Aggregation and Size Attributes Analysis of Unadsorbed and Adjuvant-adsorbed Antigens using a Multispectral Imaging Flow Cytometer Platform

Choy, Christopher H.; He, Lin; Tulumello, David V.; Gajewska, Beata; Terebiznik, Mauricio R.; Botelho, Roberto J.; Azizi, Ali

doi:10.1016/j.xphs.2021.10.037

Cited by 2 publications

(1 citation statement)

References 36 publications

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“…In this recent publication, the authors showed that different formulation strategies and differences in electrostatic adsorption strength can result in different distribution of antigens and a time-dependent maturation effect to reach homogeneous and monodispersed antigen–AH complexes ( Figure 2 ). Imaging flow cytometry is the latest version of an all-in-one platform for the measurement of Neisseria meningitidis antigens–AP complexes in vaccine formulations, allowing for the detection of protein aggregates [ 88 ].…”

Section: Analytical Characterization Of Aluminum-adsorbed Antigensmentioning

confidence: 99%

Aluminum Adjuvants—‘Back to the Future’

Laera

HogenEsch

O’Hagan³

2023

Pharmaceutics

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Aluminum-based adjuvants will continue to be a key component of currently approved and next generation vaccines, including important combination vaccines. The widespread use of aluminum adjuvants is due to their excellent safety profile, which has been established through the use of hundreds of millions of doses in humans over many years. In addition, they are inexpensive, readily available, and are well known and generally accepted by regulatory agencies. Moreover, they offer a very flexible platform, to which many vaccine components can be adsorbed, enabling the preparation of liquid formulations, which typically have a long shelf life under refrigerated conditions. Nevertheless, despite their extensive use, they are perceived as relatively ‘weak’ vaccine adjuvants. Hence, there have been many attempts to improve their performance, which typically involves co-delivery of immune potentiators, including Toll-like receptor (TLR) agonists. This approach has allowed for the development of improved aluminum adjuvants for inclusion in licensed vaccines against HPV, HBV, and COVID-19, with others likely to follow. This review summarizes the various aluminum salts that are used in vaccines and highlights how they are prepared. We focus on the analytical challenges that remain to allowing the creation of well-characterized formulations, particularly those involving multiple antigens. In addition, we highlight how aluminum is being used to create the next generation of improved adjuvants through the adsorption and delivery of various TLR agonists.

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Section: Analytical Characterization Of Aluminum-adsorbed Antigensmentioning

confidence: 99%

Aluminum Adjuvants—‘Back to the Future’

Laera

HogenEsch

O’Hagan³

2023

Pharmaceutics

View full text Add to dashboard Cite

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Imaging flow cytometry for life sciences

Goncharov¹

2022

jour

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The review presents the basic information about the method of imaging flow cytometry and the main differences from traditional flow cytometry and fluorescence microscopy. The principles of the method, the design of instruments, the process of data collection and analysis are described in detail. The fields of application of the method and its possibilities in the analysis of cells, microorganisms and microparticles are described.

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Aggregation and Size Attributes Analysis of Unadsorbed and Adjuvant-adsorbed Antigens using a Multispectral Imaging Flow Cytometer Platform

Cited by 2 publications

References 36 publications

Aluminum Adjuvants—‘Back to the Future’

Aluminum Adjuvants—‘Back to the Future’

Imaging flow cytometry for life sciences

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