Agglutinin Response of Rabbits to Combined Pasteurella tularensis-Brucella abortus Vaccination

Abstract

A schedule was developed for the simultaneous production of a Pasteurella tularensis-Brucella abortus antiserum in rabbits. Three doses of 10 8 viable P. tularensis LVS organisms were given intravenously at weekly intervals. One day prior to the final dose of P. tularensis , the rabbits received 10 9 viable cells of B. abortus strain 19 intravenously. The use of live vaccines, administered in this sequence, resulted in high agglutinin titers within 3 weeks. The maximal agglutinin titer to either organism was observed 1 week after the final injection.