Aggregation‐Enhanced Fluorescence in Organically Modified Silica Nanoparticles: A Novel Approach toward High‐Signal‐Output Nanoprobes for Two‐Photon Fluorescence Bioimaging

Kim, Sehoon; Pudavar, Haridas E.; Bonoiu, Adela C.; Prasad, Paras N.

doi:10.1002/adma.200700098

Cited by 196 publications

(142 citation statements)

References 34 publications

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“…This strategy has been successfully used for organic chromophores and europium based luminescent single nanoparticles. [21][22][23][24][25][26][27][28][29][30] Though these nano-materials offer potentially intriguing luminescence properties, detrimental luminescent self-quenching may occur in some doped luminescent nanoparticles prepared by Stöber or ORMOSIL (Organically Modified Silica) emulsion sol-gel techniques. Here we describe the preparation and molecular-level characterization of bright ytterbium-containing luminescent nanoparticles (LSN).…”

Section: In Nt Tr Ro Od Du Uc Ct Ti Io On Nmentioning

confidence: 99%

Near-IR Two Photon Microscopy Imaging of Silica Nanoparticles Functionalized with Isolated Sensitized Yb(III) Centers

et al. 2014

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Section: In Nt Tr Ro Od Du Uc Ct Ti Io On Nmentioning

confidence: 99%

Near-IR Two Photon Microscopy Imaging of Silica Nanoparticles Functionalized with Isolated Sensitized Yb(III) Centers

et al. 2014

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“…Their application is particularly important in biological fields such as bioseparation [7,8], diagnostics [9,10], bioimaging [11,12] and therapy [13][14][15]. Gold nanoparticles (GNPs) with a size ranging from several to hundreds of nanometers show a bright pinkish color due to plasmon resonance; they have been used in stained glasses for several hundred years.…”

Section: Introductionmentioning

confidence: 99%

Engineering of poly(ethylene glycol) chain-tethered surfaces to obtain high-performance bionanoparticles

Nagasaki

2010

Science and Technology of Advanced Materials

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A poly(ethylene glycol)-b-poly [2-(N,N-dimethylamino)ethyl methacrylate] block copolymer possessing a reactive acetal group at the end of the poly(ethylene glycol) (PEG) chain, that is, acetal-PEG-b-PAMA, was synthesized by a proprietary polymerization technique. Gold nanoparticles (GNPs) were prepared using the thus-synthesized acetal-PEG-b-PAMA block copolymer. The PEG-b-PAMA not only acted as a reducing agent of aurate ions but also attached to the nanoparticle surface. The GNPs obtained had controlled sizes and narrow size distributions. They also showed high dispersion stability owing to the presence of PEG tethering chains on the surface. The same strategy should also be applicable to the fabrication of semiconductor quantum dots and inorganic porous nanoparticles. The preparation of nanoparticles in situ, i.e. in the presence of acetal-PEG-b-PAMA, gave the most densely packed polymer layer on the nanoparticle surface; this was not observed when coating preformed nanoparticles. PEG/polyamine block copolymer was more functional on the metal surface than PEG/polyamine graft copolymer, as confirmed by angle-dependent x-ray photoelectron spectroscopy. We successfully solubilized the C 60 fullerene into aqueous media using acetal-PEG-b-PAMA. A C 60 /acetal-PEG-b-PAMA complex with a size below 5 nm was obtained by dialysis. The preparation and characterization of these materials are described in this review.

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“…[31] The x potential of pure SNPs is negatively signed, whose absolute value is increased with an increase in pH of the aqueous medium (Supporting Information, Figure S3). At high pH value or in the basic medium, the acidic hydroxyl groups on the nanoparticle surfaces are converted into basic form, imparting high surface charges and hence good stability to the SNPs.…”

Section: Resultsmentioning

confidence: 98%

Fabrication of Fluorescent Silica Nanoparticles Hybridized with AIE Luminogens and Exploration of Their Applications as Nanobiosensors in Intracellular Imaging

Mahtab

Hong

Liu

et al. 2010

Chemistry A European J

125

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IntroductionFluorescent nanoparticles have been found useful as visualization tools for biological sensing, probing, imaging, monitoring. [1,2] Among nanoparticles, semiconductor quantum dots (QDs) have attracted much attention, particularly in the area of cellular marking or imaging. QDs enjoy such advantages as size-tunable emission color, long luminescence lifetime and resistance to photobleaching. Their surfaces, however, need to be modified in order to improve their hydrophilicity. Recent investigations have revealed that a large portion (> 40 %) of QDs used under biological conditions show dark state. The use of high concentrations of QDs may solve this problem but causes more serious problems such as enhanced cytotoxicity. This is easy to understand, taking into consideration that QDs are usually chalcogenides of heavy metals (e.g., ZnSe, CdS and PdTe), which are well known toxicants or carcinogens. [3][4][5][6] In contrast, silica nanoparticles (SNPs) are hydrophilic and biocompatible. SNPs have found a variety of applications in areas spanning from information technology to biological engineering. Besides being cytophilic, SNPs are transparent but nonfluorescent and hence ideal host materials for the fabrication of fluorescent silica nanoparticles (FSNPs) for imaging purposes. FSNPs can be prepared by incorporating fluorophores into silica networks via physical processes or chemical reactions. The silica matrix acts as a protective shield, reducing the likelihood of penetrations of oxygen and other harmful species that may cause photobleaching of the embedded fluorophores. [7][8][9] For sensitive detection, trace analysis, diagnostic assay, and real-time monitoring, FSNPs should emit intense visible Abstract: Highly emissive inorganicorganic nanoparticles with core-shell structures are fabricated by a one-pot, surfactant-free hybridization process. The surfactant-free sol-gel reactions of tetraphenylethene-(TPE) and silolefunctionalized siloxanes followed by reactions with tetraethoxysilane afford fluorescent silica nanoparticles FSNP-1 and FSNP-2, respectively. The FSNPs are uniformly sized, surface-charged and colloidally stable. [10] A low fluorophore loading in the nanoparticle may be free of aggregation but can offer only weak fluorescence signals. The light emission can further be weakened, rather than enhanced, when more fluorophores are loaded into the nanoparticles, because of the notorious aggregation-caused quenching (ACQ) effect. [11] We have discovered an "abnormal" phenomenon that is exactly opposite to the ACQ effect, namely aggregation-induced emission (AIE). Nonemissive luminogens, such as tetraphenylethene (TPE) and hexaphenylsilole, are induced to emit efficiently by aggregate formation. [12][13][14] The AIE effect dramatically boosts fluorescence quantum yields (F F ) of the luminogens, turning them from faint fluorophores to strong emitters. An extreme example of such AIE luminogen is 4,4'-bis(1,2,2-triphenylvinyl)biphenyl: its emission efficiencies in the solution (F F,S ) and aggre...

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Aggregation‐Enhanced Fluorescence in Organically Modified Silica Nanoparticles: A Novel Approach toward High‐Signal‐Output Nanoprobes for Two‐Photon Fluorescence Bioimaging

Cited by 196 publications

References 34 publications

Near-IR Two Photon Microscopy Imaging of Silica Nanoparticles Functionalized with Isolated Sensitized Yb(III) Centers

Near-IR Two Photon Microscopy Imaging of Silica Nanoparticles Functionalized with Isolated Sensitized Yb(III) Centers

Engineering of poly(ethylene glycol) chain-tethered surfaces to obtain high-performance bionanoparticles

Fabrication of Fluorescent Silica Nanoparticles Hybridized with AIE Luminogens and Exploration of Their Applications as Nanobiosensors in Intracellular Imaging

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