound of one hand clapping. F€ orster theory is not like that at all. It is a thundering ovation linking theory and experiment by explaining the relationship between spectral overlap, energy transfer, and proximity. This chapter explains F€ orster's contributions to the theory of resonance energy transfer. The readers of this chapter form, no doubt, a highly diverse group of people. Most readers are probably only interested in the bottom line. Others may want to know details. But which details? There are so many. To help students and specialists find what they need, the chapter is presented as a sequence of a large number of sections that are short and focused. Pre-F€ orsterThis section is based on some of the information in the most popular papers by F€ orster [1][2][3][4][5], Chapter 5 of Ref. [6], and Clegg's history of FRET [7]. The emphasis here is on the contributions of F€ orster's predecessors and contemporaries. If you want to know who the scientists were who inspired F€ orster and what the science was that motivated him, you should read his most important papers. His most important, that is, his most cited papers are his papers published in 1946 [1] 1948 [2], and 1949 [4] and reviews published in 1959 [3] and 1965 [5]. F€ orster's papers are not easy to understand. The language is not a problem because four of the five are in English or translated into English. They are difficult because they use a lot of math and complicated spectroscopic concepts. Nevertheless, if you are serious about FRET, you should study them. Start with his 1946 paper [1] and the 1959 review [3]. These papers are much more readable than F€ orster's most cited paper [2], because his 1946 paper presents a very clear verbal description of the essential ideas on which the theory is based and a thorough review of the experimental evidence of the importance of resonance and his 1959 paper is designed to provide a conceptual understanding of the FRET -Förster Resonance Energy Transfer: From Theory to Applications, First Edition. Edited by Igor Medintz and Niko Hildebrandt.

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Section: Fret Theory 1965-2012supporting

confidence: 55%

Förster Theory

Meer

2013

FRET – Förster Resonance Energy Transfer

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“…Among a huge variety of analytical tools used for the quantification of polypeptide oligomerization, one of the leading positions belongs to FRET. 14–17,28 The characteristic nanometer distance scale of energy transfer phenomenon renders this technique particularly sensitive in quantitative analysis of protein clustering. Moreover, FRET can be considered not only as quantitative but also as qualitative method.…”

Section: Discussionmentioning

confidence: 99%

Förster Resonance Energy Transfer Evidence for Lysozyme Oligomerization in Lipid Environment

et al. 2010

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Intermolecular time-resolved and single-molecule Förster resonance energy transfer (FRET) have been applied to detect quantitatively the aggregation of polycationic protein lysozyme (Lz) in the presence of lipid vesicles composed of phosphatidylcholine (PC) and its mixture with 5, 10, 20, or 40 mol % of phosphatidylglycerol (PG) (PG5, PG10, PG20, or PG40, respectively). Upon binding to PC, PG5, or PG10 model membranes, Lz was found to retain its native monomeric conformation, while increasing content of anionic lipid up to 20 or 40 mol % resulted in the formation of Lz aggregates. The structural parameters of protein self-association (the degree of oligomerization, the distance between the monomers in protein assembly, and the fraction of donors present in oligomers) have been derived. The crucial role of the factors such as lateral density of the adsorbed protein and electrostatic and hydrophobic Lz–lipid interactions in controlling the protein self-association behavior has been proposed.

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“…However, a related study in which the N-bromosuccinimide oxidized Trp-19 analogue of melittin has been used indicates the aggregation of melittin bound to fluid phase PC or PG membranes in the presence of NaCl (Talbot et al 1987). In another study, it has been observed that the aggregation of melittin occurs only at special conditions such as high salt concentrations and lipid/melittin ratio below 200:1 (John and Jä hnig 1991). In addition, based on the fluorescence quenching resolved spectra of melittin in lipid bilayers, it has been proposed that melittin bound to DMPC membranes exists in both monomeric as well as aggregated forms in presence of 2 M NaCl (Kaszycki and Wasylewski 1990).…”

Section: Aggregation State Of Melittin In Membranesmentioning

confidence: 98%

Melittin: a Membrane-active Peptide with Diverse Functions

2007

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Melittin is the principal toxic component in the venom of the European honey bee Apis mellifera and is a cationic, hemolytic peptide. It is a small linear peptide composed of 26 amino acid residues in which the amino-terminal region is predominantly hydrophobic whereas the carboxy-terminal region is hydrophilic due to the presence of a stretch of positively charged amino acids. This amphiphilic property of melittin has resulted in melittin being used as a suitable model peptide for monitoring lipid-protein interactions in membranes. In this review, the solution and membrane properties of melittin are highlighted, with an emphasis on melittin-membrane interaction using biophysical approaches. The recent applications of melittin in various cellular processes are discussed.

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Aggregation state of melittin in lipid vesicle membranes

Cited by 47 publications

References 25 publications

Förster Theory

Förster Theory

Förster Resonance Energy Transfer Evidence for Lysozyme Oligomerization in Lipid Environment

Melittin: a Membrane-active Peptide with Diverse Functions

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