Age, sex, and regional differences in scavenger receptor <scp>CD36</scp> in the mouse brain: Potential relevance to cerebral amyloid angiopathy and Alzheimer's disease

Edler, Melissa K.; Johnson, Cooper; Ahmed, Hashim S.; Richardson, Jason

doi:10.1002/cne.25089

Cited by 10 publications

(8 citation statements)

References 76 publications

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“…In AD, the dysregulation of scavenger receptor activity has been implicated in the clearance of Aβ plaques, which are one of the hallmark pathological features of the disease 34 . Previous studies have reported sex differences in scavenger receptor expression in the context of AD 35 . Additionally, we observed differences in GSEA enrichment of neurogenesis pathways in Microglia under AD conditions.…”

Section: Resultsmentioning

confidence: 92%

A Single-cell and Spatial RNA-seq Database for Alzheimer’s Disease (ssREAD)

Wang,

Acosta,

McNutt

et al. 2023

Preprint

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Alzheimer’s Disease (AD) is a neurodegenerative malady predominantly affecting the elderly and exhibits its debilitating effects on a dementia-prone population. Recently, the advent of innovative technologies, such as single-cell and single-nucleus RNA-sequencing (scRNA-seq & snRNA-seq) and spatial transcriptomics (ST), has reformed our investigative approaches toward comprehending AD’s neuropathological intricacies and underpinning regulatory mechanisms, encompassing sub-cellular, cellular, and spatial dimensions. In light of the overwhelming proliferation of single-cell and ST data associated with AD, the imperative for a comprehensive, user-friendly database that addresses the scientific community’s analytical demands has never been more paramount. Introduced initially in 2020, scREAD presented itself as a pioneering repository that systematized publicly available scRNA-seq and snRNA-seq datasets derived from post-mortem human brain tissues and mouse models mirroring AD pathology. Here, we introduce ssREAD, a substantial upgrade over scREAD, enriching the platform with a broader spectrum of datasets, an optimized analytical pipeline, and enhanced usability and visibility. Specifically, ssREAD amalgamates an impressive portfolio of over 189 datasets extracted from 35 distinct AD-related scRNA-seq and snRNA-seq studies, encompassing a staggering 2,572,355 cells. In addition, we have diligently curated and archived 300 ST datasets, originating from 12 human and mouse brain studies, which include two focused on AD and ten control studies. Every dataset within our repository is meticulously annotated, bearing critical identifiers including species, gender, brain region, disease/control status, age, and AD stages. Besides the collection of above datasets in ssREAD, it delivers an exhaustive analysis suite offering cell clustering and annotation, inference of differentially expressed and spatially variable genes, identification of cell-type-specific marker genes and regulons, and spot deconvolution for integrative analysis of ST and scRNA-seq & snRNA-seq data from public domains. All these resources are freely accessible through a user-friendly, consolidated web portal available athttps://bmblx.bmi.osumc.edu/ssread/.

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Section: Resultsmentioning

confidence: 92%

A Single-cell and Spatial RNA-seq Database for Alzheimer’s Disease (ssREAD)

Wang,

Acosta,

McNutt

et al. 2023

Preprint

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“…Indeed, this hypothesis agrees with the lower retinolemia in females and could be due to either a lower hepatic expression of its transporter protein, i.e., RBP4, [37] leading to a lower plasma concentration of VA, or to a difference in expression of Cd36 between males and females. [45] Indeed, it has been shown that this protein plays a role in the mobilization of hepatic VA stores, since Cd36 -/mice accumulated higher hepatic VA stores than wild type mice. [46] The last parameter of hepatic metabolism of VA that was affected by the sex of animals was the relative proportions of each retinyl ester species.…”

Section: Discussionmentioning

confidence: 99%

β‐Carotene Bioavailability and Conversion Efficiency Are Significantly Affected by Sex in Rats

Borel

Troadec

Damiani

et al. 2021

Molecular Nutrition Food Res

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HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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“…To provide comprehensive, visual representations, spatial mappings were created with Adobe Photoshop 2023. These mappings, presented as a six-color gradient scale, encompass eight brain atlas sections [50]. The sections were adapted from the Allen Mouse Brain Atlas (Allen Institute of Brain Science, 2011) and Paxinos and Franklin’s “The Mouse Brain in Stereotaxic Coordinates,” Fifth Edition [50].…”

Section: Methodsmentioning

confidence: 99%

“…Regional immunoreactivity strength score for JNK3. Eight matching sections were selected from each animal and subjected to immunohistochemical staining for JNK3 (DAB) as described previously [50]. Utilizing a BZ-X All-in-One microscope (Keyence, Itasca, IL), a single observer scored the immunoreactivity strength to JNK3.…”

Section: Immunofluorescent (If)mentioning

confidence: 99%

Physiological JNK3 Concentrations Are Higher in Motor-related and Disease-implicated Brain Regions of C57BL6/J Mice

Godieva,

Sammoura,

Verrier Paz

et al. 2024

Preprint

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The c-Jun N-terminal kinase 3 (JNK3) is a stress-responsive protein kinase primarily expressed in the central nervous system (CNS). JNK3 exhibits nuanced neurological activities, such as roles in behavior, circadian rhythms, and neurotransmission, but JNK3 is also implicated in cell death and neurodegeneration. Despite the critical role of JNK3 in neurophysiology and pathology, its localization in the brain is not fully understood due to a paucity of tools to distinguish JNK3 from other isoforms. While previous functional and histological studies suggest locales for JNK3 in the CNS, a comprehensive and higher resolution of JNK3 distribution and abundance remained elusive. Here, we sought to define the anatomical and cellular distribution of JNK3 in adult mouse brains. Data reveal the highest levels of JNK3 and pJNK3 were found in the cortex and the hippocampus. JNK3 possessed neuron-type selectivity as JNK3 was present in GABAergic, cholinergic, and dopaminergic neurons, but was not detectable in VGLUT-1-positive glutamatergic neurons and astrocytes in vivo. Intriguingly, higher JNK3 signals were found in motor neurons and relevant nuclei in the cortex, basal ganglia, brainstem, and spinal cord. While JNK3 was primarily observed in the cytosol of neurons in the cortex and the hippocampus, JNK3 appeared commonly within the nucleus in the brainstem. These distinctions suggest the potential for significant differences between JNK3 actions in distinct brain regions and cell types. Our results provide a significant improvement over previous reports of JNK3 spatial organization in the adult CNS and support continued investigation of JNK3 actions in neurophysiology and pathophysiology.

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Age, sex, and regional differences in scavenger receptor CD36 in the mouse brain: Potential relevance to cerebral amyloid angiopathy and Alzheimer's disease

Cited by 10 publications

References 76 publications

A Single-cell and Spatial RNA-seq Database for Alzheimer’s Disease (ssREAD)

A Single-cell and Spatial RNA-seq Database for Alzheimer’s Disease (ssREAD)

β‐Carotene Bioavailability and Conversion Efficiency Are Significantly Affected by Sex in Rats

Physiological JNK3 Concentrations Are Higher in Motor-related and Disease-implicated Brain Regions of C57BL6/J Mice

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