Age-Related Telomere Shortening Occurs in Lens Epithelium from Old Rats and is Slowed by Caloric Restriction

Pendergrass, William R.; Penn, Philip; Li, J; Wolf, Norman S.

doi:10.1006/exer.2001.1033

Cited by 44 publications

(34 citation statements)

References 49 publications

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“…Telomere shortening has also been reported in vivo in lens epithelium in old rats (Pendergrass et al 2001), microglia in cerebellum and cortex (Flanary and Streit 2003), and in heart (Kajstura et al 2000;Hastings et al 2004), kidney, lung, pancreas and liver of rats with increasing age (Jennings et al 1999;Cherif et al 2003). However, in separate studies, no telomere shortening was found with increasing cellular senescence with age in rat kidneys (Melk et al 2003;Hastings et al 2004), or in brain (Cherif et al 2003).…”

Section: Other Rodentsmentioning

confidence: 85%

Telomeres and Telomerase Biology in Vertebrates: Progress Towards a Non-Human Model for Replicative Senescence and Ageing

Davis

Kipling

2005

Biogerontology

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Studies on telomere and telomerase biology are fundamental to the understanding of human ageing and age-related diseases such as cancer. However, human studies of whole body ageing are hampered by the lack of suitable fully reflective animal model systems, the wild-type mouse model being unsuitable due to differences in telomere biology. Here we summarise recent data on the biology of telomeres, telomerase, and the tumour suppressor protein p53 in various animals, and examine their possible roles in replicative senescence, ageing, and tumourigenesis. The advantages and disadvantages of various animals as model systems for whole body ageing in humans are discussed.

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Section: Other Rodentsmentioning

confidence: 85%

Telomeres and Telomerase Biology in Vertebrates: Progress Towards a Non-Human Model for Replicative Senescence and Ageing

Davis

Kipling

2005

Biogerontology

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“…The characteristics of aging of osteogenic PDLF and the depletion of non-aged PDLF in periodontal ligament may be one of the causes of defects in the integrity of individual periodontal homeostasis, without correlation to chronological age. The implications of telomere shortening of PDLF in aging in somatic tissue should be considered under conditions normalized for genetic and oral environmental factors since the telomere is a dynamic nucleoprotein complex that can switch stochastically between capped and uncapped states (Blackburn 2000;Pendergrass et al 2001). Further studies of telomeric attrition of osteogenic PDLF with genetic and environmental factors are required.…”

Section: Reduction Of Alpase Activity In Aged Osteogenic Pdlfmentioning

confidence: 98%

Reduction of alkaline phosphatase activity in aged human osteogenic periodontal ligament fibroblasts exhibiting short telomeres

Sawa

Yamaoka

Kuroshima

et al. 2004

Cell and Tissue Research

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The osteogenic cell type of human periodontal ligament fibroblasts (PDLF) undergoes senescence at finite population doubling numbers unrelated to donor ages. This study investigated telomere lengths of osteogenic PDLF from differently aged donors and alterations of the osteoblast-like properties in the aged PDLF with short telomeres. Telomere lengths of osteogenic PDLF were biased towards long or short among all 15- to 51-year-old individuals, and did not show a normal distribution by Pearson's test or a correlation to donor age by simple regression analysis. In osteogenic PDLF, senescence-associated beta-galactosidase was expressed in 78.5% of cells in the clones with short telomeres (mean 3.02 kbp), and in 9.4% of cells in the clones with long telomeres (mean 13.06 kbp). These results suggest that human periodontium comprises aged osteogenic PDLF without correlation to age. Osteogenic PDLF with long telomeres strongly expressed alkaline phosphatase (ALPase) activity whereas cells with short telomeres expressed ALPase activity to a weaker extent. Total activity of ALPase in the clones of osteogenic PDLF with long telomeres was significantly higher than that in the clones with short telomeres. The produced amounts of both osteopontin and osteocalcin in the clones of osteogenic PDLF with long telomeres were slightly but statistically significantly smaller than those in the clones with short telomeres. These findings suggest that aged osteogenic PDLF reduce the expression of ALPase activity but that there is not a critical alteration in bone-associated protein production. Aged osteogenic PDLF may impair the ability to induce ALPase-dependent calcification.

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“…The mice were obtained from the National Institute of Aging colony (NIA, Bethesda, MD), and maintained under specific pathogen free conditions until sacrifice. The mice were sacrificed and lenses removed as described by Pendergrass et al (19). The lenses were washed in Dulbecco's minimal essential medium (DMEM) medium containing 25 mM HEPES and 10% FBS to avoid changes in pH during manipulation, cell isolation and staining.…”

Section: Preparation Of Viable Mouse Lenses For Analysis With Lscmmentioning

confidence: 99%

Efficacy of MitoTracker Green™ and CMXrosamine to measure changes in mitochondrial membrane potentials in living cells and tissues

2004

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Background: Chloromethyl-X-rosamine (CMXRos) and MitoTracker Green (MTG) have proved to be useful dyes with which to measure mitochondrial function. CMXRos is a lipophilic cationic fluorescent dye that is concentrated inside mitochondria by their negative mitochondrial membrane potential (MMP). MTG fluorescence has been used as a measure of mitochondrial mass independent of MMP. The fluorescence ratio of the two dyes is a relative measure of the MMP independent of mitochondrial mass. Because MTG was recently reported to be sensitive to MMP, we have reevaluated the effects of loss of MMP on MTG and CMXRos fluorescence, using both flow cytometry and laser scanning confocal microscopy (LSCM). Methods: Using flow cytometry, the relative fluorescence of CMXRos, R123, and MTG was determined in human lymphoblastoid cell lines (LCLs) with or without carbonyl cyanide p-trifluoromethoxylphenyl-hydrazone (FCCP), used to collapse the MMP. LSCM analysis was also used to evaluate the effect of FCCP on MTG and CMXRos fluorescence of mouse cells and viable lenses in culture. The cytotoxicity of the dyes was determined using flow anal-

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Age-Related Telomere Shortening Occurs in Lens Epithelium from Old Rats and is Slowed by Caloric Restriction

Cited by 44 publications

References 49 publications

Telomeres and Telomerase Biology in Vertebrates: Progress Towards a Non-Human Model for Replicative Senescence and Ageing

Telomeres and Telomerase Biology in Vertebrates: Progress Towards a Non-Human Model for Replicative Senescence and Ageing

Reduction of alkaline phosphatase activity in aged human osteogenic periodontal ligament fibroblasts exhibiting short telomeres

Efficacy of MitoTracker Green™ and CMXrosamine to measure changes in mitochondrial membrane potentials in living cells and tissues

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