Agglutinability of cattle red cells. 2. Agglutination by isologous and heterologous sera of enzyme‐treated red cells

Abstract

Neuraminidase treatment made cattle red cells (CRC) agglutinable by the agglutinins of the different heterologous but not of the homologous sera. These agglutinins were, however, absorbed by both the nauraminidase-treated and the intact CRC.Proteolytic treatment made CRC aggIutinable also by the norma1 cattle, isoimmune and autologous sera. Agglutination titres of the CRC ranged from 1:2 to 1:256, but the variation between CRC from members of monozygous (MZ) pairs was not greater than zk 2 agglutination score units the range of experimental error.Treatment with trypsin made the A,,A, factors more emergent on the surface of CRC for agglutination by anti-A,,while pronase treatment had a similar effect upon aggIutination of Z-positive cells by anti-Z. r m