Age-related effects on induction of DNA strand breaks by intermittent exposure to electromagnetic fields

Ivancsits, Sabine; Diem, E; Jahn, O; Rüdiger, H. W.

doi:10.1016/s0047-6374(03)00125-8

Cited by 44 publications

(18 citation statements)

References 28 publications

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“…Another study, conducted on mouse m5S cells, detected a significant, dosedependent increase of chromatid-type chromosomal aberrations at 5, 50 and 400 mT (Yaguchi et al 2000). Ivancsits et al (2002Ivancsits et al ( , 2003aIvancsits et al ( , 2003b reported an increase in DNA single-and double-strand breaks in human fibroblasts intermittently (5 0 on/10 0 off) exposed to a 50-Hz magnetic field at 1 mT. Pasquini et al (2003) observed an increased frequency of micronuclei in Jurkat cells exposed for 24 h to 5 mT (50 Hz) magnetic fields.…”

Section: Introductionmentioning

confidence: 99%

Clastogenicity and aneuploidy in newborn and adult mice exposed to 50 Hz magnetic fields

Udroiu

Cristaldi

Ieradi

et al. 2006

International Journal of Radiation Biology

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Purpose: To detect possible clastogenic and aneugenic properties of a 50 Hz, 650 mT magnetic field. Materials and methods: The micronucleus test with CREST (Calcinosis, Raynaud's phenomenon, Esophageal dismotility, Sclerodactility, Telangectasia) antibody staining was performed on liver and peripheral blood sampled from newborn mice exposed to an ELF (Extremely Low Frequency) magnetic field during the whole intra-uterine life (21 days), and on bone marrow and peripheral blood sampled from adult mice exposed to the same magnetic field for the same period. Results: Data obtained in newborn mice show a significant increase in micronuclei frequencies. In absolute terms, most of the induced micronuclei were CREST-negative (i.e., formed by a chromosome fragment). However, in relative terms, ELF exposure caused a two-fold increase in CREST-negative micronuclei and a four-fold increase in CREST-positive micronuclei (i.e., formed by a whole chromosome). No significant effect was recorded on exposed adults. Conclusions: These findings suggest the need for investigation of aneugenic properties of ELF magnetic fields in order to establish a possible relationship to carcinogenesis.

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Section: Introductionmentioning

confidence: 99%

Clastogenicity and aneuploidy in newborn and adult mice exposed to 50 Hz magnetic fields

Udroiu

Cristaldi

Ieradi

et al. 2006

International Journal of Radiation Biology

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“…Comparable results were observed by Luceri et al (2005) who failed to observe an increase in DNA damage in human blood lymphocytes exposed to 50 Hz magnetic fields at 1, 10 or 100 µT for 18 hours and more recently by Focke et al (2010), who designed a study in an attempt to validate similar previously conducted studies (Ivancsits et al, 2002(Ivancsits et al, , 2003a(Ivancsits et al, , 2003b). Focke and co-authors (2010) were able to replicate an ELF-MF exposure dependent increase in comet tail DNA using three cell models of primary human fibroblasts (ES-1, HR-1d and MRC-5) intermittently exposed to 50 Hz ELF-MF at a flux density of 1 mT.…”

Section: Experimental Studiesmentioning

confidence: 54%

“…Finally following this series of experiments the authors were able to define the optimum conditions at which the maximum effect on SSB-and DSB was evoked: 100 µT, intermittent (5 min on/10 min off), for 15 hours with human fibroblast cells from donors over 40 years of age, produced a ~2-fold increase in DSB and a 4-fold increase in DSB plus SSB (Ivancsits et al, 2003a(Ivancsits et al, , 2003b.…”

Section: Experimental Studiesmentioning

confidence: 99%

“…Therefore, it is probable that the use of intermittent fields similar to those employed in previous studies (Ivancsits et al, 2002(Ivancsits et al, , 2003a(Ivancsits et al, , 2003bWinker et al, 2005;Burdak-Rothkamm et al, 2009;Saha et al, 2014), would not only have allowed for a more realistic mimicking of global exposure but would have allowed data between studies to be more accurately compared.…”

Section: Study Limitationsmentioning

confidence: 99%

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The effects of extremely low frequency magnetic fields on mutation induction in mice

Wilson

Haines

Sienkiewicz

et al. 2015

Mutation Research/Fundamental and Molecular Mechanisms of Mutag

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“…It was presumed that these effects were caused by the magnetic rather than the electric field [22], as indicated by Brocklehurst at al., who emphasized perturbation of free-radical recombination by magnetic fields [23]. Ivancsits et al showed that different results from studies on continuous and intermittent exposure may be due to a slow induction of DNA-repair processes when cells are exposed intermittently as evidenced by the appearance of single strand breaks (SSB) and double-strand DNA breaks (DSB) in the DNA of human fibroblasts [24]. But the question is whether SSB represented lesions were produced by EMF, or they were intermediates in the repair of other lesions such as oxidized bases [3]?…”

Section: Discussionmentioning

confidence: 99%

Effect of ELF-EMF on antioxidant status and micronuclei in K562 cells and normal lymphocytes

et al. 2014

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The effect of ELF-EMF on DNA through changes in antioxidative enzyme activities has not been sufficiently explored yet. The aim of this study was to determine ELF-EMF effect on antioxidative enzymes in cancer cell line and genotoxic potential on normal human lymphocytes. K562 cells were exposed to 50 Hz ELF-EMF (40 μT, 100 μT; 3 h, 24 h) and spectrophotometric determination of lipid peroxidation and antioxidative enzyme activities was conducted. Genotoxicity of ELF-EMF (50 Hz, 100 μT) was investigated by cytokinesis-block micronucleus assay in a normal human lymphocytes (exposure 24 h and 48 h). Results demonstrated that ELF-EMF did not alter the process of lipid peroxidation and superoxide dismutase activity. Catalase activity was increased only after application of 100 μT EMF for 24 h. Glutathione-S-transferase and -reductase activities were increased. Treatment with 100 μT ELF-EMF (24 h, 48 h) significantly reduced micronuclei incidence, while cell proliferation was significantly increased. Results indicate that 50 Hz ELF-EMF (40 μT, 100 μT) are week stressors which alone cannot generate enough ROS to induce process of lipid peroxidation in cancer cell line but strong enough to induce response of antioxidative system. Furthermore, 100 μT ELF-EMF in human lymphocytes did not exhibit genotoxic potential during 24 h and 48 h treatment, but stimulated cell proliferation.

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Age-related effects on induction of DNA strand breaks by intermittent exposure to electromagnetic fields

Cited by 44 publications

References 28 publications

Clastogenicity and aneuploidy in newborn and adult mice exposed to 50 Hz magnetic fields

Clastogenicity and aneuploidy in newborn and adult mice exposed to 50 Hz magnetic fields

The effects of extremely low frequency magnetic fields on mutation induction in mice

Effect of ELF-EMF on antioxidant status and micronuclei in K562 cells and normal lymphocytes

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