Age-related lectin histochemical changes in the porcine small intestine.

Lectin Histochemistry of Peyer's Patches in the Porcine Ileum.

“…However, staining patterns were found to differ among lectin groups as well as within the same group. We have no explanation for this discrepancy, but other studies have shown that lectins of the same nominal specificity recognize different features of these complex carbohydrates [3,6,13]. Cell surface markers are of two major types; surface antigens detectable by antibodies against CD proteins, and surface saccharides detectable by lectins [12].…”

“…Therefore, lectins are useful tools in studying glycoprotein and glycolipid structures of the cell surface, specifically in relation to lymphoid cells [3,15,16]. The objective of the present study was to determine cell markers which would be applicable to the characterization of porcine ileal Peyer's patches using lectin probes.…”

“…Tissue sections were stained for lectin binding sites using a modification of the avidin-biotin-peroxidase complex (ABC) method as previously described [3,10,13]. All procedures were performed at room temperature.…”

“…Cell surface markers are of two major types; surface antigens detectable by antibodies against CD proteins, and surface saccharides detectable by lectins [12]. Lectin histochemistry also offers major advantages over using formalin-fixed, paraffinembedded tissues [2,3,13]. Therefore, lectins are potentially useful markers for differentiating lymphoid tissues.…”

Lectin Histochemistry for Glycoconjugates in the Small Intestines of Piglets Naturally Infected with Isospora suis.

“…Tissue sections were stained for lectin binding sites using a modification of the avidin-biotin-peroxidase complex (ABC) method as previously described [2,4,10]. All procedures were performed at room temperature.…”