Aggregation and Separation of Hybridoma Cells by Ultrasonic Resonance Fields - Eifect on Viability, Growth Rate and Productivity.

Trampler, Felix; Pui, Phylis W. S.; Sonderhoff, Stefan A.; Kilburn, Douglas G.; Piret, James M.; Benes, E.; Groeschl, Martin; Bürger, Wolfgang

doi:10.1016/b978-0-7506-1877-9.50127-x

Cited by 2 publications

(2 citation statements)

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“…The contamination by discarded nano particles could be prevented by precipitating the nano particles. The precipitation technologies using ultrasound for the recycle of waste water have been suggested [1][2] . However, the mechanism of physical and chemical reactions by ultrasound is not clearly explained and it is known that ultrasonic cavitation in water cause the phenomena [3][4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Precipitation method for nano particle using focused ultrasound

Kim

Hwangbo

et al. 2013

2013 IEEE International Ultrasonics Symposium (IUS)

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An effective ultrasonic precipitation method for the recycle and collection of the nano particles is suggested. We designed a cylindrical piezoelectric transducer to precipitate nano particles effectively. The suspension of Ti02 was circulated through the center of a cylindrical piezoelectric transducer to receive the ultrasonic energy uniformly. The ultrasound from the transducer is focused in the glass tube where the suspension flows. The nano particles started to precipitate 40 minutes after 50 hour-ultrasound irradiation. To compare with other ultrasonic methods such as ultrasonic bath and ultrasonic homogenizer, the precipitated states from each method after ultrasound irradiation were observed. As the result, the suspension irradiated by focused ultrasound showed the best precipitation within the shortest time.

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Section: Introductionmentioning

confidence: 99%

Precipitation method for nano particle using focused ultrasound

Kim

Hwangbo

et al. 2013

2013 IEEE International Ultrasonics Symposium (IUS)

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“…Currently, ultrasonic separation systems have a range of biotechnological applications Bierau et al, 1998;Doblhoff-Dier et al, 1994;Gaida et al, 1995Gaida et al, , 1996Hawkes and Coakley, 1996;Hawkes et al, 1997;Pui et al, 1995;Trampler, 2000;Trampler et al, 1993Trampler et al, , 1994Zhang et al, 1998). One such application is in perfusion cell reactors, to facilitate the automatic change of nutrient medium and harvest of cell products.…”

Section: Introductionmentioning

confidence: 99%

Quantification of a novel h‐shaped ultrasonic resonator for separation of biomaterials under terrestrial gravity and microgravity conditions

Böhm

Briarty

Lowe

et al. 2003

Biotech & Bioengineering

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A novel, h-shaped ultrasonic resonator was used to separate biological particulates. The effectiveness of the resonator was demonstrated using suspensions of the cyanobacterium, Spirulina platensis. The key advantages of this approach were improved acoustic field homogeneity, flow characteristics, and overall separation efficiency (sigma = 1 - ratio of concentration in cleared phase to input), monitored using a turbidity sensor. The novel separation concept was also effective under microgravity conditions; gravitational forces influenced overall efficiency. Separation of Spirulina at cleared flow rates of 14 to 58 L/day, as assessed by remote video recording, was evaluated under both microgravity (

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Aggregation and Separation of Hybridoma Cells by Ultrasonic Resonance Fields - Eifect on Viability, Growth Rate and Productivity.

Cited by 2 publications

References 1 publication

Precipitation method for nano particle using focused ultrasound

Precipitation method for nano particle using focused ultrasound

Quantification of a novel h‐shaped ultrasonic resonator for separation of biomaterials under terrestrial gravity and microgravity conditions

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