Age-related Increases in Plasma Phosphatidylcholine Hydroperoxide Concentrations in Control Subjects and Patients with Hyperlipidemia

Abstract

Background: The basal lipid peroxide concentration in the plasma of patients with hyperlipidemia may be related to atherosclerosis. Quantitative determination of lipid peroxides in the plasma is an important step in the overall evaluation of the biochemical processes leading to oxidative injury. Unfortunately, the currently available methods for lipid peroxidation lack specificity and sensitivity. Methods: Hyperlipidemic patients (44 males and 50 females), ages 12–82 years (mean ± SE, 53 ± 2.3 years for males, 58 ± 2.0 years for females, and 56 ± 14 years for total cases), and normolipidemic volunteers (controls, 32 males and 15 females), ages 13–90 years (49 ± 4 years for males, 65 ± 4 years for females, and 55 ± 24 years for total cases), were recruited in the present study. Plasma phosphatidylcholine hydroperoxide (PCOOH) was determined by chemiluminescence-HPLC (CL-HPLC). Results: Plasma PCOOH concentrations increased with age in both controls and hyperlipidemic patients. However, the mean plasma PCOOH concentration in patients with hyperlipidemia (331 ± 19 nmol/L; n = 94) was significantly (P <0.001) higher than in the controls (160 ± 65 nmol/L; n = 47). Plasma PCOOH concentrations were similar in three hyperlipidemic phenotypes: hypercholesterolemia (IIa), hypertriglyceridemia (IV), and combined hyperlipidemia (IIb). The mean plasma PCOOH in patients with treatment-induced normalized plasma lipids was 202 ± 17 nmol/L. There was no significant correlation between plasma PCOOH concentration and total cholesterol, triglycerides, or phospholipids in hyperlipidemic patients. For all subjects, there was a significantly positive correlation between plasma PCOOH and each lipid (total cholesterol, P = 0.0002; triglycerides, P = 0.0137; and phospholipids, P <0.0001). Analysis of fatty acids composition of plasma phosphatidylcholine showed significantly low concentrations of n-6 fatty acids moieties (linoleic acid and arachidonic acid) in patients compared with controls. Conclusions: Our results suggest that an increase in plasma PCOOH in patients with hyperlipidemia may be related to the development and progression of atherosclerosis, particularly in the elderly. Measurement of plasma PCOOH is useful for in vivo evaluation of oxidative stress.

Tandem Mass Spectrometry Analysis of Amadori‐Glycated Phosphatidylethanolamine in Human Plasma

“…Radical-mediated lipid peroxidation plays a central role in the pathophysiology of human disease. 1,2 In a previous paper, we confirmed that synthetic Amadoriglycated phosphatidylethanolamine (Amadori-PE) induces lipid peroxidation via reactive oxygen species generation in the presence of transition metals. 3 Therefore, we hypothesized that glycation of membrane aminophospholipids should be one of the critical risk factors on membrane lipid peroxidation in vivo and thus have put our efforts into the analysis of Amadori-PE in various samples.…”

Phase 1 study of multiple biomarkers for metabolism and oxidative stress after one‐week intake of broccoli sprouts

“…Non-smokers had significant decreases in 8-isoprostane but there were great differences between individuals. PCOOH is associated with core lipid oxidation and reflects in vivo oxidative damage to organs [11]. CoQ 10 H 2 is the first antioxidant when lipoproteins are exposed to free radicals [12].…”

Aging in vertebrates, and the effect of caloric restriction: a mitochondrial free radical production–DNA damage mechanism?

“…In studies of lipid peroxidation during aging, most investigations have measured poorly specific markers. However, recent studies using highly specific high performance liquid chromatography techniques have shown that membrane phospholipid hydroperoxides increase with donor age in red blood cells and plasma of healthy human subjects (Miyazawa et al, 1996;Kinoshita et al, 2000) and in rodent tissues (Tahara, Matsuo & Kaneko, 2001).…”