Age‐related sensitization profiles for hazelnut (<i>Corylus avellana</i>) in a birch‐endemic region

Knop, K.J. De; Verweij, Marjoke; Grimmelikhuijsen, M.; Philipse, E.; Hagendorens, Margo M.; Bridts, Chris H.; Clerck, Luc S. De; Stevens, W. J.; Ebo, Didier G.

doi:10.1111/j.1399-3038.2011.01112.x

Cited by 81 publications

(82 citation statements)

References 29 publications

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“…Second, 75 patients with a definite history of immediate allergic symptoms due to consumption of raw or processed hazelnuts were included. These were stratified into 6 groups according to the extent of the clinical reaction (OAS or generalized reaction) and age (0-6, 7-18 and >18 years) as detailed elsewhere [4,8,9]. Finally, 15 age-matched hazelnut-tolerant individuals with a positive sIgE to hazelnut and 15 nonallergic and nonsensitized age-matched individuals served as a control group.…”

Section: Methodsmentioning

confidence: 99%

“…Hazelnut allergy has been shown to exhibit distinct geographic and age-related phenotypes, depending on incompletely disentangled sensitization profiles of the individual patient [3,4,5,6,7]. …”

Section: Introductionmentioning

confidence: 99%

“…In contrast, preschool and school-aged children frequently display more generalized reactions that is often related to sensitization to hazelnut storage proteins such as the legumin-like Cor a 9 [4] and the vicilin Cor a 11 [8]. Sensitization to both of these hazelnut components can occur at very young age (<1 year) [8,9] and does not result from cross-reactivity with pollen allergens.…”

Section: Introductionmentioning

confidence: 99%

“…Sensitization to both of these hazelnut components can occur at very young age (<1 year) [8,9] and does not result from cross-reactivity with pollen allergens. From 2 series [4,8], it emerged that no sensitization pattern could be established in about one third of our children with a pollen-unrelated hazelnut allergy. It is likely that the exact reason(s) for this observation relate(s) to the fact that the panel of purified and recombinant proteins available at that time did not cover the entire hazelnut IgE response.…”

Section: Introductionmentioning

confidence: 99%

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Cor a 14: Missing Link in the Molecular Diagnosis of Hazelnut Allergy?

Faber

Graag

Heijden

et al. 2014

Int Arch Allergy Immunol

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Background: Hazelnut allergy shows distinct clinical patterns that can be predicted through component-resolved diagnosis. However, identification of sensitization profiles remains incomplete. Methods: Sera of 75 patients allergic to hazelnuts, 14 infants with atopic dermatitis (AD) sensitized to hazelnuts, 15 hazelnut-tolerant individuals with specific IgE (sIgE) to hazelnuts and 15 healthy control individuals were tested for sIgE reactivity to rCor a 1.04, rCor a 8, nCor a 9, nCor a 11, rCor a 14, rBet v 1, rBet v 2 and cross-reactive carbohydrate determinants (CCDs). Results: Sensitization to Cor a 14 was observed in 18 out of 20 preschool children, 8 out of 10 school-aged children and 2 out of 7 adults with generalized reactions and in 3 out of 14 infants with AD. Only 2 out of 38 patients with an oral allergy syndrome (OAS) were sensitized to Cor a 14. No sensitization to Cor a 14 was observed in the group of hazelnut-tolerant and healthy control individuals. Sensitization to Cor a 1.04 was seen in 36 out of 38 OAS patients and in 14 out of 37 patients with generalized reactions. However, only 3 patients with generalized reactions were monosensitized to Cor a 1.04. Sensitization to Cor a 9 was observed in 26 out of 37 patients with generalized reactions and in 4 out of 14 infants with AD. Sensitization to Cor a 11, Cor a 8, rBet v 2 and CCDs was rare. Conclusions: Sensitization to Cor a 14 can have early onset and shows age-related variations. Together with Cor a 9, Cor a 14 enables us to correctly identify almost 90% of children with generalized reactions to hazelnut.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cor a 14: Missing Link in the Molecular Diagnosis of Hazelnut Allergy?

Faber

Graag

Heijden

et al. 2014

Int Arch Allergy Immunol

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“…Taking into consideration that the difference in sensitization patterns may vary according to geographical and socio-cultural conditions, (10)(11)(12) it is appropriate to put into perspective some geographical aspects of the study area before discussing the results of the study. The Aburrá Valley area is a basin located between two mountain ranges at an altitude of 1,479 meters above sea level.…”

Section: Discussionmentioning

confidence: 99%

Evitación de mascotas en alergias ¿Es la evitación posible de aplicar?

2015

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Cor a 14, the allergenic 2S albumin from hazelnut, is highly thermostable and resistant to gastrointestinal digestion

Pfeifer

Bublin

Dubiela

et al. 2015

Molecular Nutrition Food Res

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ScopeAllergens from nuts frequently induce severe allergic reactions in sensitive individuals. The aim of this study was to elucidate the physicochemical characteristics of natural Cor a 14, the 2S albumin from hazelnut.Methods and resultsCor a 14 was purified from raw hazelnuts using a combination of precipitation and chromatographic techniques. The protein was analyzed using gel electrophoresis, MS, and far‐UV circular dichroism (CD) analyses. The immunoglobulin E (IgE) binding of native, heat‐treated, and in vitro digested Cor a 14 was studied. We identified two different Cor a 14 isoforms and showed microclipping at the C‐terminus. CD spectra at room temperature showed the typical characteristics of 2S albumins, and temperatures of more than 80°C were required to start unfolding of Cor a 14 demonstrating its high stability to heat treatment. In vitro digestion experiments revealed that Cor a 14 is resistant to proteolytic degradation. Native and heat‐treated protein was recognized by sera from hazelnut allergic patients. However, denaturation of the allergen led to significantly reduced IgE binding.ConclusionWe identified two different isoforms of Cor a 14 displaying high stability under heating and gastric and duodenal conditions. Data from IgE‐binding experiments revealed the existence of both, linear and conformational epitopes.

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Age‐related sensitization profiles for hazelnut (Corylus avellana) in a birch‐endemic region

Cited by 81 publications

References 29 publications

Cor a 14: Missing Link in the Molecular Diagnosis of Hazelnut Allergy?

Cor a 14: Missing Link in the Molecular Diagnosis of Hazelnut Allergy?

Evitación de mascotas en alergias ¿Es la evitación posible de aplicar?

Cor a 14, the allergenic 2S albumin from hazelnut, is highly thermostable and resistant to gastrointestinal digestion

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