Biophysical Chemistry volume 23, issue 1-2, P115-124 1985 DOI: 10.1016/0301-4622(85)80069-7 View full text
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Tatzuo Ueki, Yuzuru Hiragi, Mikio Kataoka, Yōji Inoko, Yoshiyuki Amemiya, Yoshinobu Izumi, Hiroyuki Tagawa, Yoshio Muroga

Abstract: A rapid mixing system of the stopped-flow type, used with small-angle X-ray scattering equipment using synchrotron radiation, is described. The process of aggregation of bovine serum albumin was traced with a time interval of 50 s, initiated upon cleavage of its disulfide bonds by reduction with dithiothreitol. The results indicate that a 218-fold molar excess of dithiothreitol over the number of moles of disulfide bonds in bovine serum albumin is sufficient to initiate the reaction immediately after mixing, w…

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