Age‐related differential expression of apoptosis‐related genes in conjunctival epithelial cells

Giebel, J.; Woenckhaus, Christian; Fabian, Michael; Tost, Frank

doi:10.1111/j.1600-0420.2005.00472.x

Cited by 26 publications

(14 citation statements)

References 27 publications

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“…This finding cannot explain its abundant presence at 2-cell stage. However, APAF1 is also responsible for apoptosis in oocytes and surrounding granulosa cells in growing follicles [92]–[94]. As the IVF system is based on in vitro maturation of oocytes (aspirated from tertiary follicles), one can speculate that some of these oocytes have been already determined to undergo degradation by accumulation of APAF1 .…”

Section: Resultsmentioning

confidence: 99%

RNA Profiles of Porcine Embryos during Genome Activation Reveal Complex Metabolic Switch Sensitive to In Vitro Conditions

et al. 2013

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Fertilization is followed by complex changes in cytoplasmic composition and extensive chromatin reprogramming which results in the abundant activation of totipotent embryonic genome at embryonic genome activation (EGA). While chromatin reprogramming has been widely studied in several species, only a handful of reports characterize changing transcriptome profiles and resulting metabolic changes in cleavage stage embryos. The aims of the current study were to investigate RNA profiles of in vivo developed (ivv) and in vitro produced (ivt) porcine embryos before (2-cell stage) and after (late 4-cell stage) EGA and determine major metabolic changes that regulate totipotency. The period before EGA was dominated by transcripts responsible for cell cycle regulation, mitosis, RNA translation and processing (including ribosomal machinery), protein catabolism, and chromatin remodelling. Following EGA an increase in the abundance of transcripts involved in transcription, translation, DNA metabolism, histone and chromatin modification, as well as protein catabolism was detected. The further analysis of members of overlapping GO terms revealed that despite that comparable cellular processes are taking place before and after EGA (RNA splicing, protein catabolism), different metabolic pathways are involved. This strongly suggests that a complex metabolic switch accompanies EGA. In vitro conditions significantly altered RNA profiles before EGA, and the character of these changes indicates that they originate from oocyte and are imposed either before oocyte aspiration or during in vitro maturation. IVT embryos have altered content of apoptotic factors, cell cycle regulation factors and spindle components, and transcription factors, which all may contribute to reduced developmental competence of embryos produced in vitro. Overall, our data are in good accordance with previously published, genome-wide profiling data in other species. Moreover, comparison with mouse and human embryos showed striking overlap in functional annotation of transcripts during the EGA, suggesting conserved basic mechanisms regulating establishment of totipotency in mammalian development.

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Section: Resultsmentioning

confidence: 99%

RNA Profiles of Porcine Embryos during Genome Activation Reveal Complex Metabolic Switch Sensitive to In Vitro Conditions

et al. 2013

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“…A spectrum of ocular surface disease can then result—ranging from mild dry eye, to particularly painful symptoms associated with filament and mucous plaque formation. With aging, the number of goblet cells remains unchanged; however, the cell functions decline [25]; in addition, the aging conjunctival cells are more prone to apoptosis [26]. In the setting of dry eyes in older adults, a cumulative higher loss of functional goblet cells and increased level of goblet cell apoptosis occur which can lead to advanced DED.…”

Section: Aqueous Tear Deficiency Changes With Agingmentioning

confidence: 99%

Aging: A Predisposition to Dry Eyes

Sharma

Hindman

2014

Journal of Ophthalmology

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Dry eye syndrome is a disease of the ocular surface and tear film that is prevalent in older adults. Even though the degree of visual acuity loss in dry eye patients is commonly mild-to-moderate, in the aging population, this minimal change in visual status can lead to a significant decrease in visual function and quality of life. A healthy ocular surface is maintained by appropriate tear production and tear drainage, and deficiencies in this delicate balance can lead to dryness. In the aging eye, risk factors such as polypharmacy, androgen deficiency, decreased blink rates, and oxidative stress can predispose the patient to developing dry eye that is frequently more severe, has higher economic costs, and leads to worse consequences to the well-being of the patient. Understanding why elderly patients are at higher risk for developing dry eyes can provide insights into the diagnosis and management of the growing number of older adults struggling with dry eye and minimize the burden of disease on our aging population.

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“…Mucin-producing goblet cells decrease their function with aging [19], and conjunctival cells become more susceptible to apoptosis [20]. This condition may lead to a decreased wettability of the ocular surface, which rapidly becomes dry, and to a diffuse damage of epithelial cells, finally leading to symptomatic eye dryness.…”

Section: Endogenous and Iatrogenic Causes Of Age-related Dry Eyementioning

confidence: 99%

Age-Related Dry Eye Lactoferrin and Lactobionic Acid

Rusciano¹,

Pezzino²,

Olivieri³

et al. 2018

Ophthalmic Res

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Dry eye is the most prominent pathology among those involving the ocular surface: a decrease of the aqueous (less frequent) or the lipid (more frequent) component of the tear film is the cause of the diminished stability of tears that is observed in this pathology. Dry eye shows a clear distribution linked to both sex (being more frequent among women) and age (increasing with aging). Therefore, specific treatments taking into account the etiology of the disease would be desired. The role of lactoferrin and its functional mimetic lactobionic acid are reported here as a possible remedy for age-related dry eye.

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Age‐related differential expression of apoptosis‐related genes in conjunctival epithelial cells

Cited by 26 publications

References 27 publications

RNA Profiles of Porcine Embryos during Genome Activation Reveal Complex Metabolic Switch Sensitive to In Vitro Conditions

RNA Profiles of Porcine Embryos during Genome Activation Reveal Complex Metabolic Switch Sensitive to In Vitro Conditions

Aging: A Predisposition to Dry Eyes

Age-Related Dry Eye Lactoferrin and Lactobionic Acid

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