Ageing-associated aberration in meiosis of oocytes from senescence-accelerated mice

Liu, L.

doi:10.1093/humrep/17.10.2678

Cited by 126 publications

(75 citation statements)

References 68 publications

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“…Of all the endpoints assessed in our study, the increase in incidence of oocytes exhibiting spindle abnormalities, and consequently chromosomal misalignment, in AL-fed females from less than 10% to almost 65% between 3 and 12 mo of age offers the most prominent example of the negative influence of maternal aging on egg quality. Whereas there is some variation in the reported prevalence of these abnormalities in the literature, which may be due to strain-or methodology-related differences, the high rates of chromosomal and spindle abnormalities observed in our study are consistent with previous reports in humans and mice at ages close to the end of their reproductive lifespan (7,8,10,(40)(41)(42)(43).…”

Section: Discussionsupporting

confidence: 91%

Prevention of maternal aging-associated oocyte aneuploidy and meiotic spindle defects in mice by dietary and genetic strategies

Selesniemi

Lee

Muhlhauser

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

174

167

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Increased meiotic spindle abnormalities and aneuploidy in oocytes of women of advanced maternal ages lead to elevated rates of infertility, miscarriage, and trisomic conceptions. Despite the significance of the problem, strategies to sustain oocyte quality with age have remained elusive. Here we report that adult female mice maintained under 40% caloric restriction (CR) did not exhibit aging-related increases in oocyte aneuploidy, chromosomal misalignment on the metaphase plate, meiotic spindle abnormalities, or mitochondrial dysfunction (aggregation, impaired ATP production), all of which occurred in oocytes of age-matched ad libitum-fed controls. The effects of CR on oocyte quality in aging females were reproduced by deletion of the metabolic regulator, peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α). Thus, CR during adulthood or loss of PGC-1α function maintains female germline chromosomal stability and its proper segregation during meiosis, such that ovulated oocytes of aged female mice previously maintained on CR or lacking PGC-1α are comparable to those of young females during prime reproductive life.bioenergetics | fertility | germ cell | oogenesis | ovary

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Section: Discussionsupporting

confidence: 91%

Prevention of maternal aging-associated oocyte aneuploidy and meiotic spindle defects in mice by dietary and genetic strategies

Selesniemi

Lee

Muhlhauser

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

174

167

View full text Add to dashboard Cite

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“…It has been observed that, in the oocytes of elder women, one or more chromosomes were displaced from the metaphase plate during the second meiotic division [6]. It was also found that the rate of abnormal arrangement of chromosomes in the aging mouse oocytes was significantly increased [7,36]. These previous reports are quite consistent with the present mouse study.…”

Section: The Effect Of Aging On Chromosome Architecture Of Mii-arrestsupporting

confidence: 92%

Effects of maternal aging on localizations and expression levels of DNA methyltransferases and chromosome architecture in in-vivo matured mouse oocytes

Tian

Zhang

et al. 2013

Chin. Sci. Bull.

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This paper investigated the age-related changes in the expression patterns of maintenance methyltransferase (DNMT1) and de novo methyltransferases (DNMT3a, 3b, 3L) and the chromosome architecture in in-vivo matured mouse oocytes using two-photon laser-scanning microscope. Our results showed that (1) DNMT1 and DNMT3a, 3b, 3L in the oocytes of pubertal mice were located in the cortical region of oocyte cytoplasm. In aging groups, DNMT1 was also located in the cortical region. However, DNMT3a, 3b, 3L had a relatively wider distribution in the oocyte cytoplasm and appeared near the chromosomes. These differences between pubertal and aging groups suggested that aging might affect DNA methylation; (2) the expression of DNMT1, and DNMT3a, 3b in aging groups increased significantly compared to pubertal groups, while, the expression of DNMT3L decreased. These results might be explained by the compensation mechanism among DNMTs, which might be impervious to aging; (3) aging caused increased errors in the distribution and three-dimensional morphology of chromosomes, including the increased total volume and surface area, the high ratio of height to diameter of a circular cylinder enclosing the chromosomes (H/D). Our work provided morphological information for the studies of age-related decline in oocyte qualities.

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“…Several hypotheses regarding embryo fragmentation have been reported such as programmed cell death (apoptosis) [26], telomere length [27], increased maternal age [28], abnormalities in cytoskeleton and microtubule organization [29,30]. However, the precise underlying mechanism remains controversial and we considered our TLC observation to be a powerful tool to elucidate the dynamic morphology of this phenomenon.…”

Section: Fragmentation (Supplementary Movie 8)mentioning

confidence: 97%

Human Embryonic Behavior Observed with Time-Lapse Cinematography

Mio¹

2014

J Health Med Informat

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To clarify developmental behavior in human embryos, we observed oocytes with in vitro fertilization and intracytoplasmic sperm injection and donated embryos up to 5 days using our original time-lapse cinematography (TLC) system. The TLC analyses demonstrated several novel aspects and events that were not visible by intermittent stillframe microscopy. Here, we present the entire fertilization process in which we founded the fertilization cone, cytoplasmic flare, dynamic change of nuclear precursor body and cytoplasmic halo. We also demonstrated the occurrence of zygotes with a single pronucleus (PN) and uneven 2PN, fragmentation, blastocoel collapse during the blastocyst stage, and the hatching pattern of expanded blastocysts. Observing such morphokineses in human embryos will enhance our understanding of human embryonic behavior up to preimplantation.

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Ageing-associated aberration in meiosis of oocytes from senescence-accelerated mice

Cited by 126 publications

References 68 publications

Prevention of maternal aging-associated oocyte aneuploidy and meiotic spindle defects in mice by dietary and genetic strategies

Prevention of maternal aging-associated oocyte aneuploidy and meiotic spindle defects in mice by dietary and genetic strategies

Effects of maternal aging on localizations and expression levels of DNA methyltransferases and chromosome architecture in in-vivo matured mouse oocytes

Human Embryonic Behavior Observed with Time-Lapse Cinematography

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