Agents Isolated from Vaginal Cultures in the Reproductive Period and Their Antibiotic Sensitivities (Vaginal Culture and Antibiotic Sensitivitie)

Abstract

Introduction: In our study, we aimed to examine the strains isolated from vaginal swab samples sent to our laboratory from various clinics with a pre-diagnosis of vulvovaginitis and antibiotic resistance rates, retrospectively. Methods: ethods: 90 vaginal swab samples of patientswere included into this study. Two samples were taken from each patient and one sample was used for Gram staining. The other sample was inoculated in 5% sheep blood, Eozine Methylene Blue agar and Saboraud dextrose agar. TIdentification of isolated colonies and antibiotic susceptibility studies were carried out with Vitek 2 Compact automated system. Result of the susceptibility testing are reported according to EUCAST criteria. Nugent scoring was used for bacterial vaginosis. Results: Normal vaginal flora elements were found in 66 (73.3 %) of the specimens, and 28 organisms were found in 24 (26.7 %). The distribution of the organisms is as follows: 6 (21.4%) Escherichia coli, 5(17.9%) Streptococcus agalactiae, 5 (17.9%) Gardnerella vaginalis, 3 (10.7%) Candida spp., 3(10.7%) Klebsiella pneumoniae, 3(10.7%) Enterococcus faecalis. Two of the E.coli strains and one of the K.pneumoniae strains are ESBL positive. Of the agents, 21 were isolated from outpatients and 7 from inpatients. All S. agalactiae strains were susceptible to penicillin and ampicillin. E.coli and K.pneumoniae strains, which are the most frequently isolated Gram (-) organisms, are most susceptible to amikacin, carbapenems and tigecycline. Conclusion: In patients with vaginal discharge and itching complaints, determining the organisms with microbiological culture and oriented treatment instead of empirical treatment will be more beneficial for cure.