Aggregation of feline lymphoma cells by hyaluronic acid

Abstract

Addition of media from cultured human sarcoma or glioma cells to cultures of suspended feline lymphoma cells [established line FL 74) induced visible aggregationCells in vivo show complex and specific interactions which inter alia keep them arranged in organs and tissues of fixed composition and topography. Such interactions may be reproduced in vitro, where cells will often organize nonrandomly to form aggregates of similar cells with the exclusion of unlike elements (histiotypic aggregation; see e.g. Oppenheimer and Humphreys, 1971;Moscona, 1971). The nature of the aggregating factors is largely unknown.The present report is concerned with a different kind of aggregating phenomenon, expressed by a single cell type (cat lymphoma) in response to a factor produced by a number of non-related cells. The aggregation factor has been identified as hyaluronic acid (HA), suggesting a hitherto unrecognized biological function for this polysaccharide. These results confirm and extend those of Pessac and Defendi (1972), who observed a similar phenomenon in a murine system. MATERIAL AND METHODS Cell lines and culture conditionsThe derivation of the cell lines employed appears in Table I. The lymphoid cell lines were cultivated in Erlenmeyer flasks as non-stirred semi-suspension cultures, with cell aggregates located on the bottom of the flasks. The sarcoma,

Hyaluronan: fundamental principles and applications in cancer

“…Although demonstrated by the aggregation of certain lymphoma cell lines many years before [39,40], the ability of hyaluronan to adhere to cell surfaces was confirmed by the discovery of the HYA-binding site on SV-3T3 cells [15]. As noted above, this binding site was later defined as CD44, which is responsible for the adhesion of HYA to endothelial cells [22] and human myeloid cell lines [26], and for the adhesion of B-lineage lymphocytes to stroma cells [25].…”

Glycosaminoglycan Synthesis by Wilms' Tumor

“…Hyaluronic acid has recently been implicated in such processes as cell aggregation (23,34) and mesenchymal cell mobility (17,31). Powars et al (24) have speculated that hyaluronic acid may be involved in the growth and control of metastasis of Wilms' tumor.…”

Proteoglycans and cell adhesion

“…Cell surface-associated hyaluronate is involved in both cell-cell and cell-to sulfated glycosaminoglycan substratum adhesion of SV40-3T3 cells (7,71), attachment of chondrosarcoma cells to tissue culture surfaces (72), and the aggregation of a variety of other cell types (7,73,74). Cell surface-associated hyaluronate is involved in both cell-cell and cell-to sulfated glycosaminoglycan substratum adhesion of SV40-3T3 cells (7,71), attachment of chondrosarcoma cells to tissue culture surfaces (72), and the aggregation of a variety of other cell types (7,73,74).…”

“…The presence of cell surface receptors for hyaluronic acid has been inferred by the ability of this polymer to aggregate cells (73,74) and by the binding of radiolabeled hyaluronate t6 fibroblasts (7,(127)(128)(129), which is time-dependent, saturable, reversable and specific. Furthermore, binding interactions of these endogenous receptors are of high affinity (K D = 10 -gM) (7) and are positively cooperative in nature (130).…”