Aggregation of ligand-modified liposomes by specific interactions with proteins. I: Biotinylated liposomes and avidin

Lynch, Nancy J.; Kilpatrick, Peter K.; Carbonell, Ruben G.

doi:10.1002/(sici)1097-0290(19960420)50:2<151::aid-bit5>3.0.co;2-l

Cited by 19 publications

(29 citation statements)

References 35 publications

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“…However, the fact that A 0 ≠ 0 (visually in Fig. 6) simply reflects the time for Con A addition and mixing before/when absorbance measurements began, i.e., a result of diffusion-limited cluster aggregation [31,32]. In any event Eq.…”

Section: Fabrication Of the Glucose-sensing Aggregatesmentioning

confidence: 98%

Nanogold-plasmon-resonance-based glucose sensing

Aslan

Lakowicz

Geddes

2004

Analytical Biochemistry

214

127

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Noble metal nanoparticles are well known for their strong interactions with light through the resonant excitations of the collective oscillations of the conduction electrons on the particles, the so-called surface plasmon resonances. The close proximity of two nanoparticles is known to result in a red-shifted resonance wavelength peak, due to near-field coupling. We have subsequently employed this phenomenon and developed a new approach to glucose sensing, which is based on the aggregation and disassociation of 20-nm gold particles and the changes in plasmon absorption induced by the presence of glucose. High-molecular-weight dextran-coated nanoparticles are aggregated with concanavalin A (Con A), which results in a significant shift and broadening of the gold plasmon absorption. The addition of glucose competitively binds to Con A, reducing gold nanoparticle aggregation and therefore the plasmon absorption when monitored at a near-red arbitrary wavelength. We have optimized our plasmonic-type glucose nanosensors with regard to particle stability, pH effects, the dynamic range for glucose sensing, and the observation wavelength to be compatible with clinical glucose requirements and measurements. In addition, by modifying the amount of dextran or Con A used in nanoparticle fabrication, we can to some extent tune the glucose response range, which means that a single sensing platform could potentially be used to monitor microM --> mM glucose levels in many physiological fluids, such as tears, blood, and urine, where the glucose concentrations are significantly different.

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Section: Fabrication Of the Glucose-sensing Aggregatesmentioning

confidence: 98%

Nanogold-plasmon-resonance-based glucose sensing

Aslan

Lakowicz

Geddes

2004

Analytical Biochemistry

214

127

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“…A number of experimental and theoretical studies have focused on studying the kinetic pathways and metastable structures characterizing the avidin-mediated aggregation of biotinated vesicles and related systems. − Many other studies have concentrated on measuring and interpreting the forces and energies involved in avidin−biotin binding and cross-bridging, and their dependence on factors such as membrane fluidity and elasticity. − Among the conclusions of these studies is that the noncovalent avidin−biotin bond is generally much stronger than the “hydrophobic” bond between the hydrocarbon tail of the biotinated lipid and the vesicle membrane. Thus, once the avidin is bound to a biotinated membrane lipid, pulling it off the vesicle would result in detachment of the hydrophobic tail from the membrane rather than in dissociation of the avidin−biotin bond. − …”

Section: Introductionmentioning

confidence: 99%

“…The size and shape of the aggregates formed upon adding avidin to a solution containing biotinated vesicles may strongly depend on kinetic factors associated with the efficient and essentially irreversible binding of the avidin to the biotinated lipids. The rapid, avidin-mediated adhesion of vesicles can result in the formation of long-lived (e.g, fractal) aggregates. − However, the long-time behavior of this system may be quite different since cross-bridged vesicles can still dissociate by breaking the weaker bonds between the biotinated lipids and the vesicle membrane. In other words, the equilibrium state of the system is most likely goverened by the formation and dissociation of these biotinated lipid−vesicle bonds and not the avidin−biotin bonds.…”

Section: Introductionmentioning

confidence: 99%

“…The rapid, avidin-mediated adhesion of vesicles can result in the formation of long-lived (e.g, fractal) aggregates. [4][5][6] However, the long-time behavior of this system may be quite different since cross-bridged vesicles can still dissociate by breaking the weaker bonds between the biotinated lipids and the vesicle membrane. In other words, the equilibrium state of the system is most likely goverened by the formation and dissociation of these biotinated lipid-vesicle bonds and not the avidin-biotin bonds.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Statistical Thermodynamic Model for Cross-Bridge Mediated Condensation of Vesicles

Farbman-Yogev

Bohbot-Raviv

1998

J. Phys. Chem. A

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The phase behavior of a solution containing a mixture of large and small, cross-bridging (“two-sided sticker”) particles is studied using a lattice model analyzed with the aid of mean-field calculations and Monte Carlo simulations. Neither the large nor the small particles interact with each other (except for excluded volume effects). However, the small particles can adsorb onto the surface of one or two large particles, in the latter case providing a cross-bridge, i.e., an adhesive bond, between the large particles. The formulation of the model is motivated by experimental studies involving aqueous solutions of vesicles (the large particles) and biotin−avidin−biotin cross-bridges. This system exhibits a first-order phase transition from a dilute to a condensed phase of vesicles once the average number of stickers per vesicle exceeds a certain threshold value. The statistical thermodynamic description of the system becomes particularly simple upon (Legendre) transformation from the two-component canonical ensemble to a “mixed” ensemble involving a constant chemical potential of the cross-bridge particles. The phase separation behavior of the system is calculated for two sets of molecular parametrs, revealing good qualitative agreement with relevant experiments.

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“…On the other hand, proteinligand specic interactions make it possible to induce high binding affinity and selectivity because the ligands interact only with site-specic amino acid residues inside the target protein with a high binding constant. 24,25 In a previous report, Haupt et al demonstrated the synthesis of MIP microgels as articial enzyme inhibitors using a benzamidine-based functional monomer, where the benzamidine moiety acted as a protein-specic ligand for trypsin. 26 In this paper, we demonstrate a novel synthetic route for producing MIP thin lms that have high affinity and selectivity toward a target protein.…”

Section: Introductionmentioning

confidence: 99%